Thermo Fisher Scientific Invitrogen SuperScript IV UniPrime One-Step RT-PCR System

Thermo Fisher Scientific Invitrogen SuperScript IV UniPrime One-Step RT-PCR System

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Product Details:

Instruments Type Molecular biology reagent system
Storage Instructions Store at -20C
Accuracy High (optimized for reproducible results)
Shelf Life 12 months (when stored as recommended)
Usage Type Laboratory Research
Measurement Range Compatible with picogram to microgram amounts of RNA
Function cDNA synthesis and amplification from RNA templates
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Thermo Fisher Scientific Invitrogen SuperScript IV UniPrime One-Step RT-PCR System Price And Quantity

Thermo Fisher Scientific Invitrogen SuperScript IV UniPrime One-Step RT-PCR System Product Specifications

Instruments Type Molecular biology reagent system
Technology SuperScript IV UniPrime technology
Storage Instructions Store at -20C
Wall Mounted No
Operating Type Automatic
Measurement Range Compatible with picogram to microgram amounts of RNA
Real-Time Operation Yes
Function cDNA synthesis and amplification from RNA templates
Accuracy High (optimized for reproducible results)
Color Standard packaging/white and blue kit
Features High sensitivity and specificity; fast reaction times; works with broad range of RNA targets
Shelf Life 12 months (when stored as recommended)
Usage Type Laboratory Research
Power Source Electricity (powered by PCR workstation)
Use Reverse transcription and PCR amplification of RNA templates in a single reaction
Condition New
Equipment Type One-Step RT-PCR System
Material High quality molecular biology grade reagents
Dimension (L*W*H) Not specified (depends on kit packaging)
Portable Yes (kit format)

Product Code 11759-500
Cycle Number Compatible with standard RT-PCR cycling programs
Application Gene expression analysis, pathogen detection, molecular diagnostics
Multiplexing Capability Yes (supports multiplex RT-PCR)
Sample Compatibility Total RNA, viral RNA, low-copy RNA templates
Reaction Format One-Step RT-PCR (single-tube protocol)
Enzyme Included SuperScript IV Reverse Transcriptase and Platinum II Taq Hot-Start DNA Polymerase
Reaction Time ~50 minutes thermal cycling process
Number of Reactions Available in various pack size (e.g., 25, 100, 500 reactions)

Product Description

Brand	Thermo Fisher Scientific
Product Type	RT-PCR Kit
Pack Size	500 x 50 micro L Reactions
CAT No	12597500
Application	Molecular Biology

Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube. It combines the high-processivity SuperScript IV Reverse Transcriptase (RT) with a novel UniPrime RT-PCR Master Mix to provide easier use and unmatched performance when compared to other one-step RT-PCR kits.

Streamlined One-Step RT-PCR Workflow

This kit enables efficient reverse transcription and PCR amplification in a single reaction tube, minimizing sample handling and reducing contamination risks. Compatible with widely-used PCR instruments, its user-friendly protocol delivers results quickly, making it suitable for time-sensitive research.

Versatile RNA Sample Compatibility

Whether working with total RNA, viral RNA, or even low-copy templates, the SuperScript IV UniPrime system ensures robust performance. Its innovative enzyme mix supports a broad input range, allowing gene expression studies and pathogen detection from diverse starting materials.

High Sensitivity and Multiplexing Capability

This system is optimized to reliably detect even challenging targets, thanks to its high sensitivity and specificity. Researchers can run multiplex RT-PCR assays, analyzing multiple RNA targets in a single reaction, which streamlines data collection and saves valuable time and reagents.

FAQ's of Thermo Fisher Scientific Invitrogen SuperScript IV UniPrime One-Step RT-PCR System:

Q: How does the SuperScript IV UniPrime One-Step RT-PCR System improve workflow efficiency?

A: By combining reverse transcription and PCR amplification in a single-tube protocol, this system reduces handling steps, saves time, and helps prevent cross-sample contamination, all while yielding rapid results in approximately 50 minutes.

Q: What types of RNA samples are compatible with this RT-PCR system?

A: The kit is compatible with total RNA, viral RNA, and low-copy RNA templates, making it ideal for various applications such as gene expression analysis, pathogen detection, and molecular diagnostics.

Q: When should I use this one-step RT-PCR system?

A: Use this system when you require quick, accurate detection and amplification of RNA, particularly for projects that demand high specificity and sensitivity, or when working with limited or precious RNA samples.

Q: Where should the reagents be stored to maintain optimal performance?

A: Reagents should be stored at -20C as recommended to ensure product integrity and maintain the kit's 12-month shelf life.

Q: What is the process for using this RT-PCR system?

A: Simply add your RNA sample to the provided reaction mix, load the tube into your thermal cycler, and follow the recommended cycling program for automatic, hands-off cDNA synthesis and amplification.

Q: Can this system support multiplex RT-PCR applications?

A: Yes, the technology is configured for multiplex RT-PCR, allowing simultaneous amplification and detection of multiple RNA targets from a single reaction, enhancing throughput and efficiency.

Q: What are the main benefits of using the SuperScript IV UniPrime One-Step RT-PCR System?

A: Benefits include high sensitivity and reliability, a streamlined workflow, reproducible results, compatibility with a broad range of RNA inputs, and fast reaction times ideal for laboratory research and molecular diagnostics.

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