Thermo Fisher Scientific Invitrogen PureLink Total RNA Blood Kit

Thermo Fisher Scientific Invitrogen PureLink Total RNA Blood Kit

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Product Details:

Measurement Range Works with 0.12.5 mL of whole blood per prep
Storage Instructions Store at room temperature (1525C); some components may require refrigerated storage
Usage Type Laboratory/Research
Accuracy High recovery, yield dependent on sample input
Shelf Life 12-24 months (when stored as recommended)
Instruments Type Nucleic Acid Purification Kit
Function Total RNA extraction and purification
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Thermo Fisher Scientific Invitrogen PureLink Total RNA Blood Kit Price And Quantity

Thermo Fisher Scientific Invitrogen PureLink Total RNA Blood Kit Product Specifications

Shelf Life 12-24 months (when stored as recommended)
Portable Yes
Instruments Type Nucleic Acid Purification Kit
Use Isolation and purification of high-quality total RNA from blood
Material Plastic consumables and reagents for nucleic acid purification
Function Total RNA extraction and purification
Equipment Type RNA Extraction Kit
Features High purity, ready-to-use RNA, efficient removal of contaminants
Color White and blue (as per packaging)
Wall Mounted No
Operating Type Manual
Condition New
Technology Silica-membrane spin column purification
Measurement Range Works with 0.12.5 mL of whole blood per prep
Storage Instructions Store at room temperature (1525C); some components may require refrigerated storage
Real-Time Operation No
Usage Type Laboratory/Research
Accuracy High recovery, yield dependent on sample input

Product Description

Brand	Thermo Fisher Scientific
Product Type	Total RNA Blood Kit
Pack Size	50 Preps
CAT No	K156001
Application	Molecular Biology

PureLink Total RNA Blood Kit allows purification of high-quality total RNA from 50 - 500 I14l fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol.
a RNA purification from whole blood in under an hour
a Yields high-quality RNA devoid of non-nucleated erythrocytes.

Efficient Manual RNA Isolation

This kit offers a simple, manual protocol optimized for isolating total RNA from human whole blood originating from EDTA, citrate, or heparin tubes. The silica-membrane spin column technology allows for high-yield, high-purity RNA, minimizing contaminants and inhibitors for accurate downstream molecular applications.

Flexible and User-Friendly Design

Each kit comes with pre-packed spin columns, collection tubes, buffers, and RNase-free water, ensuring a ready-to-use workflow. The process is straightforward and can be completed in under 60 minutes per sample, making it highly suited for both low- and medium-throughput research laboratories.

FAQ's of Thermo Fisher Scientific Invitrogen PureLink Total RNA Blood Kit:

Q: How does the PureLink Total RNA Blood Kit extract RNA from blood samples?

A: The kit utilizes a silica-membrane spin column purification method, which selectively binds RNA from lysed blood samples. Impurities and contaminants are efficiently washed away, while high-quality total RNA is eluted in RNase-free water for downstream use.

Q: What types of blood samples are compatible with this RNA extraction kit?

A: The kit is compatible with both fresh and stabilized human blood samples, including those collected in EDTA, citrate, or heparin tubes, making it versatile for various laboratory sample types.

Q: When should DNase treatment be used during the extraction process?

A: DNase treatment is optional and should be included during the protocol if removal of genomic DNA is required for sensitive downstream applications such as RT-qPCR or RNA sequencing.

Q: Where should the kit and its reagents be stored?

A: Most components can be stored at room temperature (15-25C). If the kit contains enzymes or sensitive reagents, these should be stored refrigerated as detailed in the kit's instruction manual.

Q: What is the typical processing time for isolating RNA with this kit?

A: Each sample can be processed in less than 60 minutes, allowing for quick RNA extraction and purification in manual, individual workflows.

Q: How much blood can be processed with a single preparation?

A: The kit supports extraction from 0.1 to 2.5 mL of whole blood per preparation, accommodating different sample sizes and research requirements.

Q: What are the main benefits of using this kit for RNA extraction?

A: The PureLink kit allows for efficient removal of contaminants and inhibitors, delivers high-purity, ready-to-use RNA, and is suitable for various molecular biology applications, ensuring reliable and reproducible results in laboratory research.

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