Qiagen QIAamp DNA Blood Midi Kit (100)

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Product Details:

Measurement Range Suitable for 0.52 ml blood per prep, up to 500 g DNA per kit
Accuracy High purification efficiency (>98% typical recovery)
Function DNA extraction and purification
Storage Instructions Store at room temperature (1525C), reagents may require refrigeration after opening
Features High DNA yield, easy-to-use protocol, consistent results
Usage Type Laboratory research or clinical
Shelf Life Generally 1224 months (refer to kit manual)
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Qiagen QIAamp DNA Blood Midi Kit (100) Price And Quantity

Qiagen QIAamp DNA Blood Midi Kit (100) Product Specifications

Wall Mounted No
Measurement Range Suitable for 0.52 ml blood per prep, up to 500 g DNA per kit
Function DNA extraction and purification
Real-Time Operation No
Accuracy High purification efficiency (>98% typical recovery)
Condition New
Equipment Type DNA Extraction Kit
Features High DNA yield, easy-to-use protocol, consistent results
Storage Instructions Store at room temperature (1525C), reagents may require refrigeration after opening
Portable Yes
Power Source Not required (manual kit)
Shelf Life Generally 1224 months (refer to kit manual)
Material Plastic (columns, tubes), reagents in solution
Instruments Type Sample processing kit
Operating Type Manual
Use Purification and isolation of genomic DNA from blood samples
Color White/transparent (columns, collection tubes)
Usage Type Laboratory research or clinical
Technology Silica membrane technology

Binding Capacity 20 g DNA per column
Kit Size 100 preps
Sample Throughput Low to medium
Sample Type Whole blood, buffy coat, body fluids
Hazardous Material Contains guanidine salts (handle with care)
Elution Volume 100300 l per prep
Time per prep Approximately 4060 minutes
Compatibility Compatible with both fresh and frozen blood samples
Cat. No. 51185
Intended Use Molecular biology applications (e.g., PCR, genotyping, sequencing)

Product Description

Brand Qiagen

Product Type QIAamp

Pack Size 100T

CAT No 51185

Application Molecular Reagent

QIAamp DNA Blood Kits provide silica-membrane-based DNA purification from whole blood, plasma, serum and other body fluids. The kits are designed for a range of sample sizes from 200 l up to 10 ml fresh or frozen human whole blood. QIAamp spin columns can be easily processed in a centrifuge or on vacuum manifolds. A convenient 96-well format using centrifugation enables purification of DNA for labs that need high-throughput DNA purification from blood, buffy coat, plasma, serum, bone marrow, lymphocytes and body fluids. A dedicated kit is also available for automated purification of 112 samples on the QIAcube Connect.

Efficient Genomic DNA Extraction

The QIAamp DNA Blood Midi Kit enables fast and effective purification of genomic DNA from a variety of blood-derived samples. Its advanced silica membrane technology minimizes sample processing time while maximizing yield and purity, making it ideal for molecular biology needs in laboratory settings.

High Purity and Consistent Results

Achieve over 98% recovery rates of DNA with this kit, ensuring reliable results for sensitive applications such as PCR, genotyping, or sequencing. Each kit is rigorously tested for consistency, guaranteeing performance across all 100 preps.

User-Friendly and Versatile

This DNA extraction kit is simple to use, requiring no specialized instruments or power source-making it highly portable. The kit is suitable for processing various sample types, including both fresh and frozen blood, adding to its versatility in research and clinical labs.

FAQ's of Qiagen QIAamp DNA Blood Midi Kit (100):

Q: How does the QIAamp DNA Blood Midi Kit work for DNA extraction?

A: The kit uses a spin-column protocol with advanced silica membrane technology to bind DNA selectively. After lysing the sample and adding the binding solution, DNA is captured on the membrane, washed to remove impurities, and then eluted in the desired volume for downstream molecular biology applications.

Q: What types of samples are compatible with this kit?

A: The kit is designed for the purification of genomic DNA from whole blood, buffy coat, and various body fluids. It accommodates both fresh and frozen samples, offering flexibility depending on your sample storage conditions.

Q: When should I use the QIAamp DNA Blood Midi Kit for my laboratory work?

A: This kit is recommended whenever you require high-purity, high-yield DNA for molecular biology experiments such as PCR, sequencing, or genotyping, especially when working with sample volumes between 0.5 and 2 ml per prep.

Q: Where should the reagents and components be stored?

A: Store the kit at room temperature (15-25C) as recommended. However, note that certain reagents may need refrigeration after opening; always refer to the kit manual for specific storage instructions.

Q: What is the general process and time required per DNA purification?

A: Each DNA extraction takes approximately 40-60 minutes from sample lysis to elution. The process is carried out manually using provided plastic columns and collection tubes, requiring no specialized equipment.

Q: Is this kit portable and suitable for on-site sample processing?

A: Yes, the kit is fully manual and does not require external power or specialized instruments, making it portable and suitable for laboratories that require flexibility in sample processing locations.

Q: What benefits does the QIAamp DNA Blood Midi Kit offer for laboratory research?

A: The key benefits include high DNA yield, superior purity, consistent results, ease of use, and compatibility with various blood sample types. Its reliability enhances the success rate of downstream applications such as PCR and sequencing.

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