BioRad QX200 Droplet Digital PCR System

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BioRad QX200 Droplet Digital PCR System Price And Quantity

Product Description

Brand BioRad

Model QX200

Capacity 96 Wells

CAT No 1864001

Application Molecular Biology

The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.

Advanced Droplet Digital PCR Technology

The QX200 leverages droplet digital PCR (ddPCR) to partition each sample into approximately 20,000 droplets, enabling absolute quantification with exceptionally high sensitivity. Its two-channel fluorescence detection-compatible with FAM and HEX dyes-makes multiplex assays straightforward for diverse research and diagnostic applications.

Optimized Workflow and Flexibility

Supporting up to 96 samples per run and working with DNA, cDNA, and RNA (following RT steps), the QX200 is suitable for high-throughput environments. Integrated temperature control (4C-100C) ensures robust PCR protocols, and compatibility with 96-well plates and BioRad ddPCR reagents streamlines setup for consistent results.

Reliable Performance and Data Handling

With an internal memory of 16 GB, LCD display, and connectivity options including USB and Ethernet, the QX200 offers modern data transfer and streamlined analysis. QuantaSoft Analysis Pro software enhances user experience and delivers detailed insights, while the sturdy design maintains quiet operation and energy efficiency.

FAQ's of BioRad QX200 Droplet Digital PCR System:

Q: How does the QX200 Droplet Digital PCR System achieve precise quantification?

A: The system partitions the PCR reaction mix into up to 20,000 nanoliter-sized droplets, allowing absolute quantification of target nucleic acids. Each droplet acts as an individual PCR microreactor, and fluorescence detection provides highly accurate results down to one part per million (ppm) sensitivity.

Q: What types of samples can be processed on the QX200 system?

A: The QX200 supports DNA, cDNA, and RNA samples (with a required reverse transcription step for RNA). Its protocol flexibility makes it suitable for a wide range of applications, including gene expression studies, copy number variation, and pathogen detection.

Q: When is the best scenario to use this system in the lab?

A: The QX200 is ideal when you require highly sensitive and precise quantification, particularly for applications where traditional qPCR is limited by sensitivity or background noise. It excels in research and diagnostic workflows that demand multiplexing and absolute quantification.

Q: Where is the QX200 system typically installed and used?

A: This system is designed for benchtop use in molecular biology laboratories, clinical labs, and research institutions. Due to its size and weight, it is not wall-mounted or portable, and it operates under standard atmospheric pressure and humidity conditions.

Q: What is the workflow process for running a sample on the QX200?

A: First, samples are prepared with compatible BioRad ddPCR reagents and loaded into 96-well plates. The system generates droplets for each sample, then performs thermal cycling. After amplification, the droplets are read via fluorescence detection, and data are analyzed using QuantaSoft Analysis Pro software.

Q: What are the key benefits of using the QX200 over conventional PCR platforms?

A: Key benefits include higher sensitivity, superior precision, the ability to multiplex, and absolute target quantification without the need for standard curves. This enables robust detection of low-abundance targets and more confident data interpretation.

Q: How can results from the QX200 system be synchronized or exported for further analysis?

A: Results can be transferred using USB or Ethernet connections to PC-compatible computers, or shared with other instruments. The QuantaSoft Analysis Pro software facilitates data export and integration into downstream analytical platforms.

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