Product Description
| Brand | CTK Biotech-Athenese Dx |
| Sample Type | Serum/ Plasma |
| Pack Size | 96 Wells |
| CAT No | AE0420 |
The TRUSTwell HIV Ag-Ab ELISA Kit is a solid phase enzyme linked immunoabsorbent assay for the qualitative detection of HIV-1 P24 antigen, anti-HIV-1 including subtype O and anti-HIV-2 antibodies (including isotype IgG, IgM and IgA) in human serum or plasma.
Comprehensive and Accurate HIV DetectionThe TRUSTwell HIV Ag-Ab ELISA detects both HIV-1 p24 antigen and antibodies to HIV-1 and HIV-2, enabling identification of early and established HIV infections. Its high sensitivity and specificity (>99%) make it an excellent choice for clinical laboratories aiming to confidently screen and diagnose HIV infections. The inclusion of all necessary reagents and controls ensures convenience and reliability in each run.
User-Friendly Design and WorkflowDesigned for manual operation, the kit is straightforward to use in standard laboratory environments. With all reagents pre-prepared, the workflow reduces hands-on time and potential pipetting errors. The process can be completed in about 60-120 minutes, with easy-to-interpret, colorimetric results, either visually or using a plate reader at 450/630nm.
Quality, Safety, and StorageEvery kit undergoes stringent quality checks and features a 12-month shelf life, provided it is stored at 2-8C. The clearly labeled expiry date and robust kit construction ensure product integrity. This allows reliable performance from the first to the last assay, supporting regular single-use testing in clinical settings.
FAQ's of CTK Biotech TRUSTwell HIV Ag-Ab ELISA:
Q: How does the TRUSTwell HIV Ag-Ab ELISA detect HIV infection?
A: The kit utilizes a 4th generation indirect sandwich ELISA format to qualitatively detect HIV-1 p24 antigen and HIV-1/2 antibodies in human serum or plasma, allowing for early and accurate identification of HIV infection.
Q: What sample types can be used with this ELISA kit?
A: You can use either human serum or plasma samples collected from individuals for testing with the TRUSTwell HIV Ag-Ab ELISA kit.
Q: What is the recommended process for performing the assay?
A: To perform the assay, add the sample and controls to the microtiter wells, incubate with the provided reagents for 60-120 minutes, wash as instructed, add substrate and stop solution, and then interpret the results visually by color change or using a plate reader at 450/630nm.
Q: When are results considered reactive or non-reactive?
A: Results are categorized as reactive or non-reactive based on the optical density (OD) value measured after completion of the assay. The kit includes positive, negative, and cut-off controls to guide interpretation.
Q: Where should the kit be stored for optimal performance?
A: Store the kit at a temperature between 2-8C to maintain the reliability and shelf life of the reagents, as specified by the manufacturer.
Q: What are the main benefits of using this ELISA kit for HIV screening?
A: Key benefits include its high sensitivity and specificity (>99%), ease of use with ready-to-use reagents, fast protocol (results within 2 hours), and reliable controls to ensure accurate interpretation.
Q: Can this kit be used for real-time operation or home testing?
A: No, the TRUSTwell HIV Ag-Ab ELISA kit is intended for manual use by trained professionals in clinical laboratory environments and is not designed for real-time or home-based testing.