Qiagen TAGZyme DAPase Enzyme (50 U)

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Product Details:

Instruments Type Biochemical reagent
Storage Instructions Store at -20C; avoid repeated freeze/thaw cycles
Usage Type Single use or multi-use based on required units
Features High specificity and activity; optimized for TAGZyme buffers
Function DAPase selectively removes diaminopurine (DAP)
Measurement Range 50 Units (as per vial)
Shelf Life 1 year (if stored correctly)
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Qiagen TAGZyme DAPase Enzyme (50 U) Price And Quantity

Unit Definition 1 Unit catalyzes the conversion of 1 mol DAP per minute under standard conditions
Enzyme Source Recombinant, E. coli
Catalog Number 1078597
Buffer Composition Supplied in TAGZyme storage buffer
Hazard Information Non-hazardous according to GHS criteria
Application Used in high-throughput oligonucleotide purification systems
Pack Size 50 Purification Units per vial
Product Name Qiagen TAGZyme DAPase Enzyme (50 U)
Form Liquid

Qiagen TAGZyme DAPase Enzyme (50 U) Product Specifications

Features High specificity and activity; optimized for TAGZyme buffers
Operating Type Laboratory manual use
Storage Instructions Store at -20C; avoid repeated freeze/thaw cycles
Usage Type Single use or multi-use based on required units
Instruments Type Biochemical reagent
Accuracy High, product purity ensured
Measurement Range 50 Units (as per vial)
Shelf Life 1 year (if stored correctly)
Function DAPase selectively removes diaminopurine (DAP)
Use For enzymatic removal of DAP during oligonucleotide synthesis
Material Biological (recombinant enzyme in buffer)
Wall Mounted No
Portable Yes (supplied in microcentrifuge tube)
Equipment Type Enzyme Solution
Technology TAGZyme Removal System
Condition New
Real-Time Operation No
Color Clear

Unit Definition 1 Unit catalyzes the conversion of 1 mol DAP per minute under standard conditions
Enzyme Source Recombinant, E. coli
Catalog Number 1078597
Buffer Composition Supplied in TAGZyme storage buffer
Hazard Information Non-hazardous according to GHS criteria
Application Used in high-throughput oligonucleotide purification systems
Pack Size 50 Purification Units per vial
Product Name Qiagen TAGZyme DAPase Enzyme (50 U)
Form Liquid

Product Description

Brand Qiagen

Product Type Enzyme Kit

Pack Size 50 U

CAT No 34366

Application Molecular Reagent

The TAGZyme Enzyme DAPase includes sufficient enzyme for highly specific and efficient His-tag removal from up to 10 mg His-tagged protein. The TAGZyme system can be used for His-tag removal from proteins containing an intrinsic DAPase stop point expressed using the TAGZyme pQE-2 vector.

Efficient DAP Removal for Oligonucleotide Purification

The Qiagen TAGZyme DAPase Enzyme (50 U) is meticulously designed for selective removal of diaminopurine in high-throughput oligonucleotide synthesis workflows. Its recombinant origin ensures consistent performance and high activity, making it ideal for laboratories seeking precise purification results. Compatible with the TAGZyme system, it streamlines enzymatic clean-up processes, improving the quality and yield of synthetic oligonucleotides.

Optimized Storage and Handling

Supplied as a clear liquid in a microcentrifuge tube, the enzyme remains stable for up to one year if stored at -20C. Proper storage prevents loss of activity due to repeated freeze/thaw cycles, ensuring reliable results throughout its shelf life. The portable packaging supports operational flexibility across different lab setups.

FAQ's of Qiagen TAGZyme DAPase Enzyme (50 U):

Q: How does the Qiagen TAGZyme DAPase Enzyme function in oligonucleotide purification?

A: This enzyme catalyzes the removal of diaminopurine (DAP) from oligonucleotides, facilitating clean and efficient purification during synthetic workflows. Its high specificity targets DAP residues, ensuring precise enzymatic action.

Q: What is the proper way to store the Qiagen TAGZyme DAPase Enzyme (50 U) to maintain activity?

A: Store the enzyme at -20C and avoid multiple freeze/thaw cycles. Following these instructions ensures up to one year of shelf life with preserved performance.

Q: When should I use this enzyme during oligonucleotide synthesis?

A: The DAPase enzyme is applied during the oligonucleotide purification phase, after synthesis, to enzymatically remove DAP and achieve the desired purity in final products.

Q: Where can the Qiagen TAGZyme DAPase Enzyme be used?

A: This product is optimized for laboratory use in high-throughput oligonucleotide purification systems, making it suitable for research, diagnostic, and biotechnological laboratories.

Q: What are the benefits of using a recombinant enzyme from E. coli in this application?

A: Recombinant production in E. coli ensures batch-to-batch consistency, high purity, and reliable specificity, all of which are critical for reproducible laboratory results.

Q: How many purifications can be performed with one vial?

A: Each vial contains 50 purification units. The number of reactions depends on the number of units required per purification; refer to your protocol for precise calculations.

Q: Is the enzyme hazardous, and what safety precautions should be followed?

A: The enzyme is classified as non-hazardous according to GHS criteria. Standard laboratory safety protocols, such as wearing gloves and protective eyewear, are nevertheless recommended during handling.

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