Product Description
| Brand |
Thermo Fisher Scientific |
| Product Type |
Master Mix |
| Pack Size |
2 x 50 mL |
| CAT No |
A30872 |
| Application |
Molecular Biology |
TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples. It is designed to deliver sensitive and confident results from genomic DNA targets on a broad range of qPCR instrument platforms. TaqPath ProAmp Master Mix is manufactured under a strong ISO13485 quality management system and is designed to produce consistent product performance lot after lot.
Precision Amplification for qPCR and PCRTaqPath ProAmp Master Mix offers superior DNA amplification fidelity and robust performance for demanding laboratory applications. Compatible with most standard and fast qPCR cyclers, it reliably delivers high specificity and reduced inhibition, enabling confident gene expression analysis and genotyping. Its proprietary enzyme blend and optimized buffer formulation provide consistent results even when dealing with complex samples or rapid cycling protocols.
Ready-to-Use Convenience for LaboratoriesThis master mix simplifies PCR reaction setup-just add your primers, probes, and DNA template. Packaged in clear, aliquot-friendly screw-cap tubes and stable at -20C, Applied Biosystems TaqPath ProAmp Master Mix is designed for efficiency and ease in laboratory workflows. With multiple pack sizes and a long shelf life, labs can minimize waste and streamline routine PCR and real-time PCR experiments.
FAQ's of Thermo Fisher Scientific Applied Biosystems TaqPath ProAmp Master Mix:
Q: How should the TaqPath ProAmp Master Mix be stored to maintain its activity?
A: Store the TaqPath ProAmp Master Mix at -20C immediately upon receipt. This ensures enzyme stability and preserves the optimized buffer properties, maintaining its robust performance for up to 12 months.
Q: What steps are involved in setting up a PCR reaction using this master mix?
A: To set up a PCR reaction, simply mix the 2X concentrated master mix with your primers or probes and DNA template. No additional buffer optimization is necessary. The ready-to-use formulation minimizes pipetting steps and variability between samples.
Q: When can I use the TaqPath ProAmp Master Mix in my workflow?
A: This master mix is suitable for real-time PCR (qPCR), standard PCR, gene expression analysis, and genotyping at any stage where precise DNA amplification is needed. It performs well in standard and fast cycling protocols with high compatibility across thermal cyclers.
Q: Where can I use the TaqPath ProAmp Master Mix?
A: It is ideal for laboratory settings involved in molecular biology research, diagnostics, or genetic testing. Its portability and easy reagent tube format make it convenient for use in research labs, clinical labs, and academic institutions.
Q: What are the main benefits of using this master mix over other alternatives?
A: Key benefits include high specificity, enhanced amplification fidelity, reduced PCR inhibition, and broad compatibility with both standard and fast-cycling qPCR instruments. Its convenience and reliability streamline laboratory workflows and ensure accurate experimental results.
Q: How does the optimized buffer and enzyme blend improve PCR results?
A: The proprietary enzyme blend and buffer system are engineered for Taq DNA polymerase activity, promoting efficient hot-start amplification, high yield, and minimal nonspecific product formation, enabling clear, reproducible results across various DNA samples.