Qiagen Hot StarTaq Master Mix Kit (2500 U)

Qiagen Hot StarTaq Master Mix Kit (2500 U)

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Product Details:

Function DNA amplification by PCR
Instruments Type PCR Reagent Kit
Features High specificity, convenient master mix, reduced setup time, HotStart Taq DNA Polymerase
Storage Instructions Store at -20C, avoid repeated freeze-thaw cycles
Measurement Range Suitable for standard PCR reaction volumes (1050 l)
Shelf Life 12 months (When stored as recommended)
Accuracy High fidelity for research PCR (not for diagnostic use)
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Qiagen Hot StarTaq Master Mix Kit (2500 U) Price And Quantity

Qiagen Hot StarTaq Master Mix Kit (2500 U) Product Specifications

Instruments Type PCR Reagent Kit
Function DNA amplification by PCR
Features High specificity, convenient master mix, reduced setup time, HotStart Taq DNA Polymerase
Wall Mounted No
Noise Level Silent Operation
Dimension (L*W*H) Kit format (no fixed physical dimension)
Material Biological Reagents (Enzymes, dNTPs, Buffer)
Portable Yes (as a kit)
Real-Time Operation No (for end-point PCR applications)
Color Clear to light yellow (solution form)
Equipment Type PCR Master Mix Kit
Shelf Life 12 months (When stored as recommended)
Use Amplification of DNA templates by PCR
Operating Type Manual (for use in thermocyclers)
Measurement Range Suitable for standard PCR reaction volumes (1050 l)
Storage Instructions Store at -20C, avoid repeated freeze-thaw cycles
Accuracy High fidelity for research PCR (not for diagnostic use)
Technology HotStart PCR Technology
Usage Type Laboratory Research
Weight Approximately kit-dependent, typically <1 kg
Power Source Not Required (uses laboratory thermocycler)
Condition New

Product Description

Brandc Qiagen

Product Type Master Mix

Pack Size 2500 ul

CAT No 203446

Application Molecular Reagent

HotStarTaq Master Mix contains HotStarTaq DNA Polymerase, the unique QIAGEN PCR Buffer that minimizes the requirement for optimization, and dNTPs. Providing all components in a master mix reduces pipetting steps and the risk of contamination, while increasing throughput and reproducibility.

Reliable High-Specificity PCR Amplification

HotStarTaq Master Mix uses advanced HotStart PCR technology to deliver highly specific DNA amplification, even with complex or GC-rich templates. The pre-optimized mix ensures robust and reproducible results for a range of PCR applications in research laboratories.

Streamlined Setup and Reduced Contamination Risk

Pre-assembled with DNA polymerase, dNTPs, and buffer, the master mix minimizes pipetting steps and reduces the potential for handling errors or contamination. Simply add your primers and DNA templates for efficient setup without the need for extensive reagent preparation.

Long Shelf Life and Easy Storage

The kit offers a shelf life of up to 12 months when properly stored at -20C. Its biological reagent formulation is stable and portable, making it a reliable choice for laboratories needing consistent and high-yield PCR results over time.

FAQ's of Qiagen Hot StarTaq Master Mix Kit (2500 U):

Q: How do I use the Qiagen HotStarTaq Master Mix Kit for PCR amplification?

A: To use the kit, simply add your template DNA and primers to the HotStarTaq Master Mix. The mix already contains the enzyme, dNTPs, and buffer. After adding your sample, initiate activation by heating at 95C for 15 minutes, then proceed with standard PCR cycling protocols.

Q: What types of PCR applications is this kit suitable for?

A: This kit excels in routine PCR, complex amplification tasks, genotyping, and working with GC-rich templates. Its high specificity and robust buffer system make it ideal for a wide range of molecular research endeavors.

Q: When and how should I store the HotStarTaq Master Mix Kit to maintain performance?

A: Store the kit at -20C immediately upon arrival. Avoid repeated freeze-thaw cycles to ensure consistent performance over its 12-month shelf life. Always keep reagents on ice during use for optimal results.

Q: What are the advantages of using a HotStart PCR technology master mix?

A: HotStart PCR technology minimizes non-specific amplification and primer-dimer formation by keeping the enzyme inactive at low temperatures. This translates to higher specificity, improved yields, and greater reproducibility in your PCR results.

Q: Where can this master mix kit be used, and with what equipment?

A: This kit is designed for laboratory research use in manual thermocyclers. Its versatile formulation is compatible with most standard PCR instruments found in research, educational, and clinical research labs.

Q: What are the key benefits of this master mix compared to preparing separate PCR reagents?

A: The master mix simplifies preparation by combining necessary reagents, saving time and reducing contamination risk. It ensures lot-to-lot consistency, decreases pipetting errors, and streamlines your PCR workflow for higher efficiency.

Q: Is the Qiagen HotStarTaq Master Mix Kit intended for diagnostic or real-time PCR applications?

A: No, this reagent kit is intended strictly for research use and end-point PCR applications. It is not suitable for diagnostic use or real-time PCR, as it lacks intercalating dyes or probe systems.

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