Qiagen RNeasy Protect Mini Kit (250)

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Product Details:

Function RNA stabilization and purification
Usage Type Laboratory/Reagent
Instruments Type Molecular Biology Kit
Storage Instructions Store at room temperature (1525C) or as specified by the manufacturer
Features Immediate RNA stabilization, high-quality RNA yield, fast and convenient protocol
Shelf Life As per manufacturer, typically 1-2 years
Equipment Type RNA Isolation Kit
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Qiagen RNeasy Protect Mini Kit (250) Price And Quantity

Qiagen RNeasy Protect Mini Kit (250) Product Specifications

Technology Silica-membrane technology
Portable Yes
Wall Mounted No
Instruments Type Molecular Biology Kit
Storage Instructions Store at room temperature (1525C) or as specified by the manufacturer
Function RNA stabilization and purification
Real-Time Operation No
Material High-quality reagents and buffers
Usage Type Laboratory/Reagent
Color White/Colorless reagents
Equipment Type RNA Isolation Kit
Condition New
Operating Type Manual
Use Purification and stabilization of total RNA
Shelf Life As per manufacturer, typically 1-2 years
Features Immediate RNA stabilization, high-quality RNA yield, fast and convenient protocol

Product Description

Brand Qiagen

Product Type RNA Kit

Pack Size 250T

CAT No 74126

Application Molecular Reagent

RNeasy Protect Mini Kits enable stabilization of RNA in tissue samples, RNA and DNA in sorted or cultured cells, RNA in human saliva samples and RNA in bacterial samples. The RNeasy Protect Mini Kit and Bacteria Mini Kit include RNeasy spin columns for purifying up to 100 g of high-quality RNA using silica-membrane technology. The RNeasy Protect Cell Mini Kit provides the RNeasy Plus Mini Kit for purification of total RNA. The RNeasy Protect Saliva Mini Kit includes the RNeasy Micro Kit, which purifies and concentrates total RNA using specialized RNeasy MinElute spin columns.

Immediate RNA Stabilization

This kit features an integrated stabilization step that preserves RNA integrity from the moment of sample collection. Protecting RNA from degradation ensures that researchers obtain reliable and reproducible results, making this kit particularly suitable for sensitive downstream applications such as gene expression analysis.

Efficient Purification Protocol

The RNeasy Protect Mini Kit streamlines the RNA purification process to less than 30 minutes. The manual protocol is straightforward, requiring no specialized equipment, and is optimized for a variety of sample types. With an elution volume range of 30-100 l, researchers can tailor RNA concentration to their experimental needs.

High-Quality Yields for Diverse Samples

Capable of extracting RNA from animal tissues, cultured cells, bacteria, yeast, and plants, this versatile kit addresses the needs of molecular biology laboratories handling a broad range of biological material. Each prep can yield up to 100 g of RNA, suitable for demanding downstream applications in genomics and transcriptomics.

FAQ's of Qiagen RNeasy Protect Mini Kit (250):

Q: How does the Qiagen RNeasy Protect Mini Kit (250) stabilize RNA during extraction?

A: The kit employs a specialized stabilization reagent that preserves RNA integrity by immediately inactivating RNases in the sample at the time of collection. This ensures high-quality RNA and prevents degradation before purification.

Q: What types of samples can be processed with this kit?

A: It is designed for use with a variety of biological materials, including animal tissues, cultured cells, bacteria, yeast, and plant material. This versatility allows comprehensive RNA extraction from a wide range of sources.

Q: When should researchers use the RNeasy Protect Mini Kit?

A: This kit is ideal when immediate RNA stabilization and high yield are crucial, such as prior to gene expression analysis, RT-PCR, qPCR, microarray, or RNA-seq. Its fast protocol is suited for laboratories looking to efficiently process multiple samples.

Q: Where should the kit be stored to ensure optimal performance?

A: Store the kit at room temperature (15-25C) or as specified by the manufacturer. Proper storage extends shelf life and maintains reagent efficacy.

Q: What is the typical RNA yield and elution volume per preparation?

A: Each preparation can yield up to 100 g of total RNA, depending on sample type. The final RNA is eluted in 30-100 l, allowing flexibility for different downstream applications.

Q: Which downstream applications are compatible with RNA isolated using this kit?

A: RNA purified using the Qiagen RNeasy Protect Mini Kit is compatible with several molecular techniques, including RT-PCR, quantitative PCR, microarray analysis, and RNA sequencing (RNA-seq).

Q: What are the key benefits of using this RNA isolation kit in molecular biology workflows?

A: The key advantages include immediate RNA stabilization, rapid processing in under 30 minutes, high RNA yields, and broad compatibility with various biological samples and analytical techniques. This ensures reliable results and efficient laboratory workflows.

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