Thermo Fisher Scientific Applied Biosystems TaqMan PreAmp Master Mix (cDNA pre-amplification for qPCR)

Thermo Fisher Scientific Applied Biosystems TaqMan PreAmp Master Mix (cDNA pre-amplification for qPCR)

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Product Details:

Function Pre-amplifies low copy number cDNA targets for sensitive qPCR
Storage Instructions Store at 20C
Shelf Life See product label (typically 12-18 months from manufacturing date)
Features Streamlines multiplex pre-amplification; compatible with nanogram to picogram RNA input
Accuracy High fidelity during pre-amplification
Measurement Range Suitable for low abundant target transcripts
Usage Type Laboratory, research
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Thermo Fisher Scientific Applied Biosystems TaqMan PreAmp Master Mix (cDNA pre-amplification for qPCR) Price And Quantity

Thermo Fisher Scientific Applied Biosystems TaqMan PreAmp Master Mix (cDNA pre-amplification for qPCR) Product Specifications

Wall Mounted No
Operating Type Pre-amplification reaction mix
Technology qPCR Pre-amplification (TaqMan chemistry)
Function Pre-amplifies low copy number cDNA targets for sensitive qPCR
Use Pre-amplification of cDNA target sequences prior to qPCR
Measurement Range Suitable for low abundant target transcripts
Accuracy High fidelity during pre-amplification
Material Proprietary reagent mixture (contains enzymes and buffers)
Usage Type Laboratory, research
Color Clear to slightly yellow solution
Condition New
Real-Time Operation Yes, designed for real-time qPCR workflows
Instruments Type Compatible with Applied Biosystems real-time PCR systems
Dimension (L*W*H) Not Applicable (supplied in reagent vials)
Features Streamlines multiplex pre-amplification; compatible with nanogram to picogram RNA input
Weight Not Applicable (supplied in kit/vial format)
Power Source Not Applicable (used in PCR instruments)
Equipment Type Master Mix for cDNA Pre-amplification
Shelf Life See product label (typically 12-18 months from manufacturing date)
Portable Yes, as a laboratory reagent
Storage Instructions Store at 20C

Product Description

Brand	Thermo Fisher Scientific
Product Type	PreAmp Master Mix
200 Reactions	200 Reactions
CAT No	4488593
Application	Molecular Biology

TaqMan PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions.

Reliable Pre-Amplification for Low Input and Rare Transcript Detection

The TaqMan PreAmp Master Mix is engineered to boost sensitivity in gene expression studies, especially when starting with nanogram to picogram levels of RNA. By enabling pre-amplification of low copy number targets, it provides researchers with the power to detect rare transcripts with confidence before performing qPCR. Its compatibility with TaqMan Gene Expression Assays broadens research possibilities in biomarker discovery and other quantitative gene analysis applications.

Convenient Format for Real-Time qPCR Workflows

Supplied in clear to slightly yellow 2 x 1.5 mL vials, this reagent offers flexibility for multiple laboratory setups. Its 2X master mix formulation simplifies setup and reduces handling errors, ensuring consistent performance across experiments. The hot-start DNA polymerase and high-purity molecular biology grade reagents guarantee specificity, making it well-suited for real-time qPCR amplification across Applied Biosystems PCR platforms.

FAQ's of Thermo Fisher Scientific Applied Biosystems TaqMan PreAmp Master Mix (cDNA pre-amplification for qPCR):

Q: How is the TaqMan PreAmp Master Mix used in low input RNA workflows?

A: The TaqMan PreAmp Master Mix is specifically designed to pre-amplify cDNA generated from nanogram to picogram quantities of RNA, making it highly suitable for experiments with limited sample material. By enriching low copy number target sequences, researchers can achieve sufficient material for downstream qPCR analysis.

Q: What types of assays is this master mix compatible with?

A: The PreAmp Master Mix is fully compatible with Applied Biosystems TaqMan Gene Expression Assays and custom TaqMan assays, allowing flexible gene expression profiling and enabling multiplexing for biomarker discovery.

Q: When should I use pre-amplification with this master mix in my workflow?

A: Pre-amplification is recommended when working with scarce or precious RNA samples where target abundance may be too low for direct quantification by qPCR. Use the TaqMan PreAmp Master Mix after reverse transcription to amplify target cDNAs prior to qPCR detection.

Q: Where can I purchase additional sizes or obtain this product in India?

A: The TaqMan PreAmp Master Mix can be sourced in India through official dealers, distributors, exporters, manufacturers, suppliers, traders, and wholesalers of Thermo Fisher Scientific and Applied Biosystems products. Various package sizes are available to suit different laboratory needs.

Q: What is the process for a typical pre-amplification reaction using this mix?

A: A standard reaction involves mixing cDNA with the 2X PreAmp Master Mix and target-specific TaqMan assay mix in a 20 L reaction volume, as recommended by the manufacturer. Following a defined thermal cycling protocol, pre-amplified products are then ready for quantitative analysis by qPCR.

Q: How does this product ensure high specificity and sensitivity during pre-amplification?

A: The master mix is formulated with hot-start DNA polymerase, dNTPs, and proprietary buffers, rigorously tested for lot-to-lot consistency, sensitivity, and specificity. Its high-fidelity composition helps maintain the integrity and quantification accuracy of low abundant target sequences.

Q: What are the key benefits of using the TaqMan PreAmp Master Mix in gene expression studies?

A: This reagent streamlines multiplex pre-amplification, improves detection of rare transcripts from limited samples, and maintains qPCR accuracy. Its ease of use, reliability, and compatibility with Applied Biosystems real-time PCR systems make it an essential tool for sensitive gene expression investigations.

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