Product Description
Brand BioRad
Product Type Protein Kit
Pack Size 20 Prep
CAT No 1632086
Application Molecular Biology
The ReadyPrep protein extraction kit (total protein) provides a simple, rapid, and reproducible method for preparation of total cellular protein extracts from a wide variety of biological samples. Use of this kit generates protein samples that can be applied directly to a variety of applications, including IEF and 2-D gel separation.
Efficient Protein Extraction for ResearchBenefit from rapid preparation of total protein extracts from biological samples with the BioRad ReadyPrep Protein Extraction Kit. Designed for laboratories, this kit streamlines workflows and ensures reproducible results whether you're working with mammalian tissues or cultured cells. Its ready-to-use reagents and compatibility with multiple applications make it indispensable for protein studies.
Broad Sample CompatibilityThis kit supports extraction from a wide variety of mammalian tissues and cultured cells, accommodating up to 100 mg of tissue or 1 x 10^7 cells per extraction. The versatility allows researchers to address multiple experimental needs using a single kit, optimizing sample processing and analysis.
Reliable Performance and ConvenienceFeaturing a quick, manual process with less than 60 minutes hands-on time, the ReadyPrep kit is ideal for efficient laboratory work. The boxed packaging keeps components organized, while the straightforward instructions facilitate streamlined usage regardless of your experience level.
FAQ's of BioRad ReadyPrep Protein Extraction Kit (Total Protein):
Q: How do I use the BioRad ReadyPrep Protein Extraction Kit for protein isolation?
A: Begin by preparing your biological sample (up to 100 mg tissue or 1 x 10^7 cells), then follow the instructions booklet to add the extraction reagent, perform washing steps, and neutralize the extract. The process is manual and typically completed in under 60 minutes.
Q: What types of samples can be processed with this kit?
A: The kit is compatible with mammalian tissues and cultured cells, making it suitable for most laboratory-based protein extraction protocols in research or diagnostic settings.
Q: When should I use the ReadyPrep Protein Extraction Kit in my workflow?
A: Utilize this kit when you need reliable and rapid extraction of total proteins from biological samples for downstream analyses such as SDS-PAGE, western blotting, 2D electrophoresis, or protein quantitation.
Q: Where should I store the kit to maintain its stability?
A: Store all components at 2-8C as recommended on the label to preserve their efficacy. Always refer to the product instructions for specific storage details.
Q: What is the benefit of using this kit compared to traditional protein extraction methods?
A: This kit offers ready-to-use reagents, short processing time, and consistent protein yields, reducing experimental variability and saving valuable laboratory time.
Q: What downstream applications are compatible with the protein extracts from this kit?
A: The extracted proteins can be used with SDS-PAGE, western blotting, 2D gel electrophoresis, and most standard protein quantitation assays.
Q: Are there any safety considerations for handling the kit reagents?
A: Some reagents may be potentially hazardous; consult the MSDS for each component before use and follow appropriate laboratory safety protocols.