Product Description
Brand InBios
Sample Type Serum
Pack Size 96 Wells
CAT No STGS-1
The Scrub Typhus Detect IgG ELISA test for exposure to Orientia tsutsugamushi (OT; formerly Rickettsia) is an ELISA assay system for the detection of IgG antibodies in human serum to OT-derived recombinant antigen. This test is to aid in the diagnosis of human exposure to OT species. It is not intended to screen blood or blood components, and is for in vitro diagnostic use only. The complete kit includes one 96 well strippable plate with all necessary reagents and controls.
Reliable Serological DiagnosisThe InBios Scrub Typhus Detect IgG ELISA Kit provides laboratories with an accurate and efficient solution for the detection of IgG antibodies against Orientia tsutsugamushi. Featuring high sensitivity and specificity, this kit helps improve diagnostic confidence, supporting timely and informed clinical decisions for scrub typhus cases.
User-Friendly and Ready-to-UseAll reagents are supplied ready-to-use or require minimal preparation, and the kit follows a straightforward, easy-to-follow protocol. With color-coded components and included positive and negative controls, users can expect streamlined testing with precise and reproducible results.
Versatile and Convenient for LaboratoriesCompatible with human serum or plasma samples, this kit provides qualitative and semi-quantitative results in about two hours. Its standard 96-well format is suitable for most laboratory workflows, and the kit's transportability and storage requirements (refrigerated at 2-8C) enhance its practicality in diverse diagnostic settings.
FAQ's of InBios Scrub Typhus Detect IgG ELISA Kit:
Q: How is the Scrub Typhus Detect IgG ELISA Kit used in the laboratory?
A: The kit is used to detect IgG antibodies to Orientia tsutsugamushi in human serum or plasma. After adding samples and controls to the coated microtiter plate, reagents are added sequentially following the provided protocol. Color development, measured at 450 nm, indicates the presence of antibodies.
Q: What sample types are compatible with this ELISA kit?
A: It is validated for use with human serum or plasma samples. Ensure samples are handled and processed as outlined in the kit insert for optimal results.
Q: When should this kit be used in the diagnosis process?
A: The kit should be used when scrub typhus infection is suspected, typically following clinical presentation and initial screening. It is most effective in detecting IgG antibodies during both early and later phases of infection, depending on the individual's immune response.
Q: Where should the kit be stored and what are the storage requirements?
A: Store the kit at 2-8C as recommended by the manufacturer. Do not freeze any of the components. This storage condition helps maintain reagent stability for the full 12-18 month shelf life.
Q: What are the benefits of using this ELISA kit?
A: This kit offers high sensitivity and specificity for reliable results, includes all necessary controls, and provides qualitative as well as semi-quantitative data. Its straightforward workflow and ready-to-use reagents enhance efficiency, making it ideal for diagnostic laboratories.
Q: What controls are included and why are they important?
A: Positive and negative controls are included with each kit, ensuring test validity and helping to confirm the accuracy of the results during each assay run.
Q: How long does the assay take and what equipment is required?
A: The complete assay takes about two hours and is performed at room temperature (20-25C) unless specified otherwise. Standard ELISA equipment, including a spectrophotometer capable of reading at 450 nm, is required.