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QIAGEN Plasmid Plus Maxi Kit (100) QIAGEN Plasmid Plus Maxi Kit (100) QIAGEN Plasmid Plus Maxi Kit (100)
QIAGEN Plasmid Plus Maxi Kit (100)
QIAGEN Plasmid Plus Maxi Kit (100) QIAGEN Plasmid Plus Maxi Kit (100)

QIAGEN Plasmid Plus Maxi Kit (100)

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Product Details:

  • Shelf Life 12 months
  • Accuracy High quality DNA yield (A260/A280 ratio: 1.801.90)
  • Measurement Range For 150750 ml E. coli culture per prep
  • Usage Type Laboratory Use
  • Storage Instructions Store at room temperature (1525C)
  • Features Rapid purification, high yield, high purity, scalable
  • Function Plasmid DNA extraction and purification
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QIAGEN Plasmid Plus Maxi Kit (100) Price And Quantity

QIAGEN Plasmid Plus Maxi Kit (100) Product Specifications

  • No
  • High-quality plastic components, pre-filled buffer tubes
  • Yes
  • Life Sciences Research Kit
  • Isolation and purification of plasmid DNA from bacterial cultures
  • Rapid purification, high yield, high purity, scalable
  • White/Blue
  • No
  • Plasmid DNA extraction and purification
  • Anion-exchange silica membrane technology
  • Store at room temperature (1525C)
  • Laboratory Use
  • For 150750 ml E. coli culture per prep
  • New
  • Manual
  • 12 months
  • High quality DNA yield (A260/A280 ratio: 1.801.90)
  • Plasmid DNA Purification Kit

Product Description

Brand                       Qiagen

Product Type           PCR Kit

Pack Size                 100T

CAT No                     12965

Application              Molecular Reagent

The QIAGEN Plasmid Plus Kits enable ultrafast, large-scale purification of up to 10 mg of highly pure plasmid DNA. The use of a vacuum manifold allows purification of up to 24 samples in parallel, reducing hands-on time. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. The QIAGEN Plasmid Plus Kits also feature a dedicated wash buffer for endotoxin reduction. The plasmid DNA obtained is highly suitable for a multitude of applications, including transfection into sensitive cell lines.



Efficient Plasmid DNA Isolation

The QIAGEN Plasmid Plus Maxi Kit streamlines the plasmid purification process, enabling researchers to extract high-quality DNA efficiently from E. coli cultures. Using innovative anion-exchange silica membrane technology, the kit ensures optimal yield and purity in less than 45 minutes while eliminating the complexity often associated with traditional methods. Its high performance supports a wide range of molecular biology applications.


Flexible Applications and Scalability

Designed for flexibility, this kit is suitable for transfection, cloning, sequencing, restriction enzyme digestion, and PCR. With the capacity to process up to 100 maxi preps from culture volumes ranging from 150 to 750 ml, it is ideal for laboratories with evolving project demands. Consistent, high DNA yield and purity make it an essential tool for biotechnology and life sciences research.


User-Friendly and Reliable Kit Components

The kit comes complete with pre-filled buffer tubes, high-quality plastic columns, collection tubes, and an easy-to-follow user manual. All components are color-coded and labeled for straightforward handling. Its shelf life of 12 months and room temperature storage facilitate convenient laboratory management and continuous workflow optimization.

FAQ's of QIAGEN Plasmid Plus Maxi Kit (100):


Q: How does the QIAGEN Plasmid Plus Maxi Kit purify plasmid DNA from E. coli cultures?

A: This kit uses advanced anion-exchange silica membrane technology to selectively bind and purify plasmid DNA from lysed bacterial cells. The process includes lysing the cells, binding DNA to the column, washing away contaminants, and eluting pure DNA, typically within 35-45 minutes.

Q: What are the main benefits of using this kit compared to traditional plasmid purification methods?

A: The kit offers rapid processing, high yields (up to 1200 g per prep), and consistent DNA purity (A260/A280 ratio of 1.80-1.90). It's scalable and optimized for downstream applications such as transfection, sequencing, and cloning, making it more efficient and reliable than conventional protocols.

Q: When should I choose this kit for my DNA purification needs?

A: This kit is recommended when high-quality, large-scale plasmid DNA is needed for sensitive downstream applications. Its design particularly suits laboratories seeking expedited workflows and reproducible results from E. coli-based plasmid preps.

Q: Where can the QIAGEN Plasmid Plus Maxi Kit be used and stored?

A: The kit is intended for use in laboratory environments, suitable for academic, clinical, or research settings. All components should be stored at room temperature (15-25C) and remain stable for up to 12 months.

Q: What types and volumes of cultures are compatible with this kit?

A: The kit is compatible with E. coli cultures containing plasmids, processing culture volumes between 150 ml and 750 ml per prep. This makes it suitable for both standard laboratory and high-throughput workflows.

Q: What is the process for extracting plasmid DNA using this kit?

A: Extraction involves harvesting E. coli cells, lysing them with included buffers, binding the DNA to the provided Plasmid Plus Maxi columns, washing away impurities, and then eluting the purified DNA in 0.15-0.6 ml final volume. The entire procedure can be completed manually within 35-45 minutes.

Q: What downstream applications can benefit from plasmid DNA purified with this kit?

A: Plasmid DNA isolated using this kit is ideal for transfection, cloning, restriction digestion, PCR, and sequencing due to its high purity and yield, meeting the demands of modern molecular biology applications.

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