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BioRad iScript Reverse Transcription Supermix, 100 x 20 l rxns, 400 l BioRad iScript Reverse Transcription Supermix, 100 x 20 l rxns, 400 l BioRad iScript Reverse Transcription Supermix, 100 x 20 l rxns, 400 l
BioRad iScript Reverse Transcription Supermix, 100 x 20 l rxns, 400 l
BioRad iScript Reverse Transcription Supermix, 100 x 20 l rxns, 400 l BioRad iScript Reverse Transcription Supermix, 100 x 20 l rxns, 400 l

BioRad iScript Reverse Transcription Supermix, 100 x 20 l rxns, 400 l

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Product Details:

  • Instruments Type Reagents for molecular biology
  • Usage Type Single use or multiple use under sterile conditions
  • Measurement Range Supports RNA input: 1 pg1 g per reaction
  • Accuracy High specificity and sensitivity for cDNA synthesis
  • Function Reverse transcription of RNA to cDNA
  • Shelf Life 1 year (when stored at -20C)
  • Features Ready-to-use master mix; optimized for high efficiency; minimizes pipetting steps; RNase inhibitor included
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BioRad iScript Reverse Transcription Supermix, 100 x 20 l rxns, 400 l Price And Quantity

BioRad iScript Reverse Transcription Supermix, 100 x 20 l rxns, 400 l Product Specifications

  • Not Applicable (Reagent vial)
  • 1 year (when stored at -20C)
  • Single use or multiple use under sterile conditions
  • Synthesis of complementary DNA (cDNA) from RNA templates
  • cDNA synthesis via reverse transcription
  • New
  • Supports RNA input: 1 pg1 g per reaction
  • Manual operation for in vitro laboratory use
  • Reverse Transcription Supermix Kit
  • Yes (reagent format)
  • Store at -20C; avoid repeated freeze-thaw cycles
  • Ready-to-use master mix; optimized for high efficiency; minimizes pipetting steps; RNase inhibitor included
  • 400 l total reagent volume
  • Reagents for molecular biology
  • Reverse transcription of RNA to cDNA
  • Liquid enzyme formulation
  • High specificity and sensitivity for cDNA synthesis
  • Compatible with real-time PCR
  • No
  • Clear or pale yellow liquid
  • Provided on product label
  • 1708840
  • Any thermal cycler for reverse transcription
  • Supermix; 100 x 20 l reactions
  • Usually 2060 minutes depending on protocol
  • Optimized buffer for reverse transcription
  • -20C
  • Non-hazardous under normal laboratory conditions
  • 400 l total
  • Reverse transcriptase and RNase inhibitor
  • qPCR, endpoint RT-PCR, gene expression analysis

Product Description

Brand                          BioRad

Product Type              RT-PCR Kit

Pack Size                    4 x 100 ul

CAT No                       1708841

Application                 Molecular Biology

The iScript reverse transcription supermix for RT-qPCR is a simple, fast, and sensitive first-strand cDNA synthesis kit for gene expression analysis using real-time qPCR. In one tube, the preblended 5x supermix contains all the necessary components except RNA template for reverse transcription.




Efficient cDNA Synthesis for All Applications

BioRad iScript Reverse Transcription Supermix is optimized for rapid and accurate reverse transcription. Suitable for qPCR, endpoint RT-PCR, and gene expression studies, it enables researchers to achieve high yields of cDNA from as little as 1 pg to 1 g of RNA per reaction, ensuring sensitivity and broad dynamic range. The inclusion of an RNase inhibitor preserves RNA integrity for dependable results.


User-Friendly and Versatile Format

This Supermix arrives as a liquid, ready-to-use master mix, minimizing pipetting steps and potential errors. The 400 l pack facilitates up to 100 reactions, supporting both single and multiple uses within sterile environments. Its compatibility with any standard thermal cycler allows for smooth integration into existing laboratory protocols.


Storage, Stability, and Safety

The kit should be stored at -20C to retain optimal enzymatic activity and a shelf life of up to one year. It is formulated as non-hazardous for laboratory handling, and users are advised to avoid repeated freeze-thaw cycles. Each vial's lot number ensures traceability for quality assurance and regulatory compliance.

FAQ's of BioRad iScript Reverse Transcription Supermix, 100 x 20 Al rxns, 400 Al:


Q: How should I store the BioRad iScript Reverse Transcription Supermix for maximum stability?

A: Store the Supermix at -20C immediately upon arrival. To preserve the reagent's efficacy, minimize repeated freeze-thaw cycles. The product remains stable for up to one year when properly stored.

Q: What makes this Supermix suitable for gene expression analysis and qPCR applications?

A: The Supermix includes a highly efficient reverse transcriptase, an RNase inhibitor, and an optimized buffer, providing high specificity and sensitivity. This leads to robust and reproducible cDNA synthesis for precise qPCR and gene expression analysis.

Q: When is it appropriate to use this Supermix during molecular biology workflows?

A: Use the Supermix whenever you need to synthesize complementary DNA (cDNA) from RNA templates, such as before conducting qPCR, endpoint RT-PCR, or gene expression profiling. It is compatible with any reverse transcription thermal cycler protocol.

Q: Where can this Supermix be used and with which equipment is it compatible?

A: The Supermix is suitable for use in standard molecular biology laboratories and is compatible with any thermal cycler designed for reverse transcription reactions, providing flexibility and broad applicability.

Q: What is the process for performing a reverse transcription reaction with this kit?

A: Simply combine your RNA template with the iScript Supermix according to the included protocol, then run the reaction on your thermal cycler for 20-60 minutes, depending on specific assay requirements. The reagent minimizes pipetting, reducing sample handling times.

Q: What are the key benefits of using BioRad iScript Supermix over conventional reverse transcription reagents?

A: This Supermix streamlines workflow with a ready-to-use format, reduces pipetting steps, and includes RNase inhibitor for RNA protection. These features result in rapid, reliable, and reproducible cDNA synthesis with high efficiency.

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