Product Description
Brand InBios
Sample Type Serum
Pack Size 96 Wells
CAT No JEMS-1
The JE Detect IgM Antibody Capture ELISA (MAC-ELISA) Kit for exposure to Japanese Encephalitis Virus (JEV) is an ELISA assay system for the detection of IgM antibodies in human serum to JEV-derived recombinant antigen.
Rapid and Reliable Detection of JE IgMInBios JE Detect offers a trusted solution for laboratories needing accurate detection of IgM antibodies to Japanese Encephalitis virus. The 96-well format allows high-throughput testing-up to 96 samples per plate-making it suitable for both individual diagnoses and larger epidemiological studies. The kit's ready-to-use reagents and straightforward manual protocol streamline workflow and minimize errors.
Comprehensive Kit Design for Ease of UseThis ELISA kit provides all required components, including a coated microplate, sample diluent, wash buffer, conjugate, substrate, stop solution, as well as positive and negative controls. Clear, detailed instructions support the consistent execution of every assay. Its robust design ensures reliable, reproducible results when operated by skilled laboratory professionals.
High Sensitivity and SpecificityThe JE Detect ELISA is engineered for high sensitivity and specificity toward JE virus IgM antibodies, resulting in minimal cross-reactivity with other pathogens. The qualitative result is easily interpreted using a standard ELISA reader at 450 nm. Each kit's performance parameters can be referenced in the included instructions for use (IFU).
FAQ's of InBios JE Detect IgM Antibody Capture ELISA:
Q: How does the JE Detect IgM ELISA identify Japanese Encephalitis virus infection?
A: The JE Detect IgM ELISA captures IgM antibodies in patient serum or CSF using an anti-human IgM-coated microplate. Following introduction of a JE antigen conjugate and subsequent incubation with colorimetric reagents, a color change occurs in the presence of JE virus-specific IgM. Results are read at 450 nm using an ELISA reader.
Q: What sample types are appropriate for this assay, and how should they be prepared?
A: This kit is validated for use with human serum or cerebrospinal fluid (CSF). Samples should be collected and prepared following standard laboratory protocols. Be sure to avoid hemolysis and contamination to ensure accurate results.
Q: When can I expect test results, and how many samples can be run per plate?
A: The complete assay requires approximately 2 to 2.5 hours. Each 96-well microplate enables the testing of up to 96 samples, making the kit suitable for high-throughput laboratory settings.
Q: Where should the JE Detect IgM ELISA kit be stored, and for how long can it be used?
A: Store the kit at 2-8C and never freeze the reagents. Under these conditions, the kit remains stable for 12 months from the manufacturing date. Always refer to the kit insert for additional storage guidance.
Q: What controls are included, and why are they important?
A: The kit includes both positive and negative controls. These controls are essential to verify assay validity and ensure accurate interpretation of patient results in every run.
Q: What are the main benefits of using this ELISA kit for JE diagnosis?
A: Key benefits include its high specificity and sensitivity for JE virus IgM, rapid turnaround time, and user-friendly format with ready-to-use reagents. This enables dependable and efficient JE diagnosis in clinical laboratories.