Qiagen TurboCapture 96 mRNA Kit (5)

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Product Details:

Shelf Life As per manufacturer instructions, typically 12 months
Accuracy High mRNA purity for sensitive downstream applications
Storage Instructions Store at 28C
Features Fast mRNA purification, 96-well plate format, automation compatible, high yield and purity
Measurement Range Suitable for a wide range of sample input (as per protocol)
Instruments Type Nucleic acid purification kit
Function Isolation and purification of mRNA
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Qiagen TurboCapture 96 mRNA Kit (5) Price And Quantity

Qiagen TurboCapture 96 mRNA Kit (5) Product Specifications

Usage Type Laboratory use only
Function Isolation and purification of mRNA
Operating Type Manual or semi-automated with compatible platforms
Instruments Type Nucleic acid purification kit
Technology Magnetic bead-based mRNA purification
Condition New
Color White/transparent (kit components)
Measurement Range Suitable for a wide range of sample input (as per protocol)
Power Source Not required for kit (compatible with automation platforms if desired)
Features Fast mRNA purification, 96-well plate format, automation compatible, high yield and purity
Portable No
Storage Instructions Store at 28C
Equipment Type mRNA Extraction Kit
Accuracy High mRNA purity for sensitive downstream applications
Use High-throughput purification and isolation of mRNA from cell and tissue lysates
Real-Time Operation No
Shelf Life As per manufacturer instructions, typically 12 months
Wall Mounted No
Material High-quality laboratory-grade reagents and plastics

Product Description

Brand Qiagen

Product Type mRNA Kit

Pack Size 5 x 96 Wells

CAT No 72251

Application Molecular Reagent

The TurboCapture System uses unique oligonucleotide immobilization technology to provide a fast and simple procedure for high-throughput mRNA extraction. Cell lysates are added to the wells of a TurboCapture plate (96 or 384 wells), and mRNA is allowed to hybridize to the immobilized oligo-dT in each well. Contaminants are washed away, and the isolated mRNA is then either used directly in cDNA synthesis or eluted for use in downstream applications.

Efficient High-Throughput mRNA Purification

Designed for research labs with demanding sample volumes, the TurboCapture 96 mRNA Kit (5) streamlines mRNA isolation from up to 480 samples per kit. It integrates a fast purification protocol (under 90 minutes for 96 samples) with magnetic bead-based technology, ensuring consistency and high purity suitable for sensitive downstream applications.

Automation Ready and Flexible Workflows

The kit is compatible with both manual handling and semi-automated platforms like Qiagen BioRobot, adapting seamlessly to your laboratory's throughput needs. Its 96-well plate format simplifies integration into existing automation infrastructure, allowing users to boost efficiency without sacrificing performance or reliability.

Reliable Results for Diverse Applications

Whether your research requires gene expression studies, RT-PCR, qPCR, microarray analysis, or NGS, the TurboCapture 96 mRNA Kit (5) provides the mRNA purity and yield essential for accuracy. The inclusion of DNase I ensures genomic DNA is thoroughly removed, supporting trustworthy data in even the most demanding applications.

FAQ's of Qiagen TurboCapture 96 mRNA Kit (5):

Q: How is mRNA purified using the TurboCapture 96 mRNA Kit (5)?

A: The kit utilizes a magnetic bead-based technology within a 96-well plate format. Simply add sample lysates, and magnetic beads capture mRNA, followed by a washing and elution process, providing high-yield, pure mRNA suitable for downstream applications.

Q: What sample types are compatible with this kit?

A: The kit is optimized for extraction from cell lysates, tissue lysates, and a variety of other biological samples, making it versatile for numerous laboratory workflows.

Q: When should I use this kit for my experiments?

A: Use the TurboCapture 96 mRNA Kit (5) whenever you require fast, reliable, and high-throughput purification of mRNA for studies like gene expression analysis, RT-PCR, qPCR, microarrays, or next-generation sequencing.

Q: Where can the kit be operated-manually or with automation?

A: You can process samples either manually or with automated platforms such as the Qiagen BioRobot. The kit's design ensures flexibility for laboratory preferences and infrastructure.

Q: What is the typical processing time for 96 samples?

A: The complete workflow for purifying mRNA from 96 samples can be finished in less than 90 minutes, facilitating efficient high-throughput sample processing.

Q: How does the inclusion of DNase I benefit the process?

A: DNase I is included in the kit to remove contaminating genomic DNA during the workflow, ensuring the purity of the isolated mRNA and enhancing the reliability of downstream molecular applications.

Q: What are the storage requirements and shelf life for this kit?

A: Store the kit at 2-8C as instructed by the manufacturer. The typical shelf life is approximately 12 months from the date of manufacture, ensuring reagent integrity for repeated use.

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