Qiagen miRCURY LNA SYBR Green PCR Kit

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Product Details:

Shelf Life 12 months from date of manufacture (when stored correctly)
Measurement Range Wide dynamic range for miRNA quantification
Usage Type Laboratory Research Use Only
Accuracy High specificity and sensitivity due to LNA technology
Function Detects and quantifies microRNAs in real-time PCR assays
Instruments Type Compatible with standard real-time PCR cyclers (e.g., Roche LightCycler, Applied Biosystems, Bio-Rad, etc.)
Features Sensitive, accurate, and validated for miRNA qPCR with LNA technology
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Qiagen miRCURY LNA SYBR Green PCR Kit Price And Quantity

Qiagen miRCURY LNA SYBR Green PCR Kit Product Specifications

Storage Instructions Store at -20C
Wall Mounted No
Features Sensitive, accurate, and validated for miRNA qPCR with LNA technology
Condition New
Color Clear or Colorless solutions
Power Source Not Applicable (consumable kit)
Instruments Type Compatible with standard real-time PCR cyclers (e.g., Roche LightCycler, Applied Biosystems, Bio-Rad, etc.)
Operating Type Manual/Automated PCR instrument compatible
Equipment Type PCR Kit
Material Reagents and Enzymes
Function Detects and quantifies microRNAs in real-time PCR assays
Accuracy High specificity and sensitivity due to LNA technology
Usage Type Laboratory Research Use Only
Technology qPCR (quantitative PCR) with LNA-enhanced SYBR Green detection
Measurement Range Wide dynamic range for miRNA quantification
Portable Yes (kit format)
Real-Time Operation Yes
Use miRNA Quantification and Expression Analysis
Shelf Life 12 months from date of manufacture (when stored correctly)

Product Description

Brand Qiagen

Product Type PCR Kits

Pack Size 200T

CAT No 339345

Application Molecular Reagent

The miRCURY LNA SYBR Green PCR Kit contains a high-performance PCR master mix specifically designed for use with themiRCURY LNA miRNA PCR System. The miRCURY SYBR Green PCR Kit also features a built-in control for visual identification of correct template addition, as well as Q-Bond, an additive in the PCR buffer that enables short cycling steps without loss of PCR sensitivity and efficiency. The kit has been optimized for use with any real-time cycler. ROX Reference Dye is provided in a separate tube and can be added if your cycler requires ROX as a passive reference dye.

Reliable and Accurate miRNA Quantification

The miRCURY LNA SYBR Green PCR Kit enables researchers to achieve accurate and sensitive detection of microRNAs using advanced LNA technology. Each reaction leverages highly specific HotStart DNA Polymerase, ensuring unparalleled detection of even low-abundance miRNAs. The kit offers stable shelf life and provides reliable, reproducible results for expression analysis applications.

Comprehensive Kit Components for Seamless Workflow

Supplied with a 2x SYBR Green Master Mix, ROX reference dye, PCR-grade water, and an easy-to-follow protocol leaflet, the kit simplifies experimental setup. Its format supports both manual and automated qPCR platforms, catering to diverse laboratory needs. The reagents are colorless, reflect high purity, and are compatible with popular real-time PCR systems, ensuring flexibility and ease of use.

Optimized for Laboratory Research Use Only

Intended solely for research use, the miRCURY LNA SYBR Green PCR Kit excels at miRNA quantification and validation projects. It must be stored at -20C for best performance and retains stability after multiple freeze-thaw cycles. Researchers in academic, clinical, and industrial labs benefit from its robust design and validated, high-specificity performance, making it ideal for miRNA-focused expression studies.

FAQ's of Qiagen miRCURY LNA SYBR Green PCR Kit:

Q: How do I use the Qiagen miRCURY LNA SYBR Green PCR Kit for miRNA quantification?

A: To quantify miRNAs, synthesize cDNA from purified RNA samples, then set up your qPCR reactions using the included master mix, ROX dye, PCR-grade water, and your specific primers. Run the reactions on a compatible real-time PCR instrument following the provided protocol.

Q: What are the main benefits of using this kit for microRNA research?

A: This kit offers high sensitivity and specificity due to LNA-enhanced technology, a wide dynamic range for detecting both low and high-abundance miRNAs, and reliable performance after multiple freeze-thaw cycles, making it ideal for accurate miRNA profiling and validation.

Q: When should the kit components be stored and how?

A: All kit components should be stored at -20C immediately upon arrival. The kit is stable for up to 12 months from the date of manufacture if stored correctly, and remains effective after several freeze-thaw events.

Q: Where can this PCR kit be used within the workflow?

A: The Qiagen miRCURY LNA SYBR Green PCR Kit is suitable for laboratory research settings requiring miRNA quantification. It is compatible with standard real-time PCR cyclers, making it versatile for academic, clinical, and industrial research facilities.

Q: What sample types are compatible with this kit for miRNA analysis?

A: The kit is specifically designed for use with cDNA that has been synthesized from purified RNA samples, ensuring precise and consistent quantification of miRNAs.

Q: How does the kit ensure high accuracy and specificity during detection?

A: Incorporating LNA (locked nucleic acid) technology and HotStart DNA Polymerase, the kit significantly enhances specificity and sensitivity for miRNA detection, minimizing background and producing reproducible results even with challenging targets.

Q: What is the recommended reaction volume and instrument compatibility?

A: Each reaction is recommended at a final volume of 10-20 l. The kit works with most real-time PCR instruments, including popular models from Roche, Applied Biosystems, and Bio-Rad.

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