Thermo Fisher Scientific Applied Biosystems TaqMan Fast Advanced Master Mix for qPCR

Thermo Fisher Scientific Applied Biosystems TaqMan Fast Advanced Master Mix for qPCR

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Product Details:

Usage Type Laboratory
Features Fast cycling capability, pre-mixed convenience, formulated for reliable, reproducible results, compatible with TaqMan assays
Function Enables rapid and sensitive detection and quantitation of nucleic acid targets in real time PCR
Shelf Life 12 months (when properly stored)
Storage Instructions Store at -20C (protect from light); avoid repeat freeze-thaw cycles
Instruments Type Compatible with Applied Biosystems real-time PCR instruments
Accuracy High, optimized for reproducibility
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Thermo Fisher Scientific Applied Biosystems TaqMan Fast Advanced Master Mix for qPCR Price And Quantity

Compatible Assays Optimized for TaqMan gene expression, genotyping, and microRNA assays
Reaction Volume 1050 L per reaction (typical)
Template Types DNA or cDNA
Formulation 2X master mix (requires addition of template and primers/probes only)
Product Code Applied Biosystems 4444557 (may vary by pack size)
Enzyme Type Hot-start Taq DNA polymerase
Detection Hydrolysis probes (TaqMan)
Lot Number Information Provided on product label
Packaging Supplied in nuclease-free containers
Cycling Speed Fast protocol capable as little as 40 minutes
Regulatory Status For research use only, not for diagnostic procedures

Thermo Fisher Scientific Applied Biosystems TaqMan Fast Advanced Master Mix for qPCR Product Specifications

Noise Level Silent/Not applicable
Equipment Type Master Mix for qPCR
Use Quantitative PCR (qPCR) applications
Weight Not specified (depends on packaging size)
Power Source Not applicable (chemical reagent)
Operating Type Ready-to-use, premixed
Shelf Life 12 months (when properly stored)
Storage Instructions Store at -20C (protect from light); avoid repeat freeze-thaw cycles
Color Colorless to slightly yellow
Condition New
Function Enables rapid and sensitive detection and quantitation of nucleic acid targets in real time PCR
Features Fast cycling capability, pre-mixed convenience, formulated for reliable, reproducible results, compatible with TaqMan assays
Usage Type Laboratory
Wall Mounted No
Measurement Range Wide dynamic range for quantification
Technology TaqMan Fast qPCR
Dimension (L*W*H) Not specified (supplied in 1 mL, 5 mL, and other vial sizes)
Accuracy High, optimized for reproducibility
Material Proprietary reagent blend (including dNTPs, Taq DNA polymerase, MgCl2, and buffer)
Instruments Type Compatible with Applied Biosystems real-time PCR instruments
Portable Yes (reagent supplied in vial or bottle)
Real-Time Operation Yes

Compatible Assays Optimized for TaqMan gene expression, genotyping, and microRNA assays
Reaction Volume 1050 L per reaction (typical)
Template Types DNA or cDNA
Formulation 2X master mix (requires addition of template and primers/probes only)
Product Code Applied Biosystems 4444557 (may vary by pack size)
Enzyme Type Hot-start Taq DNA polymerase
Detection Hydrolysis probes (TaqMan)
Lot Number Information Provided on product label
Packaging Supplied in nuclease-free containers
Cycling Speed Fast protocol capable as little as 40 minutes
Regulatory Status For research use only, not for diagnostic procedures

Product Description

Brand	Thermo Fisher Scientific
Product Type	Master Mix
Pack Size	10 x 5 mL
CAT No	4444965
Application	Molecular Biology

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms. It provides best in class performance, including the widest quantifiable range, in both single and duplex reactions, even with challenging targets. TaqMan Fast Advanced Master Mix contains AmpliTaq Fast DNA Polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components. It is supplied at a 2X concentration.

Optimized for TaqMan Assays

The TaqMan Fast Advanced Master Mix is specially formulated for compatibility with a wide array of TaqMan gene expression, genotyping, and microRNA assays, ensuring robust performance and consistency across different research applications. Its proprietary blend enhances specificity and sensitivity for challenging samples.

Fast and Convenient Workflow

With its pre-mixed, ready-to-use 2X master mix format, the product streamlines qPCR preparation-just add your template and primers/probes. Rapid cycling protocols allow most reactions to be completed in as little as 40 minutes, accelerating your workflow without compromising accuracy.

Reliable Results and Broad Compatibility

Engineered for high accuracy and reproducibility, this master mix delivers dependable results, making it suitable for a wide range of quantitative real-time PCR studies. The universal compatibility with Applied Biosystems qPCR instruments ensures seamless integration into existing laboratory setups.

FAQ's of Thermo Fisher Scientific Applied Biosystems TaqMan Fast Advanced Master Mix for qPCR:

Q: How do I use the TaqMan Fast Advanced Master Mix correctly in qPCR experiments?

A: Simply add your template DNA or cDNA along with the appropriate TaqMan primers/probes to the 2X master mix. No extra preparation is needed. Follow your instrument's recommended fast cycling protocol for optimal results.

Q: What types of templates are compatible with this master mix?

A: The TaqMan Fast Advanced Master Mix is compatible with both genomic DNA and cDNA templates, making it ideal for a variety of gene expression, genotyping, and microRNA quantification applications.

Q: When should I choose this fast master mix over standard qPCR reagents?

A: Choose this master mix when you need rapid, reproducible results and a streamlined workflow. Its fast protocol reduces run times to as little as 40 minutes while maintaining sensitivity and quantitative accuracy.

Q: Where should the master mix be stored, and what are the handling precautions?

A: Store the master mix at -20C in a nuclease-free environment, protected from light. Minimize freeze-thaw cycles to preserve reagent performance and extend shelf life up to 12 months.

Q: What are the primary benefits of using this master mix in research?

A: Researchers benefit from shortened run times, reliable and precise quantification, and simplified experimental setup. Its compatibility with TaqMan assays and Applied Biosystems instruments ensures flexibility across a range of qPCR applications.

Q: Can this reagent be used for diagnostic procedures?

A: No, the TaqMan Fast Advanced Master Mix is intended for research use only and is not approved for diagnostic or clinical use.

Q: How is product identification and lot information provided?

A: Each vial or bottle is labeled with its product code and specific lot number, supporting traceability and ensuring researchers can track reagent batches as required by laboratory protocols.

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