Qiagen QIAseq FastSelect Epidemiology Kit (96)

Qiagen QIAseq FastSelect Epidemiology Kit (96)

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Product Details:

Usage Type Laboratory Research Use Only
Function Removes unwanted ribosomal and viral RNA for improved RNA-Seq analysis
Accuracy High specificity for rRNA and viral RNA depletion
Storage Instructions Store at -20C upon receipt
Shelf Life 12 months (when stored as instructed)
Features Ultra-fast protocol; Compatible with Illumina platforms; Efficient depletion of rRNA and viral RNA from human, mouse, rat, and other epidemiological samples
Instruments Type Nucleic Acid Preparation Kit
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Qiagen QIAseq FastSelect Epidemiology Kit (96) Price And Quantity

Sample Input Required 10 ng1 g total RNA per reaction
Intended Use Epidemiological Screening, Outbreak Surveillance, Pathogen Identification, Viral Detection in RNA-Seq
Compatible Sample Types Total RNA from a wide variety of organisms including human, mouse, rat, and viral pathogens
Included Components FastSelect reagent mix, buffers, detailed protocol
Platform Compatibility Illumina Next-Generation Sequencing platforms
Kit Size 96 reactions
Protocol Time Approx. 10 minutes hands-on time
Format Pre-measured, ready-to-use reagents
Quality Control Stringent quality-tested reagents for reproducible results

Qiagen QIAseq FastSelect Epidemiology Kit (96) Product Specifications

Function Removes unwanted ribosomal and viral RNA for improved RNA-Seq analysis
Operating Type Manual
Instruments Type Nucleic Acid Preparation Kit
Power Source Not Required (Enzymatic/Chemical Reagents)
Accuracy High specificity for rRNA and viral RNA depletion
Storage Instructions Store at -20C upon receipt
Portable Yes
Weight Approx. 0.5 kg
Dimension (L*W*H) Box Size: Approximately 20 x 15 x 8 cm
Equipment Type Epidemiology Library Preparation Kit
Features Ultra-fast protocol; Compatible with Illumina platforms; Efficient depletion of rRNA and viral RNA from human, mouse, rat, and other epidemiological samples
Material Molecular Biology Grade Reagents and Enzymes
Real-Time Operation No
Usage Type Laboratory Research Use Only
Condition New
Technology FastSelect rRNA and Viral RNA Depletion
Color White/Colorless (Reagents in Vials)
Wall Mounted No
Use For Preparing RNA-Seq Libraries by Depleting Host and Virus rRNA/RNA from Epidemiology Samples
Shelf Life 12 months (when stored as instructed)

Sample Input Required 10 ng1 g total RNA per reaction
Intended Use Epidemiological Screening, Outbreak Surveillance, Pathogen Identification, Viral Detection in RNA-Seq
Compatible Sample Types Total RNA from a wide variety of organisms including human, mouse, rat, and viral pathogens
Included Components FastSelect reagent mix, buffers, detailed protocol
Platform Compatibility Illumina Next-Generation Sequencing platforms
Kit Size 96 reactions
Protocol Time Approx. 10 minutes hands-on time
Format Pre-measured, ready-to-use reagents
Quality Control Stringent quality-tested reagents for reproducible results

Product Description

Brand Qiagen

Product Type PCR Kit

Pack Size 96 Wells

CAT No 333275

Application Molecular Reagent

QIAseq FastSelect Epidemiology Kits are designed for "dual-seq or "host-pathogen RNA-seq Library construction. Researchers using QIAseq FastSelect Epidemiology Kits for RNA-seq library construction will remove bacterial 5S/15S/23S rRNA and human, mouse and rat cytoplasmic and mitochondrial ribosomal RNA. The reagents for bacterial and HMR rRNA removal are supplied in separate tubes, allowing researchers to optimize their RNA-seq workflows for dual-seq, bacteria or HMR samples.

Rapid and Reliable RNA Depletion for Epidemiological Research

Harnessing FastSelect technology, this kit simplifies the removal of unwanted ribosomal and viral RNA, streamlining RNA-Seq library preparation for accurate epidemiological studies. Benefit from a hands-on protocol of just 10 minutes, reducing sample processing time while enhancing detection of critical targets, making it ideal for outbreak response and routine surveillance.

Broad Sample Compatibility and Robust Results

Compatible with total RNA from various organisms, including human, mouse, rat, and diverse viral pathogens, this kit supports research across a wide range of epidemiological inquiries. Stringently tested reagents provide confidence in both sensitivity and specificity, facilitating high-quality data for pathogen identification and public health investigations.

FAQ's of Qiagen QIAseq FastSelect Epidemiology Kit (96):

Q: How does the QIAseq FastSelect Epidemiology Kit improve RNA-Seq analysis?

A: The kit efficiently depletes ribosomal and viral RNA from total RNA samples, allowing greater sequencing depth for target pathogen identification and reducing background noise. This selective removal enhances the accuracy and informativeness of RNA-Seq data, particularly important in epidemiological and outbreak settings.

Q: What types of RNA samples are compatible with this kit?

A: This kit is designed for use with total RNA from a variety of organisms including humans, mice, rats, and viral pathogens, making it versatile for numerous epidemiology research applications.

Q: When should I use this kit in my workflow?

A: Use the QIAseq FastSelect Epidemiology Kit during the RNA-Seq library preparation process, specifically after RNA extraction and before cDNA synthesis, to remove unwanted rRNA and viral RNA and improve sequencing results.

Q: Where can this kit be utilized?

A: This kit is well-suited for laboratory research environments focused on epidemiological screening, outbreak surveillance, and pathogen detection. It supports projects conducted by academic, clinical, and public health laboratories.

Q: What is the process for using the kit?

A: Begin by extracting total RNA, mix the RNA sample with the ready-to-use FastSelect reagents and buffers, and follow the detailed protocol provided. The protocol requires approximately 10 minutes of hands-on time, and the reagents efficiently deplete unwanted RNA species before library construction.

Q: What are the benefits of using this kit for epidemiological studies?

A: By increasing the proportion of informative RNA in your samples, the kit boosts the sensitivity and accuracy of viral and pathogen detection. Its ultra-fast workflow and high specificity offer rapid, reproducible results vital for timely outbreak surveillance and research.

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