Product Description
| Brand |
Thermo Fisher Scientific |
| Product Type |
RNase Control Reagent |
| Pack Size |
25 Reactions |
| CAT No |
4479768 |
| Product Class |
Molecular Biology |
RNaseAlert Lab Test Kit v2 detects RNase activity in a convenient and sensitive fluorimetric assay that delivers results in real time. This kit is suitable for testing small numbers of sample and ensuring that solutions, tubes, tips, etc. in your lab are RNase-free. This new version contains a more sensitive substrate that is a1460% more sensitive than the previous version. The kit contains sufficient reagents for 25 reactions.
Fast and Sensitive RNase DetectionThe RNaseAlert Lab Test Kit v2 delivers quick and reliable results, detecting as little as 1x10^-9 units of RNase A within 30 minutes. Its fluorometric readout enables real-time observation, making it invaluable for labs that require stringent RNase-free conditions.
Versatile Sample CompatibilityThis kit is suitable for testing RNase contamination in water, buffers, laboratory solutions, and on plasticware surfaces. Its wide compatibility enhances workflow flexibility, allowing for comprehensive monitoring across various laboratory applications.
Easy-to-Use, No Equipment RequiredAll necessary reagents-including synthetic RNA substrate, fluorescent dye, reaction buffer, and control reagents-are provided. Manual operation ensures no dependency on electrical power, and results can be read using any compatible fluorescence microplate reader or fluorometer.
FAQ's of Thermo Fisher Scientific Invitrogen RNaseAlert Lab Test Kit v2:
Q: How does the RNaseAlert Lab Test Kit v2 detect RNase contamination?
A: The kit uses a synthetic RNA substrate labeled with a fluorescent dye and quencher. When RNase is present, the substrate is cleaved, releasing fluorescence that can be measured with a compatible fluorometer at excitation/emission wavelengths of 490 nm/520 nm.
Q: What is the process for using the RNaseAlert Lab Test Kit v2?
A: Simply combine your samples with the provided reagents following the user manual, incubate for as little as 30 minutes, and measure the fluorescence increase using a microplate reader or fluorometer to determine RNase activity.
Q: Where can this kit be used?
A: The kit is intended for research and diagnostic laboratories, especially those focused on molecular biology or quality assurance, and is suitable for use in India and other regions supplied by authorized dealers and distributors.
Q: What types of samples can I test with this kit?
A: You can detect RNase activity in water, laboratory buffers, various solutions, and on surfaces of laboratory plasticware, ensuring comprehensive contamination monitoring.
Q: When should I use the RNaseAlert Lab Test Kit v2 in my workflow?
A: This kit should be used whenever you need to verify RNase-free conditions, particularly prior to sensitive RNA work, or for periodic quality checks for potential RNase contamination in laboratory environments.
Q: What are the benefits of using this RNase detection kit?
A: Benefits include fast and sensitive detection, wide dynamic measurement range, compatibility with varied workflows, and no requirement for electrical power. It ensures data integrity by confirming RNase-free surfaces and reagents.
Q: How should I store the kit components for maximum shelf life?
A: Store all kit reagents at -20C to maintain optimal performance and ensure the shelf life of up to 12 months from the date of manufacture.