Qiagen RT SYBR Green Fluor qPCR Mastermix (24)

Qiagen RT SYBR Green Fluor qPCR Mastermix (24)

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Product Details:

Usage Type Laboratory reagent (research use only)
Accuracy High amplification specificity and reproducibility
Measurement Range 10-10 copies (template dependent)
Shelf Life 12 months (when stored as recommended)
Function Enables real-time detection and quantification of PCR products
Storage Instructions Store at -20C; protect from light
Features High sensitivity, broad dynamic range, minimizes primer-dimer formation, compatible with cDNA and gDNA templates
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Qiagen RT SYBR Green Fluor qPCR Mastermix (24) Price And Quantity

Qiagen RT SYBR Green Fluor qPCR Mastermix (24) Product Specifications

Real-Time Operation Yes
Use qPCR amplification and real-time quantification of DNA targets
Storage Instructions Store at -20C; protect from light
Weight Approx. 10-20 g (24 reactions kit)
Dimension (L*W*H) Standard reagent vial size (pack of 24 reactions)
Instruments Type Real-Time PCR (qPCR) instruments
Features High sensitivity, broad dynamic range, minimizes primer-dimer formation, compatible with cDNA and gDNA templates
Accuracy High amplification specificity and reproducibility
Power Source Not required (used as reagent in instrument)
Wall Mounted No
Condition New
Operating Type Manual preparation, compatible with automated qPCR platforms
Material Proprietary enzyme blend, buffer solution, dNTPs, SYBR Green I dye
Shelf Life 12 months (when stored as recommended)
Color Clear to greenish (depending on SYBR Green dye concentration)
Function Enables real-time detection and quantification of PCR products
Usage Type Laboratory reagent (research use only)
Portable Yes (supplied in small vials)
Measurement Range 10-10 copies (template dependent)
Equipment Type qPCR Mastermix
Technology Real-Time SYBR Green Fluorescent Quantitative PCR

Product Description

Brand Qiagen

Product Type qPCR Kit

Pack Size 24 x 1.35 ml

CAT No 330513

Application Molecular Reagent

RT2SYBR Green qPCR Mastermixes are highly suited for real-time PCR applications using SYBR Green, and are required for guaranteed qPCR performance of the RT2qPCR Primer Assays, RT2Profiler PCR Arrays, RT2lncRNA qPCR Assays, and RT2lncRNA PCR Arrays. This Mastermix should be used in qPCR instruments under standard cycling conditions. RT2SYBR Green qPCR Mastermixes are also suitable for use with EpiTect ChIP and EpiTect Methyl II qPCR Assays and PCR Arrays.

Seamless qPCR Performance

Achieve accurate and highly sensitive DNA quantification using Qiagen RT SYBR Green Fluor qPCR Mastermix. Engineered for reliable results, this mix is equipped with a reference dye for normalization and is compatible with both cDNA and genomic DNA templates, making it an excellent choice for diverse research needs.

Designed for Ease of Use

The mastermix arrives as a 2x concentrated, ready-to-use format that simplifies your workflow. It integrates all necessary components, so manual preparation is minimizedsimply add your template and primers. The kit is compatible with manual and automated qPCR platforms for maximum flexibility in the laboratory.

Optimize Your Laboratory Workflow

With high amplification specificity, minimal primer-dimer formation, and broad dynamic range, this mastermix empowers researchers to confidently quantify low- or high-copy DNA targets. Each vial is designed for portability and convenience, ensuring consistent performance across various research settings.

FAQs of Qiagen RT SYBR Green Fluor qPCR Mastermix (24):

Q: How should the Qiagen RT SYBR Green Fluor qPCR Mastermix be stored to maintain its stability?

A: The mastermix should be stored at -20C and protected from light to ensure maximum shelf life and performance. Always avoid repeated freeze-thaw cycles to maintain reagent integrity.

Q: What types of templates can be used with this qPCR Mastermix?

A: This kit is compatible with both cDNA and genomic DNA templates, providing versatility for various real-time PCR applications.

Q: When is this mastermix recommended for use in the laboratory?

A: Use this mastermix for real-time quantitative PCR (qPCR) experiments when you require high sensitivity, broad dynamic range, and reliable quantification of DNA targets.

Q: Where can I use this mastermix within my workflow?

A: The mastermix can be incorporated into qPCR workflows on both manual and automated real-time PCR instruments. It is suitable for use in research laboratories focused on DNA quantification.

Q: What is the benefit of the included reference dye in the mastermix?

A: The reference dye enables normalization of results, correcting for pipetting or instrument variations and contributing to more accurate quantification during real-time PCR reactions.

Q: How does this mastermix contribute to the accuracy and reproducibility of qPCR results?

A: It utilizes HotStarTaq DNA Polymerase for high specificity, minimizes primer-dimer formation, and offers consistent results across a 1010 copy measurement range, promoting accurate and reproducible data.

Q: What process is involved in preparing the mastermix for a qPCR reaction?

A: Simply thaw the mastermix, vortex gently, and combine it with your DNA template and gene-specific primers. It arrives as a 2x concentrated, ready-to-use formulation for straightforward reaction setup.

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