Thermo Fisher Applied Biosystems TaqPath 1-Step Multiplex Master Mix (No ROX)

Thermo Fisher Applied Biosystems TaqPath 1-Step Multiplex Master Mix (No ROX)

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Product Details:

Material Proprietary chemical formulation, typically supplied in plastic vials
Condition New
Technology 1-Step RT-qPCR (Multiplex) Chemistry
Disposable No, intended for single use per assay
Software Detail Requires compatible real-time PCR analysis software
Measurement Suitable for RNA and DNA template quantification
Synchronize With Real-time PCR instruments (excluding those requiring ROX reference dye)
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Thermo Fisher Applied Biosystems TaqPath 1-Step Multiplex Master Mix (No ROX) Price And Quantity

Thermo Fisher Applied Biosystems TaqPath 1-Step Multiplex Master Mix (No ROX) Product Specifications

Color Colorless solution
Material Proprietary chemical formulation, typically supplied in plastic vials
Attributes No ROX reference dye; optimized for high specificity and sensitivity in multiplexing; compatible with probe-based detection systems
Measurement Suitable for RNA and DNA template quantification
Technology 1-Step RT-qPCR (Multiplex) Chemistry
Weight Kit-dependent, minimal (reagent vial)
Synchronize With Real-time PCR instruments (excluding those requiring ROX reference dye)
Software Detail Requires compatible real-time PCR analysis software
Real-Time Operation Yes, compatible with real-time PCR applications
Size Provided in 1 mL or multiple kit sizes
Condition New
Disposable No, intended for single use per assay
Operating Type In vitro diagnostic (IVD) & research use only
Usage For detection and quantitation of RNA targets using multiplex one-step RT-qPCR

Product Description

Brand	Thermo Fisher Scientific
Product Type	RT-qPCR Mix
Pack Size	1 x 0.5 mL
CAT No	A28521
Application	Molecular Biology

TaqPath 1-Step Multiplex Master Mix (No ROX) is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction. Particularly useful for diagnostics and biopharmaceuticals with applications in viral pathogen detection and high throughput gene expression workflows, the mix includes thermostable MMLV reverse transcriptase, dNTPs, UNG, and thermostable Fast DNA polymerase all in a single tube. TaqPath is manufactured under a strong ISO13485 quality management system and is designed to produce consistent product performance lot after lot.

Optimized for Multiplex RT-qPCR

TaqPath 1-Step Multiplex Master Mix (No ROX) is specifically developed for simultaneous detection of up to four RNA targets in one reaction, making it highly suitable for viral and pathogen screening, as well as gene expression studies. Its proprietary formulation ensures exceptional sensitivity and specificity, enabling researchers and clinicians to capture low-abundance targets efficiently.

Streamlined Workflow and Enhanced Inhibition Resistance

Experience a simplified reaction setup at room temperature, removing the need for ice during assembly. The master mix's high tolerance to common PCR inhibitors ensures reliable performance even in complex sample matrices, such as blood or swab extracts, elevating confidence in your results.

Flexible Application and Broad Compatibility

Designed for use with a wide range of real-time PCR instruments that do not require ROX passive reference dye, this master mix can be integrated into workflows across research, clinical, and diagnostic laboratories. Its adaptation for both in vitro diagnostic and research use enhances its versatility for different testing scenarios.

FAQ's of Thermo Fisher Applied Biosystems TaqPath 1-Step Multiplex Master Mix (No ROX):

Q: How do I set up reactions using the TaqPath 1-Step Multiplex Master Mix (No ROX)?

A: Setting up a reaction is straightforward-combine your RNA template, gene-specific primers/probes, and the 2X master mix in a single tube. Thanks to its unique formulation, the setup can be done at room temperature, and you can proceed directly to your real-time PCR instrument.

Q: What types of applications is this master mix intended for?

A: The master mix is ideal for multiplex real-time RT-qPCR, including the detection and quantification of viral RNA, pathogen screening, and gene expression analysis. It is suitable for both in vitro diagnostic (IVD) and research applications.

Q: When should I use this master mix instead of others with a ROX reference dye?

A: Use this mix with real-time PCR instruments that do not require a ROX passive reference dye as a normalization control. It is optimized for high specificity and sensitivity in probe-based multiplex assays where ROX is unnecessary.

Q: Where should I store the TaqPath 1-Step Multiplex Master Mix for optimal stability?

A: Store the master mix at -20C for long-term stability. For transport, it is shipped on dry ice to ensure product integrity until it reaches your lab.

Q: What are the benefits of multiplexing capability in this master mix?

A: Multiplexing allows you to detect up to four targets per reaction, saving both time and resources. This capability enables simultaneous pathogen identification or gene expression profiling from a single sample, increasing throughput and data efficiency.

Q: How resistant is this master mix to PCR inhibitors found in clinical samples?

A: The master mix is formulated for high tolerance to common PCR inhibitors, such as those found in blood, tissue, and environmental samples, ensuring reliable results even with challenging sample types.

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