Qiagen QIAamp 96 DNA Blood Kit (12)

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Product Details:

Usage Type Laboratory
Features High-throughput, reproducible DNA isolation, suitable for automated platforms
Function DNA purification from whole blood in a 96-well format
Measurement Range Typical DNA yield: up to 10 g/sample (dependent on input volume)
Shelf Life 12 months from manufacturing date
Storage Instructions Store at room temperature (1525 C)
Accuracy High yield and purity suitable for downstream applications
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Qiagen QIAamp 96 DNA Blood Kit (12) Price And Quantity

Qiagen QIAamp 96 DNA Blood Kit (12) Product Specifications

Wall Mounted No
Function DNA purification from whole blood in a 96-well format
Features High-throughput, reproducible DNA isolation, suitable for automated platforms
Real-Time Operation No
Condition New
Equipment Type DNA extraction kit
Portable Yes
Dimension (L*W*H) Kit Box: Approx. 32 x 22 x 18 cm
Operating Type Manual
Weight Approx. 3.2 kg per kit
Technology Silica membrane-based 96-well plate technology
Instruments Type Nucleic acid extraction kit
Usage Type Laboratory
Accuracy High yield and purity suitable for downstream applications
Color White/clear (kit components)
Storage Instructions Store at room temperature (1525 C)
Use For high-throughput purification of genomic DNA from blood samples
Material High-quality plastic (plates, tubes), proprietary buffers and reagents
Shelf Life 12 months from manufacturing date
Measurement Range Typical DNA yield: up to 10 g/sample (dependent on input volume)

Binding Capacity Up to 10 g DNA per well
Pack Size 12 x 96 well plates (sufficient for 1152 preps)
Hazardous Material Some buffers may contain guanidine salts (refer to MSDS)
Elution Volume 100200 l per sample
Catalog Number 51161
Application PCR, real-time PCR, genotyping, sequencing, and other molecular assays
Purity (A260/A280) 1.71.9 (typical)
Automation Compatibility Compatible with QIAGEN BioRobot systems and multichannel pipettes
Sample Input Volume Up to 200 l whole blood per well
Kit Format 96-well plate (12 plates per kit)

Product Description

Brand Qiagen

Product Type PCR Kits

Pack Size 12 x 96 Wells

CAT No 51162

Application Molecular Reagent

QIAamp DNA Blood Kits provide silica-membrane-based DNA purification from whole blood, plasma, serum and other body fluids. The kits are designed for a range of sample sizes from 200 l up to 10 ml fresh or frozen human whole blood. QIAamp spin columns can be easily processed in a centrifuge or on vacuum manifolds. A convenient 96-well format using centrifugation enables purification of DNA for labs that need high-throughput DNA purification from blood, buffy coat, plasma, serum, bone marrow, lymphocytes and body fluids. A dedicated kit is also available for automated purification of 1 12 samples on the QIAcube Connect.

High-Throughput DNA Isolation for Modern Labs

The QIAamp 96 DNA Blood Kit is engineered for laboratories requiring fast, reproducible extraction of genomic DNA from whole blood. The 96-well plate format allows simultaneous processing of multiple samples, significantly increasing productivity. This kit is suitable for use with automated platforms or manual multichannel pipettes, making it versatile for different lab setups and workloads.

Consistent Yield and Purity for Downstream Applications

Designed to deliver DNA with typical A260/A280 ratios of 1.7-1.9, the QIAamp 96 DNA Blood Kit ensures high quality extractions for applications including PCR, real-time PCR, sequencing, and genotyping. Each prep can yield up to 10 g DNA, giving you reliable output for demanding molecular assays and reducing variability in results.

Easy Storage, Handling, and Safety

The kit is supplied in a compact box (approx. 32 x 22 x 18 cm, 3.2 kg) and requires storage at room temperature (15-25C), saving valuable fridge or freezer space. Some buffers contain guanidine salts; consult the MSDS for safe handling guidance. With a shelf life of 12 months, each kit is ready to support sustained projects over time.

FAQ's of Qiagen QIAamp 96 DNA Blood Kit (12):

Q: How do I use the QIAamp 96 DNA Blood Kit for DNA extraction from blood samples?

A: To use the kit, simply load up to 200 l whole blood per well in the provided 96-well plates, add the proprietary buffers, and follow the silica membrane-based purification protocol. Processing can be performed manually with a multichannel pipette or automated using a QIAGEN BioRobot system. DNA is eluted in 100-200 l per well, suitable for downstream applications.

Q: What is the typical DNA yield and purity achieved with this kit?

A: Each well can yield up to 10 g of DNA, depending on the input volume. The purified DNA typically achieves an A260/A280 purity ratio of 1.7-1.9, which meets quality standards for PCR, sequencing, and genotyping.

Q: When should I consider using this kit over other extraction formats?

A: The QIAamp 96 DNA Blood Kit is ideal for laboratories that require high-throughput processing, enabling extraction from 96 samples in parallel. It is especially recommended for large projects, clinical studies, or any scenario where consistent, reproducible results are critical.

Q: Where can this DNA extraction kit be used?

A: This kit is intended for laboratory use, including research, clinical, or core facilities that process whole blood samples for molecular biology applications. It is suitable for labs in dealer, distributor, exporter, manufacturer, supplier, trader, and wholesaler networks, particularly in India.

Q: What is the benefit of the 96-well plate format and automation compatibility?

A: The 96-well plate design allows simultaneous purification of multiple samples, saving time and reducing manual effort. Automation compatibility with QIAGEN BioRobot systems increases efficiency and standardization, making it perfect for high-throughput workflows.

Q: How should I store and handle the kit for optimal performance?

A: Store the kit at room temperature (15-25C) and refer to the product's MSDS for safety, as some buffers contain guanidine salts. Proper storage and handling preserve the kit's performance and ensure a shelf life of up to 12 months from the manufacturing date.

Q: What downstream applications is the purified DNA suitable for?

A: The DNA isolated using this kit is suitable for a wide range of applications, including PCR, real-time PCR, genotyping, sequencing, and other molecular assays that require high-quality genomic DNA.

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