Qiagen QIAamp Circulating Nucleic Acid Kit (50)

Qiagen QIAamp Circulating Nucleic Acid Kit (50)

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Product Details:

Features High yield, easy protocol, fast processing, suitable for downstream molecular analyses
Function Extraction and purification of circulating nucleic acids
Usage Type Laboratory/Research Use Only
Instruments Type Manual extraction kit
Shelf Life 12-24 months from date of manufacture
Accuracy Consistent high-quality nucleic acid yield
Storage Instructions Store at 28C in a dry place
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Qiagen QIAamp Circulating Nucleic Acid Kit (50) Price And Quantity

Applications Liquid biopsy, cancer monitoring, prenatal screening, virus detection
Kit Content QIAamp Mini columns, collection tubes, buffers, proteinase K, carrier RNA, instructions for use
Process Time Approximately 60 minutes
Compatibility Compatible with downstream qPCR, digital PCR, NGS and other molecular assays
Sample Types Human plasma, serum, urine, cell-free body fluids
Elution Volume 20150 l
Throughput Up to 50 preps per kit
Sample Volume Input 200 l to 5 ml plasma or serum per prep

Qiagen QIAamp Circulating Nucleic Acid Kit (50) Product Specifications

Measurement Range Sensitive detection of nucleic acids down to picogram levels
Portable Yes
Use For isolation and purification of circulating cell-free nucleic acids (cfDNA, cfRNA) from plasma or serum
Noise Level Silent Operation
Equipment Type Nucleic Acid Extraction Kit
Storage Instructions Store at 28C in a dry place
Accuracy Consistent high-quality nucleic acid yield
Condition New
Material Plastic (columns), reagents (various chemicals)
Dimension (L*W*H) Standard kit box dimensions (varies by packaging batch)
Operating Type Manual
Real-Time Operation No
Wall Mounted No
Color Kit with color-coded reagents and columns
Shelf Life 12-24 months from date of manufacture
Technology Silica membrane technology
Weight Approx. 1.1 kg (for 50 preps)
Instruments Type Manual extraction kit
Features High yield, easy protocol, fast processing, suitable for downstream molecular analyses
Usage Type Laboratory/Research Use Only
Function Extraction and purification of circulating nucleic acids

Applications Liquid biopsy, cancer monitoring, prenatal screening, virus detection
Kit Content QIAamp Mini columns, collection tubes, buffers, proteinase K, carrier RNA, instructions for use
Process Time Approximately 60 minutes
Compatibility Compatible with downstream qPCR, digital PCR, NGS and other molecular assays
Sample Types Human plasma, serum, urine, cell-free body fluids
Elution Volume 20150 l
Throughput Up to 50 preps per kit
Sample Volume Input 200 l to 5 ml plasma or serum per prep

Product Description

Brand Qiagen

Product Type DNA/ RNA Isolation Kits

Pack Size 50 Nos

CAT No 55114

Application Molecular Reagent

The QIAamp Circulating Nucleic Acid Kit greatly simplifies concentration and purification of free-circulating DNA and RNA from plasma or serum. The kit can be automated on the QIAcube Connect.

Streamlined Extraction of Cell-Free Nucleic Acids

Harnessing advanced silica membrane technology, the QIAamp Circulating Nucleic Acid Kit provides high-yield and high-purity nucleic acid isolation from small to large plasma or serum volumes. Its easy-to-follow protocol minimizes hands-on time and reduces user error, making it ideal for routine laboratory workflows.

Reliable Performance for Sensitive Molecular Applications

With the ability to detect nucleic acids down to picogram levels, this kit is optimized for sensitive applications such as liquid biopsy and retrospective disease monitoring. Users benefit from a consistent yield and purity across various preparation volumes, suitable for both research and clinical investigation settings.

Flexible Applications and Easy Integration

The kit's compatibility with downstream qPCR, digital PCR, and NGS allows for seamless integration into numerous research protocols. Its color-coded reagents and convenient format empower laboratories to efficiently process up to 50 samples per kit with confidence and reproducibility.

FAQ's of Qiagen QIAamp Circulating Nucleic Acid Kit (50):

Q: How does the QIAamp Circulating Nucleic Acid Kit extract nucleic acids from plasma or serum?

A: The kit uses silica membrane technology within spin columns to bind and purify cell-free nucleic acids from plasma, serum, or other body fluids after lysis and proteinase K digestion. This manual process ensures high recovery and purity in approximately 60 minutes.

Q: What sample types and volumes can be processed with the kit?

A: The kit is validated for human plasma, serum, urine, and other cell-free body fluids. You can process input volumes ranging from 200 l to 5 ml per preparation, accommodating a wide range of research and diagnostic needs.

Q: When should I use this kit in my workflow for molecular testing?

A: Use the QIAamp Circulating Nucleic Acid Kit as the initial step to purify and concentrate cfDNA or cfRNA from body fluids before performing downstream molecular analyses such as qPCR, digital PCR, or next-generation sequencing (NGS).

Q: Where should the kit be stored and how long does it last?

A: Store the kit in a dry place at 2-8C to maintain reagent stability. It features a shelf life of 12 to 24 months from the date of manufacture, ensuring long-term usability for laboratory inventory management.

Q: What are the benefits of using this kit for liquid biopsy or clinical research?

A: The kit is designed for sensitive and efficient recovery of circulating nucleic acids, supporting applications in cancer monitoring, prenatal screening, and virus detection. Its high yield, reproducibility, and compatibility with common molecular assays yield reliable results.

Q: Is special equipment required for using this kit?

A: No, the kit is a manual extraction system and does not require automated equipment. Standard lab equipment such as a microcentrifuge and pipettes are sufficient for the process.

Q: Can I use extracted nucleic acids from this kit directly for qPCR or NGS?

A: Yes, nucleic acids purified with this kit are compatible with qPCR, digital PCR, NGS, and other molecular assays, providing high-quality material suitable for sensitive downstream analysis.

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