Thermo Fisher Scientific Invitrogen PureLink RNA Micro Scale Kit

Thermo Fisher Scientific Invitrogen PureLink RNA Micro Scale Kit

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Product Details:

Instruments Type Molecular Biology Reagent Kit
Accuracy High RNA yield and purity for downstream applications
Storage Instructions Store at room temperature (some components may require 4C)
Usage Type Laboratory
Function Extraction, purification of total RNA
Measurement Range 15,000 ng RNA input per preparation
Features High recovery, DNA-free RNA, suited for low input samples, fast protocol
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Thermo Fisher Scientific Invitrogen PureLink RNA Micro Scale Kit Price And Quantity

Thermo Fisher Scientific Invitrogen PureLink RNA Micro Scale Kit Product Specifications

Features High recovery, DNA-free RNA, suited for low input samples, fast protocol
Shelf Life 12 months (reagents unopened at recommended storage)
Power Source Not required (manual protocol)
Technology Silica membrane-based nucleic acid purification
Real-Time Operation No
Equipment Type RNA Isolation Kit
Function Extraction, purification of total RNA
Measurement Range 15,000 ng RNA input per preparation
Material Plastic (spin columns, collection tubes), Reagents (Buffers, Enzymes)
Color Multi-color (as per kit contents)
Accuracy High RNA yield and purity for downstream applications
Wall Mounted No
Operating Type Manual
Use For extraction and purification of total RNA from micro-scale samples
Condition New
Portable Yes
Storage Instructions Store at room temperature (some components may require 4C)
Usage Type Laboratory
Instruments Type Molecular Biology Reagent Kit

Product Description

Brand	Thermo Fisher Scientific
Product Type	RNA Kit
Pack Size	50 Preps
CAT No	12183016
Product Class	Molecular Biology

The PureLink RNA Micro Scale Kit provides rapid purification and concentration of total RNA from a variety of samples (e.g., LCM tissues, fine needle aspirates, and FACS-sorted cells).

Efficient RNA Isolation from Minimal Samples

Engineered for micro-scale RNA extraction, the PureLink RNA Micro Scale Kit excels in recovering high-quality RNA from samples as limited as 10 cells or tiny tissue fragments. Utilizing advanced silica membrane columns, the protocol ensures reliable and reproducible yields, making it an ideal choice for challenging low-input experiments or precious clinical materials.

Integrated On-Column DNase Treatment

The kit incorporates on-column DNase digestion, ensuring removal of genomic DNA without extra handling. This feature streamlines the workflow and reduces the risk of contamination, resulting in RNA preparations suitable for highly sensitive downstream analyses such as qRT-PCR and RNA-Seq.

Flexible and Fast Workflow

Requiring only a typical lab microcentrifuge, this kit processes samples in about 30 minutes. Its manual format and compact design mean it is easily portable and can fit into any laboratory setup in India. The high recovery rates and purity eliminate the need for repeat extractions, optimizing both time and sample use.

FAQ's of Thermo Fisher Scientific Invitrogen PureLink RNA Micro Scale Kit:

Q: How does the PureLink RNA Micro Scale Kit ensure high-quality RNA from very small samples?

A: The kit uses a silica membrane spin column technology that efficiently binds and purifies RNA, even from micro-scale inputs such as 10 cells. The integrated on-column DNase ensures DNA-free recovery, maximizing RNA yield and purity for critical downstream applications.

Q: What types of samples can be processed with this kit?

A: This kit is suitable for micro-scale samples, including cultured cells (as few as 10), tissue specimens, and material from laser-capture microdissection. It supports a wide RNA input range of 1-5,000 ng per extraction.

Q: When should I use DNase treatment included in the kit?

A: The on-column DNase step is recommended for all preparations to eliminate genomic DNA contamination, especially when preparing RNA for sensitive techniques like qRT-PCR or RNA-Seq. The kit conveniently includes all necessary DNase reagents.

Q: Where can this kit be used in the laboratory workflow?

A: The PureLink RNA Micro Scale Kit fits seamlessly into standard molecular biology workflows. It is compatible with commonly available microcentrifuges (1.5-2 mL tubes) and does not require any power source, making it versatile and portable in laboratory settings.

Q: What is the typical recovery and elution volume for RNA extraction using this kit?

A: The kit delivers maximum possible RNA recovery from micro-scale samples, with a typical elution volume of 10-15 L, suitable for downstream applications demanding high concentration and purity.

Q: How long does the RNA extraction process take with this kit?

A: A complete RNA extraction and purification, including on-column DNase treatment, typically takes around 30 minutes using this kit, allowing for efficient sample processing and minimal turnaround time.

Q: What are the main benefits of using the PureLink RNA Micro Scale Kit?

A: Key benefits include high recovery rates from limited samples, DNA-free RNA output, compatibility with high-sensitivity applications, no power requirement, and a straightforward, fast protocol ideal for laboratories seeking reliable results with minimal sample input.

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