Qiagen Taq DNA Polymerase (25000)

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Product Details:

Instruments Type PCR reagent
Measurement Range Up to 5 kb (kilobases) DNA fragments
Accuracy High fidelity for standard Taq polymerase
Features High specificity, robust DNA amplification, supplied with optimized buffer
Usage Type Laboratory / Research use only
Storage Instructions Store at -20C
Function Catalyzes DNA synthesis during PCR
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Qiagen Taq DNA Polymerase (25000) Price And Quantity

Qiagen Taq DNA Polymerase (25000) Product Specifications

Accuracy High fidelity for standard Taq polymerase
Material Recombinant Taq DNA Polymerase in storage buffer
Equipment Type Enzyme (Taq DNA Polymerase)
Instruments Type PCR reagent
Technology PCR (Polymerase Chain Reaction)
Measurement Range Up to 5 kb (kilobases) DNA fragments
Use For routine and specialized PCR applications
Portable Yes (Enzyme supplied in vials/packaging)
Wall Mounted No
Color Colorless to slight yellow (buffer); clear solution
Operating Type Manual
Condition New
Shelf Life 12 months (when stored under recommended conditions)
Storage Instructions Store at -20C
Usage Type Laboratory / Research use only
Real-Time Operation No (Standard PCR)
Features High specificity, robust DNA amplification, supplied with optimized buffer
Function Catalyzes DNA synthesis during PCR

Product Description

Brand Qiagen

Product Type PCR Kits

Pack Size 2500 ul

CAT No 203209

Application Molecular Reagent

HotStarTaq DNA Polymerase uses a chemically mediated hot start that, unlike, antibody-mediated systems, leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. HotStarTaq DNA Polymerase is supplied with the unique QIAGEN PCR Buffer, which minimizes nonspecific amplification products, primer dimers, and background. Q-Solution, a novel additive that enables efficient amplification of "difficult" (e.g., GC rich) templates, is also provided.

Advanced Formulation for Reliable PCR

This Taq DNA polymerase is optimized for consistent performance in a wide range of PCR applications, from cloning to diagnostics. The enzyme's buffer system enhances specificity and reaction robustness, allowing high-fidelity amplification across diverse sample types and conditions.

Flexible and Easy-to-Use Enzyme

Qiagen Taq DNA polymerase can be used with all standard thermal cyclers and integrates into most common PCR protocols. Supplied in convenient vials, the enzyme is portable and straightforward to store at -20C, ensuring flexibility for laboratory workflows.

Designed for Research Needs

With its capacity for amplifying DNA fragments up to 5 kb, this polymerase is ideal for standard and specialized research tasks. The 25,000-unit pack is suited for users requiring a reliable, high-volume supply for frequent or large-scale PCR experiments.

FAQ's of Qiagen Taq DNA Polymerase (25000):

Q: How do I set up a typical PCR reaction using Qiagen Taq DNA Polymerase?

A: To set up a standard 50 l PCR reaction, use 1-2.5 units of Qiagen Taq DNA Polymerase. Combine the enzyme with the recommended buffer (which contains MgCl2, KCl, Tris-HCl, and stabilizers), dNTPs, primers, template DNA, and water. Mix gently and run the reaction in your thermal cycler as per your cycling protocol.

Q: What is the shelf life and storage requirement for this polymerase?

A: The shelf life of Qiagen Taq DNA Polymerase is 12 months when stored at -20C. Always return the enzyme to the freezer immediately after use to maintain stability and activity. Do not thaw and refreeze repeatedly.

Q: Where can I find the expiry date for my enzyme lot?

A: The expiry date for your batch is provided in the lot-specific Certificate of Analysis, which accompanies each product shipment. Ensure to reference this document before use for quality assurance.

Q: What are the benefits of using Qiagen Taq DNA Polymerase for my applications?

A: This enzyme offers robust DNA amplification with high specificity, making it suitable for cloning, genotyping, mutagenesis, routine PCR, and diagnostic assays. Its optimized buffer ensures consistent results across various DNA templates and workflows.

Q: Can Qiagen Taq DNA Polymerase be used with any thermal cycler?

A: Yes, this polymerase is compatible with all major thermal cyclers, allowing seamless integration into virtually any laboratory PCR setup.

Q: What is the maximum fragment size I can amplify with this enzyme?

A: Qiagen Taq DNA Polymerase reliably amplifies DNA fragments up to 5 kb, making it suitable for most standard PCR applications in research laboratories.

Q: In what format and condition is the enzyme shipped?

A: The enzyme is supplied as a colorless to slight yellow clear solution in vials, shipped securely on dry ice to ensure it remains stable and active during transport.

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