Qiagen QIAamp DNA Mini Kit (250)

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Product Details:

Features High-purity DNA yield, ready-to-use spin columns, rapid and easy protocol, suitable for multiple downstream applications
Storage Instructions Store at room temperature
Instruments Type Nucleic acid purification kit
Shelf Life 12 months
Usage Type Laboratory
Function Extraction and purification of genomic, mitochondrial, bacterial, viral, and fungal DNA
Measurement Range Sample volume typically 200 l
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Qiagen QIAamp DNA Mini Kit (250) Price And Quantity

Qiagen QIAamp DNA Mini Kit (250) Product Specifications

Instruments Type Nucleic acid purification kit
Real-Time Operation No
Use Isolation and purification of DNA from various sample types
Shelf Life 12 months
Weight Variable, typically approx. 23 kg per kit
Equipment Type DNA Extraction Kit
Technology Silica membrane technology
Operating Type Manual (spin column-based)
Accuracy High DNA purity and consistency
Features High-purity DNA yield, ready-to-use spin columns, rapid and easy protocol, suitable for multiple downstream applications
Dimension (L*W*H) Standard kit box size (manufacturers packaging)
Material High-quality plastic components, pre-filled buffers, and silica-based membranes
Storage Instructions Store at room temperature
Usage Type Laboratory
Function Extraction and purification of genomic, mitochondrial, bacterial, viral, and fungal DNA
Condition New
Wall Mounted No
Portable Yes
Color White/transparent (columns, tubes), colored labels
Power Source Not required (manual centrifugation)
Measurement Range Sample volume typically 200 l

Product Description

Brand	Qiagen
Product Type	DNA Extraction
Pack Size	250 T
CAT No	51306
Application	Molecular Biology

The QIAamp DNA Micro Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 µL. DNA purified using the QIAamp DNA Micro Kit is free of proteins, nucleases and other impurities, and is suitable for use in sensitive downstream applications, such as real-time PCR. Purified DNA may also be used in short-tandem repeat (STR) genotyping, single-nucleotide polymorphism (SNP) genotyping or pharmacogenomic research.

Streamlined DNA Extraction Process

Benefit from a rapid and user-friendly protocol that allows for DNA isolation in just 20-40 minutes per sample. The pre-filled buffers and ready-to-use spin columns facilitate consistent, high-purity yields across a range of sample types such as blood, tissues, and swabs. The kit's silica membrane technology ensures reproducible results in your lab workflow.

Reliable for Multiple Sample Types

The QIAamp DNA Mini Kit is compatible with numerous biological specimens, making it extremely versatile. Whether working with human, animal, or microbial samples, this kit provides reliable extraction suitable for critical downstream analyses such as PCR, sequencing, and genotyping. Its robust performance is trusted by laboratories worldwide.

Safe and Convenient Laboratory Use

Contain no hazardous chemicals, the kit is designed for standard laboratory handling and room temperature storage. The portable, compact box contains all required components, reducing the need for additional reagents or equipment. Shelf life extends up to 12 months, ensuring long-term reliability.

FAQ's of Qiagen QIAamp DNA Mini Kit (250):

Q: How does the QIAamp DNA Mini Kit purify DNA from various sample types?

A: The kit uses silica membrane technology within spin columns to selectively bind DNA during centrifugation, enabling the removal of contaminants through a series of proprietary lysis, wash, and elution buffers. This process yields high-purity DNA ready for downstream applications.

Q: What types of samples can be processed with this kit?

A: It is optimized for extracting DNA from whole blood, tissues, body fluids, swabs, and a variety of other sample types. This versatility is ideal for both clinical and research settings.

Q: When should I choose this kit for my DNA extraction workflow?

A: Select this kit when you require consistent, high-yield DNA extraction across diverse sample matrices. It is particularly suited for applications needing pure DNA, including PCR, qPCR, sequencing, and genotyping.

Q: Where can the QIAamp DNA Mini Kit be safely used and stored?

A: The kit is intended for laboratory use. It is safe for standard lab handling as it contains no hazardous chemicals, and should be stored at room temperature according to the manufacturer's instructions.

Q: What is the typical process and time required per extraction?

A: Each DNA extraction takes approximately 20-40 minutes per sample. The procedure involves cell lysis with buffer and proteinase K, DNA binding to the column, multiple wash steps, and final DNA elution-all performed manually using a standard microcentrifuge.

Q: What are the primary benefits of using the QIAamp DNA Mini Kit?

A: Key benefits include high DNA recovery and purity, compatibility with various samples, reliable results for molecular biology assays, ease of use, and the absence of dangerous chemicals.

Q: Can the kit be used without specialized laboratory equipment?

A: Yes, it only requires a standard microcentrifuge. All other essential reagents and consumables needed for DNA extraction are provided in the kit.

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