Thermo Fisher Scientific Invitrogen PureLink PCR Micro Kit

Thermo Fisher Scientific Invitrogen PureLink PCR Micro Kit

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Product Details:

Instruments Type Molecular Biology Kit
Shelf Life 1 year from date of manufacture
Function Extraction and purification of DNA from PCR reactions
Measurement Range Purifies fragments from 100 bp to 12 kb
Features Efficient DNA recovery, rapid protocol, compatible with micro-scale samples
Accuracy Up to 95% recovery of DNA fragments
Usage Type Laboratory/Research use (for in vitro studies only)
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Thermo Fisher Scientific Invitrogen PureLink PCR Micro Kit Price And Quantity

Thermo Fisher Scientific Invitrogen PureLink PCR Micro Kit Product Specifications

Condition New
Material High-quality reagent grade chemicals, silica-based membrane
Use For purification of PCR products (DNA) from enzymes, primers, and dNTPs
Function Extraction and purification of DNA from PCR reactions
Shelf Life 1 year from date of manufacture
Weight Not Applicable (Kit format)
Accuracy Up to 95% recovery of DNA fragments
Instruments Type Molecular Biology Kit
Real-Time Operation No
Equipment Type PCR Purification Kit
Portable Yes
Storage Instructions Store at room temperature (1530C)
Dimension (L*W*H) Not Applicable (Kit format)
Wall Mounted No
Features Efficient DNA recovery, rapid protocol, compatible with micro-scale samples
Technology Silica membrane spin column technology
Color White (columns) / Multi-color labeling for kit components
Measurement Range Purifies fragments from 100 bp to 12 kb
Operating Type Manual
Usage Type Laboratory/Research use (for in vitro studies only)

DNA Binding Capacity Up to 5 g DNA per column
Processing Time As little as 5 minutes per sample
Kit Format 50 reactions per kit
Sample Input Volume 15 l PCR reaction
Compatible Buffers Supplied with all required buffers
Elution Volume 1020 l
Applications PCR product clean-up, Sanger sequencing, cloning, restriction analysis
Product Code K310250

Product Description

Brand	Thermo Fisher Scientific
Product Type	PCR Micro Kit
Pack Size	250 Preps
CAT No	K310250
Product Class	Molecular Biology

PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions. These highly purified DNA fragments and amplicons provide excellent results in downstream applications such as DNA sequencing, restriction digestion, cloning, PCR, and labeling. PureLink PCR Micro Kits provide a simple and rapid method for purifying and concentrating DNA fragments ranging in size from 100 bp-12 kb.

Efficient PCR Product Purification

The PureLink PCR Micro Kit ensures highly efficient DNA purification, delivering up to 95% recovery of DNA fragments from 100 bp to 12 kb. Its rapid protocol allows researchers to process samples in a minimal time frame, making it suitable for high-throughput labs and time-sensitive experiments.

Silica Membrane Spin Column Technology

Harnessing advanced silica membrane technology, the kit provides exceptional binding capacity and specificity, capturing up to 5 g DNA per column. The color-coded components and straightforward procedure make the purification process reliable and easy to follow.

Designed for Versatility and Reliability

Suitable for a variety of applications including Sanger sequencing, cloning, and restriction analysis, this kit meets the needs of researchers in genomics, molecular biology, and diagnostics. Its reagent-grade materials ensure consistent performance over its one-year shelf life.

FAQ's of Thermo Fisher Scientific Invitrogen PureLink PCR Micro Kit:

Q: How do I use the Invitrogen PureLink PCR Micro Kit for DNA purification?

A: To purify DNA from PCR products, add your sample (1-5 l PCR reaction) and binding buffer to the spin column, centrifuge, wash with the supplied buffers, and elute in 10-20 l buffer. The process typically takes as little as 5 minutes per sample.

Q: What types of DNA fragments can be purified using this kit?

A: The PureLink PCR Micro Kit is suitable for purifying DNA fragments ranging from 100 base pairs to 12 kilobases in size, making it ideal for most PCR and molecular cloning applications.

Q: When should I use this kit in my workflow?

A: You should use the PureLink PCR Micro Kit immediately after PCR amplification to remove enzymes, primers, and dNTPs before downstream processes such as sequencing, cloning, or restriction digestion.

Q: Where should I store the PureLink PCR Micro Kit?

A: Store the kit at room temperature (15-30C) as recommended. This ensures the longevity of reagent-grade chemicals and membranes, preserving optimal performance throughout the one-year shelf life.

Q: What are the benefits of using the PureLink PCR Micro Kit over other purification methods?

A: This kit offers rapid processing, high recovery rates (up to 95%), compatibility with small sample volumes, and simple, manual operation without the need for specialized equipment, enhancing efficiency and reliability in molecular biology workflows.

Q: Is specialized equipment required for operation?

A: No specialized equipment is needed apart from a standard benchtop centrifuge. The kit is entirely manual, making it easily adaptable to typical laboratory setups.

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