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Qiagen RNeasy UCP Micro Kit Qiagen RNeasy UCP Micro Kit Qiagen RNeasy UCP Micro Kit
Qiagen RNeasy UCP Micro Kit
Qiagen RNeasy UCP Micro Kit Qiagen RNeasy UCP Micro Kit

Qiagen RNeasy UCP Micro Kit

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Product Details:

  • Accuracy Yields highly pure RNA suitable for sensitive applications
  • Shelf Life 12 months
  • Measurement Range Sample sizes: 15 10 cells or <5 mg tissue
  • Usage Type Laboratory/Research
  • Storage Instructions Store at 28C for long-term; some components at room temperature
  • Features Ultraclean production, DNase-free, highly pure RNA, fast processing
  • Function Isolation and purification of RNA
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Qiagen RNeasy UCP Micro Kit Price And Quantity

Qiagen RNeasy UCP Micro Kit Product Specifications

  • Ultraclean production, DNase-free, highly pure RNA, fast processing
  • Isolation and purification of RNA
  • RNA Extraction Kit
  • Yes
  • Life Science Lab Consumables
  • New
  • Spin column-based technology
  • No
  • Yields highly pure RNA suitable for sensitive applications
  • 12 months
  • No
  • Sample sizes: 15 10 cells or <5 mg tissue
  • Purification of total RNA from small samples
  • Plastic, Reagents, Silica membrane
  • Laboratory/Research
  • Manual
  • Store at 28C for long-term; some components at room temperature
  • White and transparent (kit components)

Product Description

Brand Qiagen

Product Type RNA Kit

Pack Size 50T

CAT No 73934



The RNeasy UCP Micro Kit is designed for purification of up to 45 g RNA from small or low biomass samples. The spin columns and buffers are treated to remove exogenous nucleic acids. RNeasy UCP Micro technology combines the selective binding properties of an ultra-clean, silica-based membrane with the speed of microspin technology. Ultra-clean guanidine-thiocyanatecontaining lysis buffer RULT and ethanol are added to the sample to create conditions that promote selective binding of RNA to the RNeasy UCP MinElute membrane. The sample is then applied to the RNeasy UCP MinElute spin column and RNA binds to the silica membrane. Traces of DNA that may copurify are removed by DNase treatment on the RNeasy UCP MinElute spin column. DNase and any contaminants are washed away with ultra-clean wash buffers RUWT and RUPE, and high-quality total RNA is eluted in ultra-clean water (see figuresLinearity over a broad dilution rangeandRNeasy UCP Micro Kit Procedure). With the RNeasy UCP Micro procedure, all RNA molecules longer than 200 nucleotides are purified. The procedure enriches for mRNA, since most RNAs <200 nucleotides are selectively excluded. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system.




Exceptional RNA Purity and Reliability

The RNeasy UCP Micro Kit ensures the ultraclean production of high-quality RNA. It includes DNase-free reagents and advanced silica membrane spin column technology, resulting in consistent yields ideal for sensitive downstream applications such as real-time PCR or gene expression analysis. Researchers benefit from increased confidence in their molecular biology studies thanks to the removal of contaminants and high reproducibility.


Convenient and Fast Workflow

Designed for efficiency, the kit enables the isolation and purification of RNA in just around 30 minutes. All necessary plastic materials and reagents are provided, and the process is straightforward, accommodating small sample inputs ranging from 1-5 x 105 cells or less than 5 mg tissue. Minimal elution volume (as low as 10 l) maximizes concentration, supporting applications where sample availability is limited.

FAQ's of Qiagen RNeasy UCP Micro Kit:


Q: How does the QIAGEN RNeasy UCP Micro Kit isolate RNA from small samples?

A: The kit uses a spin column-based technology with an ultraclean silica membrane that binds RNA during purification. After lysing your sample (cells or tissue), RNA selectively adheres to the membrane, while impurities are washed away using provided buffers. Elution with RNase-free water releases highly pure RNA ready for downstream use.

Q: What types of samples can I process with this kit?

A: This kit is suitable for total RNA purification from up to 5 x 105 cells, less than 5 mg of tissue, or microdissected material, making it ideal for experiments with limited or precious biological samples.

Q: When is the ideal time to use the RNeasy UCP Micro Kit in my workflow?

A: You should use the kit during the initial stages of your molecular biology workflow when you need to obtain high-quality RNA as a starting material for applications such as RT-PCR, gene expression studies, or microarray analysis.

Q: Where can the QIAGEN RNeasy UCP Micro Kit be stored and what are the storage requirements?

A: The majority of components should be stored at 2-8C for long-term stability, though some can be safely stored at room temperature. Always refer to the kit instructions for specific storage guidelines to maintain optimal reagent performance.

Q: What benefits does this kit offer for gene expression and microarray applications?

A: The RNeasy UCP Micro Kit provides RNA of exceptional purity and free of DNase contamination, ensuring accurate results even in sensitive applications like gene expression analysis or microarrays, where contaminants can compromise data quality.

Q: How long does the RNA extraction process take and is the kit portable?

A: The complete extraction protocol can be finished in about 30 minutes. Since the kit includes all necessary consumables and is compact in design, it can be conveniently transported for use in various laboratory locations.

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