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Qiagen QuantiFast Multiplex PCR Kit (400) Qiagen QuantiFast Multiplex PCR Kit (400) Qiagen QuantiFast Multiplex PCR Kit (400)
Qiagen QuantiFast Multiplex PCR Kit (400)
Qiagen QuantiFast Multiplex PCR Kit (400) Qiagen QuantiFast Multiplex PCR Kit (400)

Qiagen QuantiFast Multiplex PCR Kit (400)

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Product Details:

  • Instruments Type Consumable reagent kit for PCR
  • Features High specificity, fast cycling, robust multiplex PCR, hot-start DNA polymerase
  • Usage Type Laboratory reagent
  • Function Simultaneous amplification and quantification of multiple DNA targets
  • Shelf Life 12 months if stored properly
  • Measurement Range Dependent on primer and probe design and template
  • Storage Instructions Store at -20C; avoid repeated freeze-thaw cycles
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Qiagen QuantiFast Multiplex PCR Kit (400) Price And Quantity

  • Fast cycling protocols; typically 60 min or less
  • DNA and cDNA
  • 2x QuantiFast Multiplex PCR Master Mix, Q-Solution, RNase-Free Water, Reference Dye (if required)
  • All standard and real-time PCR cyclers
  • Refer to Material Safety Data Sheet (MSDS)
  • Boxed Kit
  • Multiplex PCR, gene expression analysis, pathogen detection
  • 400 reactions

Qiagen QuantiFast Multiplex PCR Kit (400) Product Specifications

  • Simultaneous amplification and quantification of multiple DNA targets
  • Consumable reagent kit for PCR
  • ~500 g (gross packaging weight)
  • For multiplex quantitative real-time PCR detection
  • Reagent chemicals / laboratory grade plastic (consumable vials, tubes)
  • Manual, for use with automated thermal cyclers
  • New
  • Dependent on primer and probe design and template
  • 12 months if stored properly
  • Not Applicable (used with separate thermal cycler)
  • Kit packaging: Standard manufacturer box size
  • No
  • Laboratory reagent
  • Store at -20C; avoid repeated freeze-thaw cycles
  • PCR Kit
  • Yes
  • High specificity, fast cycling, robust multiplex PCR, hot-start DNA polymerase
  • Multiplex Real-Time PCR
  • Yes (as a kit)
  • High; suitable for quantitative applications
  • Reagent vials are colorless/clear; box is typically white with Qiagen branding
  • Fast cycling protocols; typically 60 min or less
  • DNA and cDNA
  • 2x QuantiFast Multiplex PCR Master Mix, Q-Solution, RNase-Free Water, Reference Dye (if required)
  • All standard and real-time PCR cyclers
  • Refer to Material Safety Data Sheet (MSDS)
  • Boxed Kit
  • Multiplex PCR, gene expression analysis, pathogen detection
  • 400 reactions

Product Description

Brand                       Qiagen

Product Type           PCR Kit

Pack Size                 400T

CAT No                     204654

Application              Molecular Reagent

QuantiFast Multiplex PCR Kits enable fast and reliable quantification of up to 4 cDNA or gDNA targets in a single tube by multiplex, real-time PCR or two-step RT-PCR. Q-bond technology and an optimized master mix promote fast multiplex real-time PCR, not only on fast cyclers with short ramping times, but also on standard cyclers. The combination of a hot start and a unique PCR buffer system in the ready-to-use master mix ensures highly sensitive qPCR on any real-time cycler without the need for optimization. Two kit formats are available: the QuantiFast Multiplex PCR Kit for cyclers that require ROX dye for fluorescence normalization, and the QuantiFast Multiplex PCR +R Kit for all other cyclers. For convenience, the master mix can be stored at 28C.



Accelerate Multiplex PCR Performance

Optimize your workflow with rapid, simultaneous detection of multiple targets using the QuantiFast Multiplex PCR Kit. Engineered for reliable, fast cycling (under 60 minutes), it facilitates gene expression studies and pathogen identification with robust accuracy and specificity. The hot-start DNA polymerase ensures precise quantification, reducing the risk of non-specific amplifications.


Comprehensive Compatibility and Easy Integration

This kit seamlessly integrates with all standard and real-time PCR instruments, enabling versatile usage across platforms. It features reagents and consumables housed in laboratory-grade plastic, contained in a convenient Qiagen-branded box. As a manual, portable kit, it fits a variety of laboratory workflows and infrastructure.


Safe Storage and Reliable Shelf Life

Maintain reagent integrity by storing the kit at -20C and avoiding repeated freeze-thaw cycles. With proper storage, expect a shelf life of up to 12 months, ensuring consistent, high-quality results for your multiplex PCR applications.

FAQs of Qiagen QuantiFast Multiplex PCR Kit (400):


Q: How do I use the Qiagen QuantiFast Multiplex PCR Kit for my experiments?

A: To use the kit, prepare your PCR reactions with the included 2x Multiplex PCR Master Mix, Q-Solution, and RNase-free water, following the instructions for your sample type (DNA or cDNA) and compatible real-time PCR cycler. Add your primers and probes, and reference dye if needed, then follow the suggested thermal cycling protocol.

Q: What applications is this kit suitable for?

A: This kit is designed for multiplex PCR, enabling gene expression analysis and pathogen detection by targeting multiple DNA sequences in a single reaction. It is highly accurate for quantitative assays requiring high specificity and sensitivity.

Q: When should I use the reference dye included in the kit?

A: The reference dye is used for normalization in real-time PCR, depending on your instruments requirements. Refer to your cyclers guidelines to determine if it is necessary for your experiment setup.

Q: Where can the Qiagen QuantiFast Multiplex PCR Kit be used?

A: This kit is intended for research laboratories equipped with standard or real-time PCR cyclers and is suitable for academic, clinical, or industrial research settings where multiplex quantitative PCR is required.

Q: What is the process for preparing and storing the kit reagents?

A: Upon receipt, store the kit at -20C. When preparing reactions, thaw only the needed reagents and avoid repeated freeze-thaw cycles to maintain reagent stability and extend shelf life.

Q: What are the main benefits of using this multiplex PCR kit?

A: The primary benefits are rapid reaction time (under 60 minutes), robust multi-target amplification, high specificity, excellent quantitative accuracy, and compatibility with a wide range of PCR instruments, making it versatile and reliable for various research applications.

Q: How long is the shelf life and what are the storage requirements?

A: The kit maintains optimal performance for up to 12 months when stored at -20C and protected from frequent freeze-thaw cycles, ensuring consistent results throughout its intended use period.

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