Thermo Fisher Scientific Applied Biosystems TaqMan Gene Expression Master Mix

Thermo Fisher Scientific Applied Biosystems TaqMan Gene Expression Master Mix

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Product Details:

Storage Instructions Store at -20C
Accuracy High reproducibility in qPCR
Usage Type Laboratory Reagent
Instruments Type Real-Time PCR Systems
Shelf Life 12 months
Measurement Range Wide dynamic range
Function Quantitative gene expression detection
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Thermo Fisher Scientific Applied Biosystems TaqMan Gene Expression Master Mix Price And Quantity

Reaction Components Includes dNTPs, ROX Dye, Buffer, DNA polymerase
Package Size Typically 5mL or 50mL (varies by catalog number)
Application Compatible with TaqMan gene expression assays
Catalog Number Varies (e.g., 4369016, 4369017)
Sample Compatibility cDNA, total RNA, TaqMan assays
Temperature Stability Requires storage and transportation on ice/dry ice

Thermo Fisher Scientific Applied Biosystems TaqMan Gene Expression Master Mix Product Specifications

Use Gene expression analysis by real-time PCR
Operating Type Automated
Portable Yes
Material Proprietary chemical formulation
Technology qPCR (quantitative Polymerase Chain Reaction)
Shelf Life 12 months
Usage Type Laboratory Reagent
Instruments Type Real-Time PCR Systems
Accuracy High reproducibility in qPCR
Storage Instructions Store at -20C
Real-Time Operation Yes
Function Quantitative gene expression detection
Measurement Range Wide dynamic range
Equipment Type Gene Expression Master Mix
Condition New
Wall Mounted No
Color Clear/colorless
Features High specificity and sensitivity, Hot-start DNA polymerase, Optimized for TaqMan assays

Reaction Components Includes dNTPs, ROX Dye, Buffer, DNA polymerase
Package Size Typically 5mL or 50mL (varies by catalog number)
Application Compatible with TaqMan gene expression assays
Catalog Number Varies (e.g., 4369016, 4369017)
Sample Compatibility cDNA, total RNA, TaqMan assays
Temperature Stability Requires storage and transportation on ice/dry ice

Product Description

Brand	Thermo Fisher Scientific
Product Type	Master Mix
Pack Size	5 x 5 mL
CAT No	4369510
Application	Molecular Biology

Get precise and reliable real-time qPCR quantification over nine logs of linear dynamic range with TaqMan Gene Expression Master Mix. TaqMan Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target. Extended benchtop stability provides consistent results for automated liquid-handling systems, making it the choice for high-throughput sample processing, overnight or over the weekend. TaqMan Gene Expression Master Mix has been validated with TaqMan gene expression assays and Applied Biosystems real-time systems, providing precise quantification for a variety of gene expressionabased real-time qPCR applications.

Optimized for Reliable qPCR Performance

This gene expression master mix is meticulously formulated for real-time quantitative PCR applications. Featuring a robust hot-start DNA polymerase and ROX dye for normalization, Thermo Fisher's TaqMan Master Mix ensures high specificity and superior sensitivity, resulting in accurate data across a wide dynamic range. It is designed for seamless integration with TaqMan assays, supporting various real-time PCR instruments.

Versatile Compatibility for Gene Expression Analysis

Engineered for flexibility, the TaqMan Gene Expression Master Mix is compatible with cDNA and total RNA samples, making it suitable for a broad range of experimental needs. Whether you are conducting gene expression studies or validating results, its wide measurement range and high reproducibility make it indispensable for researchers and clinical laboratories alike.

Safe Handling and Storage

To maintain reagent stability and integrity, it is recommended to store the master mix at -20C and transport it on ice or dry ice. This ensures reliable performance for up to 12 months from manufacture. The clear, colorless formulation is easy to identify and dispense, facilitating efficient laboratory workflows even in high-throughput environments.

FAQ's of Thermo Fisher Scientific Applied Biosystems TaqMan Gene Expression Master Mix:

Q: How should the TaqMan Gene Expression Master Mix be stored and handled?

A: The master mix should be stored at -20C to preserve its integrity and ensure optimal performance. During transportation, it must be kept on ice or dry ice. Avoid repeated freeze-thaw cycles and always return unused portions to the freezer promptly.

Q: What applications is this master mix compatible with?

A: It is designed for use with TaqMan gene expression assays and is suitable for qPCR analysis of cDNA and total RNA samples. The mix supports a wide array of gene expression research and diagnostic workflows.

Q: When should I choose this master mix for my experiments?

A: Select the TaqMan Gene Expression Master Mix when high sensitivity, precise quantification, and reliable reproducibility are crucial, especially in real-time PCR-based gene expression measurements.

Q: Where can this master mix be used?

A: This reagent is suitable for use in molecular biology laboratories, research institutions, clinical diagnostic labs, and facilities equipped with real-time PCR systems that support TaqMan assays.

Q: What benefits does this master mix offer over standard PCR mixes?

A: It provides high specificity, sensitivity, and reproducibility due to its proprietary formulation with hot-start DNA polymerase and ROX dye. These features reduce background signal and increase detection accuracy across a broad dynamic range.

Q: What is the process for using this master mix in qPCR?

A: Simply add the master mix to your prepared cDNA or RNA samples, along with TaqMan assay reagents, and load the reaction into a compatible real-time PCR instrument. The mix is ready-to-use, streamlining the setup of automated or manual qPCR workflows.

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