Qiagen RT2 Profiler PCR Array

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Product Details:

Storage Instructions Store at -20C, avoid repeated freeze-thaw cycles
Shelf Life As per manufacturer, typically 12-24 months if stored properly
Function Simultaneous analysis of 84 pathway or disease-focused genes
Usage Type Laboratory
Measurement Range Wide dynamic range (36 log orders)
Instruments Type Molecular biology laboratory reagents
Accuracy Highly specific, designed for precise mRNA quantification
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Qiagen RT2 Profiler PCR Array Price And Quantity

Qiagen RT2 Profiler PCR Array Product Specifications

Instruments Type Molecular biology laboratory reagents
Operating Type Automatic
Accuracy Highly specific, designed for precise mRNA quantification
Technology Real-Time Quantitative PCR (qPCR) Array
Color Clear/transparent wells with white labeling
Measurement Range Wide dynamic range (36 log orders)
Weight Negligible
Dimension (L*W*H) Standard 96-well plate format
Portable Yes
Shelf Life As per manufacturer, typically 12-24 months if stored properly
Storage Instructions Store at -20C, avoid repeated freeze-thaw cycles
Material Highly purified plastic and functionalized plate well surfaces
Usage Type Laboratory
Condition New
Use Gene expression analysis, Pathway-specific PCR
Features Pre-validated gene-specific primer sets, easy workflow, high sensitivity, reproducibility, multi-pathway analysis
Power Source Not required (used with compatible PCR instruments)
Wall Mounted No
Equipment Type PCR Array
Real-Time Operation Yes
Function Simultaneous analysis of 84 pathway or disease-focused genes

Product Description

Brand Qiagen

Product Type RNA Kits

Pack Size 96 Wells

CAT No 330231

Application Molecular Reagent

RT2 Profiler PCR Arrays enable quick, reliable gene expression analysis of 170+ pathways, allowing researchers to spend less time at the bench and more time interpreting results. The arrays are pathway-focused panels of laboratory-verified qPCR assays, with integrated, patented controls to ensure a successful experiment every time. PhD-trained application specialists are available to provide technical support for the arrays, and each array can also be modified to suit unique experimental needs. For biomarker discoveries, we offer Pathway Plus PCR arrays and all arrays listed below for the Fluidigm BioMark Real-Time PCR System.

Comprehensive Gene Profiling

Analyze up to 84 target genes plus housekeeping gene, genomic DNA, reverse transcription, and PCR controls within a single run. This broad coverage facilitates focused and robust interrogation of signaling pathways or disease panels using standardized workflows and high-quality assays.

Optimized Format for Versatility

Available in both 96-well and 384-well plate formats, the RT2 Profiler PCR Array accommodates various throughput needs in the laboratory. Clear, labeled wells and easy set-up minimize error, supporting accurate results and streamlined operations across experimental scales.

Exceptional Sensitivity and Specificity

The array employs pre-validated, gene-specific primer sets on functionalized surfaces, ensuring highly sensitive and reproducible detection of mRNA. It provides a broad dynamic range (3-6 log orders) suitable for precise quantification of gene expression levels in a variety of research and diagnostic applications.

User-Friendly Workflow and Analysis

With straightforward workflows, minimal pipetting steps, and a dedicated online data analysis tool, researchers benefit from reduced hands-on time and intuitive result interpretation. Storage at -20C ensures long shelf life, and minimal sample input requirements streamline laboratory resource management.

FAQ's of Qiagen RT2 Profiler PCR Array:

Q: How do I use the RT2 Profiler PCR Array for gene expression analysis?

A: Simply prepare cDNA from your total RNA samples, add the designated amount (10-100 ng per reaction), and mix with the supplied PCR master mix. Dispense into the array plate wells following the recommended 25 l reaction volume (for 96-well format), then run on any compatible real-time PCR instrument. The workflow is straightforward, minimizing handling steps and reducing error.

Q: What kind of controls are included and what purpose do they serve?

A: Each array incorporates multiple controls: housekeeping gene controls for normalization, a genomic DNA control to detect DNA contamination, a reverse transcription control for RT efficiency, and a positive PCR control to validate the entire reaction process. These help ensure result accuracy and reliability throughout your experiments.

Q: When should I choose the 96-well format versus the 384-well format?

A: Select the 96-well format for smaller studies or pilot experiments and the 384-well format for larger-scale projects requiring higher throughput. Both formats offer similar performance and include the same control sets, so your choice depends on sample volume and laboratory needs.

Q: Where can I analyze my data after completing the PCR run?

A: Upon completing your real-time PCR, you can use Qiagen's online data analysis tool, which streamlines result interpretation and supports normalized, robust data output. This resource is accessible to all users and helps transform raw Ct values into meaningful biological insights.

Q: What sample types are compatible with the array?

A: The array is designed for cDNA generated from total RNA, making it suitable for a wide range of sample types and sources relevant to gene expression and pathway-directed studies in molecular biology.

Q: What are the storage requirements for the RT2 Profiler PCR Array, and how does this affect its shelf life?

A: Store the array plates at -20C and avoid repeated freeze-thaw cycles to maintain stability and performance. When appropriately stored, the product's shelf life is typically 12-24 months, as per manufacturer recommendations.

Q: What benefits does the RT2 Profiler PCR Array offer over traditional gene expression methods?

A: The array delivers high specificity, reproducibility, and sensitivity with a streamlined protocol, reducing time-to-result and risk of error. Its multiplex format enables simultaneous analysis of up to 84 genes plus controls, facilitating broad discovery and pathway profiling on one platform-ideal for biomarker research, disease studies, and drug screening.

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