Product Description
Brand BioRad
Model QX ONE
Capacity 96 Wells
CAT No 12006536
Application Molecular Biology
The QX ONE ddPCR System is designed to deliver a precise and multiplexed digital PCR system. This system seamlessly integrates a standard ddPCR workflow of droplet generation, thermal cycling, droplet reading, and analysis into a hands-free precision platform.
Advanced Multiplexing CapabilitiesWith five independent fluorescence channels, the QX ONE enables simultaneous detection and quantification of multiple targets within the same sample. This multiplexing power facilitates efficient genetic analysis, disease research, and biomarker validation, delivering high-quality results in one run and increasing throughput in demanding laboratory environments.
Automated Workflows for Higher ThroughputThe fully walk-away operational design streamlines every step, from droplet generation and PCR amplification to droplet reading. Processing up to four 96-well plates per run, the integrated plate handler and touchscreen interface minimize manual handling, making the QX ONE well-suited for laboratories that require rapid and consistent sample processing.
FAQ's of BioRad QX ONE Droplet Digital PCR (ddPCR) System:
Q: How does the BioRad QX ONE ddPCR System automate the workflow in the laboratory?
A: The QX ONE ddPCR System provides a fully automated process, including droplet generation, PCR cycling, and droplet reading. Its integrated plate handler and touchscreen interface reduce manual intervention, allowing researchers to set up runs and walk away while the instrument completes all necessary steps.
Q: What types of consumables are compatible with the QX ONE ddPCR System?
A: This ddPCR System is compatible with BioRad ddPCR Supermixes and standard 96-well plates. These consumables ensure optimal reaction performance and accurate digital quantification across a broad range of applications.
Q: When should researchers utilize the QX ONE ddPCR System for their experiments?
A: Researchers should use the QX ONE for experiments requiring high sensitivity, precision, and absolute quantification of DNA or RNA targets, especially where multiplexing and minimized hands-on time are critical, such as clinical diagnostics, genomics, and biomarker studies.
Q: Where can the BioRad QX ONE ddPCR System be installed within a laboratory?
A: The system is designed for bench-top use in controlled laboratory environments. Its dimensions (57 75 70 cm) and weight (approximately 140 kg) require a stable surface, away from excess moisture, heat, or direct sunlight.
Q: What benefits does multiplexing provide in ddPCR assays?
A: Multiplexing allows simultaneous quantification and differentiation of up to five genetic targets per reaction, saving time, reducing reagent consumption, and increasing data on each sample. This capability is particularly advantageous in complex studies or clinical applications.
Q: How accurate is the QX ONE ddPCR System for nucleic acid quantification?
A: The system offers highly accurate absolute quantification, capable of detecting down to 1 copy/L, with results directly calculated from up to 100,000 droplets per well. This accuracy ensures reliable data for applications such as rare mutation detection or viral load monitoring.
Q: What is the recommended storage condition for the BioRad QX ONE ddPCR System?
A: It is advisable to keep the instrument in a controlled laboratory environment, maintaining stable temperature and humidity levels, and protecting it from dust and corrosive substances to optimize performance and longevity.