Qiagen QIAamp DNA Blood Midi Kit (100)

Get a Price/Quote

Product Details:

Function DNA extraction and purification
Shelf Life Generally 1224 months (refer to kit manual)
Usage Type Laboratory research or clinical
Instruments Type Sample processing kit
Accuracy High purification efficiency (>98% typical recovery)
Storage Instructions Store at room temperature (1525C), reagents may require refrigeration after opening
Measurement Range Suitable for 0.52 ml blood per prep, up to 500 g DNA per kit
Click to View more
Share Your Product:

Request to Call Back Send Inquiry

Qiagen QIAamp DNA Blood Midi Kit (100) Price And Quantity

Qiagen QIAamp DNA Blood Midi Kit (100) Product Specifications

Features High DNA yield, easy-to-use protocol, consistent results
Operating Type Manual
Storage Instructions Store at room temperature (1525C), reagents may require refrigeration after opening
Material Plastic (columns, tubes), reagents in solution
Real-Time Operation No
Measurement Range Suitable for 0.52 ml blood per prep, up to 500 g DNA per kit
Portable Yes
Power Source Not required (manual kit)
Equipment Type DNA Extraction Kit
Wall Mounted No
Instruments Type Sample processing kit
Accuracy High purification efficiency (>98% typical recovery)
Use Purification and isolation of genomic DNA from blood samples
Color White/transparent (columns, collection tubes)
Shelf Life Generally 1224 months (refer to kit manual)
Condition New
Usage Type Laboratory research or clinical
Function DNA extraction and purification
Technology Silica membrane technology

Sample Throughput Low to medium
Cat. No. 51185
Binding Capacity 20 g DNA per column
Hazardous Material Contains guanidine salts (handle with care)
Elution Volume 100300 l per prep
Time per prep Approximately 4060 minutes
Compatibility Compatible with both fresh and frozen blood samples
Sample Type Whole blood, buffy coat, body fluids
Kit Size 100 preps
Intended Use Molecular biology applications (e.g., PCR, genotyping, sequencing)

Product Description

Brand Qiagen

Product Type QIAamp

Pack Size 100T

CAT No 51185

Application Molecular Reagent

QIAamp DNA Blood Kits provide silica-membrane-based DNA purification from whole blood, plasma, serum and other body fluids. The kits are designed for a range of sample sizes from 200 l up to 10 ml fresh or frozen human whole blood. QIAamp spin columns can be easily processed in a centrifuge or on vacuum manifolds. A convenient 96-well format using centrifugation enables purification of DNA for labs that need high-throughput DNA purification from blood, buffy coat, plasma, serum, bone marrow, lymphocytes and body fluids. A dedicated kit is also available for automated purification of 112 samples on the QIAcube Connect.

Efficient Genomic DNA Extraction

The QIAamp DNA Blood Midi Kit enables fast and effective purification of genomic DNA from a variety of blood-derived samples. Its advanced silica membrane technology minimizes sample processing time while maximizing yield and purity, making it ideal for molecular biology needs in laboratory settings.

High Purity and Consistent Results

Achieve over 98% recovery rates of DNA with this kit, ensuring reliable results for sensitive applications such as PCR, genotyping, or sequencing. Each kit is rigorously tested for consistency, guaranteeing performance across all 100 preps.

User-Friendly and Versatile

This DNA extraction kit is simple to use, requiring no specialized instruments or power source-making it highly portable. The kit is suitable for processing various sample types, including both fresh and frozen blood, adding to its versatility in research and clinical labs.

FAQ's of Qiagen QIAamp DNA Blood Midi Kit (100):

Q: How does the QIAamp DNA Blood Midi Kit work for DNA extraction?

A: The kit uses a spin-column protocol with advanced silica membrane technology to bind DNA selectively. After lysing the sample and adding the binding solution, DNA is captured on the membrane, washed to remove impurities, and then eluted in the desired volume for downstream molecular biology applications.

Q: What types of samples are compatible with this kit?

A: The kit is designed for the purification of genomic DNA from whole blood, buffy coat, and various body fluids. It accommodates both fresh and frozen samples, offering flexibility depending on your sample storage conditions.

Q: When should I use the QIAamp DNA Blood Midi Kit for my laboratory work?

A: This kit is recommended whenever you require high-purity, high-yield DNA for molecular biology experiments such as PCR, sequencing, or genotyping, especially when working with sample volumes between 0.5 and 2 ml per prep.

Q: Where should the reagents and components be stored?

A: Store the kit at room temperature (15-25C) as recommended. However, note that certain reagents may need refrigeration after opening; always refer to the kit manual for specific storage instructions.

Q: What is the general process and time required per DNA purification?

