Product Description
Brand InBios
Sample Type Serum
Pack Size 96 Wells
CAT No ZKM2-1
The ZIKV Detect 2.0 IgM Capture ELISA is intended for the qualitative detection of Zika virus IgM antibodies in human sera for the presumptive clinical laboratory diagnosis of Zika virus infection. The assay is intended for use only in patients with clinical signs and symptoms consistent with Zika virus infection, and/or CDC Zika virus epidemiological criteria (e.g., history of residence in or travel to a geographic region with active Zika transmission at the time of travel, or other epidemiological criteria for which Zika virus testing may be indicated). Assay results are for the presumptive detection of IgM antibodies to Zika virus (ZIKV). Positive results must be confirmed by following the latest CDC guidelines for the diagnosis of Zika virus infection.
Reliable Zika Virus IgM DetectionThis kit offers high accuracy in detecting IgM antibodies against the Zika Virus in human serum. Its capture ELISA format ensures robust detection at validated cut-off levels, minimizing cross-reactivity and maximizing specificity. Researchers benefit from ready-to-use components and clear documentation.
Fast and Efficient WorkflowWith a total assay time of less than 2.5 hours and streamlined steps, the kit fits well into busy laboratory schedules. Each kit includes reagents and controls required to process up to 96 samples, making high-throughput screening straightforward and timely.
User-Friendly and FlexibleDesigned for manual operation and requiring only an ELISA reader, the kit is straightforward to implement in various research laboratory environments. Storage is convenient at 2-8C, and the kit's standard dimensions make it easy to transport and store as needed.
FAQ's of InBios ZIKV Detect 2.0 IgM Capture ELISA Kit:
Q: How does the InBios ZIKV Detect 2.0 IgM Capture ELISA Kit work?
A: The kit utilizes a capture ELISA method to detect IgM antibodies specific to Zika Virus in human serum. IgM antibodies in the sample are captured by plate-bound anti-IgM, followed by detection with conjugated antigen, substrate reaction, and absorbance measurement at 450 nm.
Q: What type of samples can be tested with this kit?
A: This kit is validated for use with human serum samples only. Serum should be collected, processed, and stored according to standard laboratory protocols before analysis.
Q: When should I use this kit during the course of infection?
A: The kit is intended for use with samples collected during the acute phase when IgM antibodies are present, typically within the first few days to weeks after infection onset. Consult current guidelines for optimal sample collection times.
Q: Where can the InBios ZIKV Detect 2.0 IgM ELISA kit be used?
A: It is suitable for use in research laboratory settings equipped with standard microplate ELISA readers. The kit is intended for Research Use Only (RUO) and not for diagnostic purposes.
Q: What is the assay process and required equipment?
A: Add 100 L of human serum to each well, follow the kit instructions to add reagents and controls, incubate, wash, and add substrate. Read the absorbance at 450 nm using a compatible ELISA plate reader. The full protocol is provided in the kit's manual.
Q: What are the benefits of using this kit?
A: Researchers benefit from fast results, high sensitivity and specificity, ready-to-use reagents, and quality controls included in the kit. The kit enables qualitative detection of ZIKV IgM antibodies, aiding research into Zika infection dynamics.
Q: How should the kit be stored and how long does it last?
A: The kit should be stored at 2-8C and remains stable for up to 12 months from the date of manufacture, as specified by the manufacturer's instructions.