Thermo Fisher Scientific Applied Biosystems Fast SYBR Green Master Mix

Thermo Fisher Scientific Applied Biosystems Fast SYBR Green Master Mix

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Product Details:

Features Fast cycling capability, high specificity, low background signal
Instruments Type Compatible with Applied Biosystems Real-Time PCR Systems
Storage Instructions Store at -20C, avoid repeated freeze-thaw cycles
Shelf Life 2 years (when stored as recommended)
Accuracy High sensitivity and specificity for DNA detection
Measurement Range Wide dynamic range suitable for most real-time PCR assays
Usage Type Laboratory reagent
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Thermo Fisher Scientific Applied Biosystems Fast SYBR Green Master Mix Price And Quantity

Thermo Fisher Scientific Applied Biosystems Fast SYBR Green Master Mix Product Specifications

Wall Mounted No
Use DNA Quantification and Detection in Real-Time PCR
Material Proprietary chemical blend with SYBR Green dye
Technology qPCR (Real-Time Quantitative PCR)
Features Fast cycling capability, high specificity, low background signal
Storage Instructions Store at -20C, avoid repeated freeze-thaw cycles
Instruments Type Compatible with Applied Biosystems Real-Time PCR Systems
Condition New
Real-Time Operation Yes
Equipment Type Real-Time PCR Master Mix
Shelf Life 2 years (when stored as recommended)
Portable Yes, requires compatible qPCR instrument
Accuracy High sensitivity and specificity for DNA detection
Color Green (fluorescent dye)
Measurement Range Wide dynamic range suitable for most real-time PCR assays
Operating Type Automated
Usage Type Laboratory reagent
Function DNA amplification and detection

Buffer Components Includes dNTPs, MgCl, optimized buffer system
Enzyme Fast DNA Polymerase
Reaction Volume 1050 L per reaction
Packaging Size Various sizes available (e.g., 1 mL, 5 mL, 25 mL)
Format 2X Master Mix
Compatibility Optimized for Fast cycling protocols and Applied Biosystems 7500 Fast Real-Time PCR System
Dye SYBR Green I
Product Code 4385612
Application Gene expression analysis, SNP genotyping, pathogen detection

Product Description

Brand	Thermo Fisher Scientific
Product Type	Master Mix
Pack Size	10 x 5 mL
CAT No	4385618
Application	Molecular Biology

Fast SYBRa Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for your real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency.

Rapid, Sensitive, and Reliable qPCR Performance

Applied Biosystems Fast SYBR Green Master Mix offers researchers the advantage of quick PCR run times while maintaining high accuracy in DNA quantification and detection. The optimized formulation enables fast cycling without compromising sensitivity or specificity, resulting in reliable gene analysis. The master mix is perfectly matched for the Applied Biosystems 7500 Fast Real-Time PCR System, streamlining your workflow for gene expression studies, SNP analysis, and pathogen testing in molecular biology labs.

Ready-to-Use Master Mix for Fast Workflow

With its 2X concentration, inclusion of all necessary buffer components, and easy protocol, this master mix simplifies qPCR setup and reduces the chance of error. Simply add your template and primers, and you're ready to amplify and detect DNA sequences with precision. The blend also minimizes background signals for clearer results, making it an excellent choice for both routine and advanced laboratory applications.

FAQ's of Thermo Fisher Scientific Applied Biosystems Fast SYBR Green Master Mix:

Q: How do I use the Fast SYBR Green Master Mix in my qPCR assays?

A: To use this master mix, simply combine it (at 2X concentration) with your DNA template, primers, and nuclease-free water. Initiate the qPCR protocol on a compatible instrument like the Applied Biosystems 7500 Fast Real-Time PCR System, and follow your gene target's recommended cycling conditions.

Q: What types of applications is this master mix suitable for?

A: The Fast SYBR Green Master Mix is ideal for gene expression analysis, SNP genotyping, and pathogen detection, providing fast and accurate results for a wide variety of real-time PCR assays.

Q: When should I use this master mix instead of a standard qPCR reagent?

A: You should choose this master mix when you require shorter run times without compromising assay sensitivity or specificity, especially if you use a fast-capable qPCR instrument like the Applied Biosystems 7500 Fast Real-Time PCR System.

Q: Where should I store the Fast SYBR Green Master Mix?

A: Store the master mix at -20C to maintain its stability and effectiveness. Avoid repeated freeze-thaw cycles to ensure optimal performance over its 2-year shelf life.

Q: What are the benefits of using the Fast SYBR Green Master Mix for my laboratory?

A: This master mix provides a ready-to-use solution for fast, accurate, and sensitive DNA detection, saving time and reducing pipetting errors while producing reliable, high-throughput results for diverse research needs.

Q: Does the master mix work with non-Applied Biosystems qPCR instruments?

A: While optimized for Applied Biosystems Real-Time PCR Systems, it may function on other fast-capable qPCR platforms. However, optimal results and manufacturer support are guaranteed with Applied Biosystems systems.

Q: What precautions should I take when handling and storing the mix?

A: Keep the reagent protected from light, store at -20C, and avoid multiple freeze-thaw cycles. Always wear appropriate gloves and lab safety gear while handling qPCR reagents.

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