Product Description
Brand Qiagen
Product Type PCR Kits
Pack Size 50T
CAT No 77164
Application Molecular Reagent
exoRNeasy Kits use membrane affinity spin columns to efficiently capture exosomes and other EVs from most biofluids, including urine, serum, plasma, CSF and cell culture supernatant. The kits then use proven RNeasy MinElute spin columns to isolate RNA from the EVs. The midi column format enables efficient processing of smaller sample volumes (up to 1 ml serum/plasma or 4 ml of urine), while the maxi column format allows the use of large sample volumes, up to 4 ml serum/plasma, 16 ml urine or 32 ml cell culture supernatant, to detect low-abundance RNAs with high confidence. The results are fast, consistent and highly suited to sensitive downstream applications. The RNA isolation part of the protocol using exoRNeasy Kits can be automated on theQIAcube Connect.
Reliable Exosomal RNA ExtractionQiagen's exoRNeasy Maxi Kit offers highly efficient extraction and purification of exosomal RNA from a broad range of cell-free biofluids. Its advanced membrane affinity spin column ensures selective capture, delivering high-quality RNA suitable for sensitive downstream analysis methods including RT-PCR, NGS, and microarray. This reliability streamlines lab workflows and supports comprehensive RNA research.
Flexible and Scalable WorkflowPerfect for medium to high throughput laboratories, the exoRNeasy Maxi Kit is scalable for larger sample volumes-from 1 ml to 4 ml per prep. Whether processing a few or many samples, it ensures consistent results, enabling labs to adapt to changing project needs while maintaining accuracy and reproducibility in exosomal RNA yield.
Optimized for ConsistencyWith a preferred elution volume of 14-30 l per prep and the capacity to bind up to 500 l of biofluid, the kit delivers robust and repeatable performance. This optimization supports confident data generation in downstream RNA analysis, providing researchers with reliable results essential for studies in disease biomarkers and RNA biology.
FAQ's of Qiagen exoRNeasy Maxi Kit (50):
Q: How does the Qiagen exoRNeasy Maxi Kit work for exosomal RNA extraction?
A: The kit utilizes a manual, membrane affinity spin column technology to selectively capture exosomal RNA from cell-free biofluids. After binding the target RNA to the column, impurities are washed away, and the purified RNA is then eluted for downstream applications.
Q: What types of samples can be processed with this kit?
A: The exoRNeasy Maxi Kit is compatible with plasma, serum, and various other cell-free biofluids, making it highly versatile for RNA analysis from different biological sources.
Q: When should I use the exoRNeasy Maxi Kit in my workflow?
A: Use this kit when you require efficient isolation and purification of exosomal RNA for applications like miRNA, lncRNA, or total exosomal RNA profiling in downstream analyses such as RT-PCR, NGS, or microarray.
Q: Where can the kit be stored and what are the storage instructions?
A: Most reagents in the kit can be stored at room temperature, but certain components must be kept at 2-8 C. Always refer to the specific storage guidelines provided in the package to ensure optimal reagent performance.
Q: What is the typical process for using the exoRNeasy Maxi Kit?
A: First, mix your biofluid sample with the provided binding reagents, apply it to the spin column, and perform the recommended washing steps. Finally, elute highly purified exosomal RNA in 14-30 l for direct use in molecular assays.
Q: How much sample volume and biofluid does the kit support per preparation?
A: Each preparation can accommodate 1.0 ml to 4.0 ml of starting biofluid, binding up to 500 l per prep. This makes it suitable for processing both small and large sample volumes in a scalable manner.
Q: What are the primary benefits of using the exoRNeasy Maxi Kit for RNA studies?
A: Key advantages include high and consistent RNA recovery, compatibility with multiple downstream technologies, efficiency with large-volume samples, portability, silent operation, and a reputation for reliable results in laboratory research.