A: Each DNA extraction takes approximately 40-60 minutes from sample lysis to elution. The process is carried out manually using provided plastic columns and collection tubes, requiring no specialized equipment.

Q: Is this kit portable and suitable for on-site sample processing?

A: Yes, the kit is fully manual and does not require external power or specialized instruments, making it portable and suitable for laboratories that require flexibility in sample processing locations.

Q: What benefits does the QIAamp DNA Blood Midi Kit offer for laboratory research?

A: The key benefits include high DNA yield, superior purity, consistent results, ease of use, and compatibility with various blood sample types. Its reliability enhances the success rate of downstream applications such as PCR and sequencing.

Tell us about your requirement

Price:

Quantity

Select Unit

50
100
200
250
500
1000+

Additional detail

Mobile number

Other Products in 'Medical Reagents' category

FUJIFILM FUJI DRI-CHEM SLIDE TG-P III Wall Mounted : No Use : Quantitative Determination of Triglycerides (TG) in Blood/Serum/Plasma Condition : New Usage Type : Single Use/Test Equipment Type : Dry Chemistry Analyzer Reagent Slide Instruments Type : Compatible with FUJIFILM DRICHEM Analyzers Technology : DRICHEM (Dry Biochemical) Technology Operating Type : Automatic Real-Time Operation : Yes Material : Other, Multilayer chemical reagent film Portable : Yes (used with portable analyzers) Accuracy : High, as per FUJIFILM standards Weight : ~2 grams per slide Color : White/Blue (slide coloration) Shelf Life : 24 months (unopened) Function : Triglycerides measurement slide Features : No liquid reagent preparation, Easy handling, Stable storage Storage Instructions : 28C, dry and away from direct sunlight Noise Level : Silent Operation Dimension (L*W*H) : Slide Size: Approx. 46 mm x 24 mm x 1 mm Measurement Range : Primarily 101,200 mg/dL (may vary by analyzer)

Send Inquiry

BioRad ReadyPrep Protein Extraction Kit (Total Protein) Wall Mounted : No Use : Preparation of total protein extracts from biological samples Condition : New Usage Type : Laboratory / Research Equipment Type : Protein Extraction Kit Instruments Type : Protein biochemistry laboratory kit Technology : Protein extraction and solubilization Operating Type : Manual Real-Time Operation : No Material : Other, Biochemical reagents, plastic components Portable : Yes Accuracy : Consistent protein yields Weight : Approx. 0.5 kg Color : White/Blue (kit and reagents) Shelf Life : Refer to label (typically 1224 months from manufacture) Function : Lysis and extraction of total proteins Features : Readytouse reagents, suitable for a wide range of tissues and cells, rapid procedure Storage Instructions : Store at 28C Dimension (L*W*H) : Kit box: standard laboratory kit size Measurement Range : Suitable for various sample masses (check kit instructions)

Send Inquiry

FUJIFILM FUJI DRI-CHEM SLIDE TP-P III Wall Mounted : No Use : Clinical laboratory test for total protein measurement in serum/plasma Condition : New Usage Type : Singleuse, disposable Equipment Type : Dry Chemistry Analyzer Slide Instruments Type : Reagent Slide for Chemistry Analyzer Technology : Dry Chemistry (DRICHEM) Operating Type : Automatic Real-Time Operation : Yes Material : Other, Multilayered reagent film Portable : Yes Accuracy : High, Clinical Grade Weight : Approx. few grams per slide Color : White Shelf Life : 18 Months Function : Measurement of total protein in blood samples Features : Fast results, easy to use, high accuracy, no liquid reagents needed Storage Instructions : Store at 2 8C, avoid direct sunlight Noise Level : Low Dimension (L*W*H) : Cartridge Slide Type Measurement Range : TP measurement: 3.0 12.0 g/dL

Send Inquiry

Erba Mannheim ERBA H360 Lyse Wall Mounted : No Use : Blood Cell Lysis for Hematology Analysis Condition : New Usage Type : Laboratory Reagent Equipment Type : Hematology Reagent Instruments Type : Clinical Laboratory Reagents Technology : Automated Hematology Analyzer Compatible Operating Type : Other, Fully Automatic Real-Time Operation : Yes Material : Other, Liquid Chemical Solution Portable : Yes (Canister Design) Accuracy : Optimized for Consistent Cell Lysis Weight : 5 Litres (Net Content); Packaging may vary Color : Colorless to Pale Yellow Shelf Life : 18 Months Function : Lyses Red Blood Cells for WBC Differentiation Features : Precise Cell Lysis, Ready to Use, Compatible with ERBA H360 Storage Instructions : Store at Room Temperature (1530 C), Away from Direct Sunlight

Send Inquiry

SEND INQUIRY CALL ME FREE