Product Description
Brand Qiagen
Product Type PCR Kit
Pack Size 24 x 96 Wells
CAT No 28183
Application Molecular Reagent
QIAquick 96 PCR Purification Kits provide 96-well plates, buffers, and collection tubes for high-throughput silica-membrane-based purification of PCR products >100 bp in size. DNA up to 10 kb is purified using a simple and fast bindwashelute procedure and an elution volume of 6080 l (resulting in an eluate volume of 4060 l). The cleanup procedure can be fully automated on the BioRobot Universal workstation using the QIAquick 96 PCR BioRobot Kit.
High-Throughput DNA Purification Made EasyThe QIAquick 96 PCR Purification Kit streamlines DNA clean-up from PCR and enzymatic reactions using a convenient 96-well plate format. Laboratories can process up to 24 plates (2304 samples) per kit, dramatically increasing productivity. The color-coded buffers and transparent plates help ensure straightforward, error-free workflow integration for both manual and automated processing systems.
Optimal Recovery and Reliable ResultsDesigned with a proprietary silica membrane, the QIAquick kit provides efficient DNA recovery rates of 70% or higher for fragments ranging from 70 bp to 10 kb. This high performance ensures that your purified DNA is of suitable quality for downstream applications such as sequencing, cloning, or molecular analysis.
Flexible and User-Friendly Laboratory SolutionCompatible with automated instruments as well as manual protocols, this kit supports adaptability in varying lab setups. With a shelf life of 12 months and simple storage at room temperature, it delivers reliability, ease of use, and convenience for busy research environments.
FAQs of Qiagen QIAquick 96 PCR Purification Kit (24):
Q: How does the QIAquick 96 PCR Purification Kit work?
A: The kit uses a silica membrane-based technology within a 96-well plate format. DNA binds to the membrane in the presence of a binding buffer, while impurities such as enzymes, salts, primers, and dNTPs are removed through washing. The purified DNA is then eluted using an appropriate buffer, yielding high-quality DNA fragments ready for downstream applications.
Q: What types of samples can be purified using this kit?
A: The QIAquick 96 PCR Purification Kit is intended for PCR products, enzymatic reactions, and various DNA fragments ranging from 70 bp to 10 kb in length. It is effective for most molecular biology applications requiring clean DNA.
Q: When is it beneficial to use this high-throughput kit?
A: This kit is particularly advantageous when processing large numbers of PCR or enzymatic reaction samples, such as in core facilities, high-throughput screening projects, or research requiring rapid and parallel DNA purification from many samples.
Q: Where should the kit and its components be stored?
A: Store the kit and all reagents at room temperature (1525C), ensuring that buffers are kept tightly closed. This maintains product quality over its 12-month shelf life from the date of manufacture.
Q: Can this kit be used with automated systems, or is it strictly manual?
A: The QIAquick 96 kit is compatible with both manual and automated processing platforms, allowing integration into robotic workflows for higher efficiency in sample handling, as well as flexibility for manual purification when needed.
Q: What is the maximum DNA binding capacity per well?
A: Each well can bind up to 5 g of DNA, efficiently handling sample volumes up to 100 l. This enables reliable recovery even from samples with higher DNA concentrations.
Q: What are the practical benefits of using this kit in the laboratory?
A: The kit offers high-throughput capability, efficient removal of contaminants, excellent DNA recovery rates (70%), and compatibility with various workflows. It also supports rapid processing and consistency, saving time and reducing operator error in busy molecular biology labs.