Qiagen RNeasy Mini QIAcube Kit (240)

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Product Details:

Shelf Life 1224 months (refer to label)
Instruments Type Consumables for automated nucleic acid purification
Function Isolates high-integrity total RNA suitable for sensitive applications
Measurement Range Processes samples up to 1x10^7 cells or 30 mg tissue per prep
Accuracy High yield and purity of RNA
Storage Instructions Store at room temperature (1525C); certain buffers may require refrigeration upon opening
Usage Type Laboratory
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Qiagen RNeasy Mini QIAcube Kit (240) Price And Quantity

Qiagen RNeasy Mini QIAcube Kit (240) Product Specifications

Measurement Range Processes samples up to 1x10^7 cells or 30 mg tissue per prep
Wall Mounted No
Technology Silica membrane-based RNA purification technology
Function Isolates high-integrity total RNA suitable for sensitive applications
Instruments Type Consumables for automated nucleic acid purification
Material High-quality plastic components, silica membrane spin columns, buffers
Color White (kit box and components)
Dimension (L*W*H) Kit box approximately 21 19 16 cm
Condition New
Weight ~2 kg (kit)
Shelf Life 1224 months (refer to label)
Portable Yes (kit only, for lab transport)
Use Purification of total RNA from cells and tissues
Real-Time Operation Compatible with automated platforms like QIAcube
Power Source N/A (requires use of QIAcube instrument)
Power Consumption Dependent on QIAcube instrument
Features Automated RNA extraction, high purity RNA, ready for use with QIAcube, ready-to-use reagents
Operating Type Automated and Manual
Voltage Dependent on QIAcube instrument
Usage Type Laboratory
Accuracy High yield and purity of RNA
Storage Instructions Store at room temperature (1525C); certain buffers may require refrigeration upon opening
Equipment Type Nucleic Acid Purification Kit

Product Description

Brand Qiagen

Product Type RNA Kit

Pack Size 240T

CAT No 74116

Application Molecular Reagent

RNeasy Kits are the gold standard for total RNA isolation. They provide fast purification of high-quality RNA from small to large amounts of cells, tissues, and yeast using silica membrane RNeasy spin columns or 96-well plates. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor II or TissueLyser II or LT system. The RNeasy 96 Kit enables high-throughput purification of total RNA from up to 96 cultured cell samples using silica membrane RNeasy 96 plates. A dedicated RNeasy QIAcube Kit enables automated purification of 112 samples on theQIAcube Connect.

Automated, High-Yield RNA Purification

This kit streamlines the process of isolating total RNA from diverse biological samples, ensuring high yields and purity suitable for the most sensitive downstream applications. The integration with QIAcube platforms allows for automation of routine tasks, improving reliability and reducing hands-on time, making it ideal for laboratories seeking efficiency and consistency.

Comprehensive Kit Components for Lab Convenience

The RNeasy Mini QIAcube Kit includes premeasured, ready-to-use lysis and wash buffers, RNase-free water, specialized additives, and silica membrane spin columns. All components are packaged in a sealed, barcoded box for traceability, supporting safe and organized sample processing from start to finish.

Trusted Technology and Reliable Results

Utilizing proven silica membrane-based RNA purification, the kit consistently delivers high-quality RNA. The carefully calibrated buffer system and components are optimized for reliable performance, meeting the requirements of applications such as real-time PCR and next-generation sequencing.

FAQ's of Qiagen RNeasy Mini QIAcube Kit (240):

Q: How does the Qiagen RNeasy Mini QIAcube Kit process samples for RNA extraction?

A: The kit uses a silica membrane-based technology to lyse cells and tissues, bind RNA, and perform thorough washing, all orchestrated in an automated workflow when used with the QIAcube or QIAcube Connect instruments. This allows for consistent, contamination-free RNA extraction.

Q: What types of samples can be processed with this kit and what is the maximum input amount?

A: You can process animal cells, animal tissues, yeast, and bacteria. The kit processes up to 1 x 10^7 cells or 30 mg of tissue per preparation, accommodating a variety of sample sources in genetic research.

Q: When should I use this kit compared to manual RNA extraction methods?

A: Opt for the RNeasy Mini QIAcube Kit when you require higher throughput, improved consistency, and minimized hands-on time. Its automation via the QIAcube platforms makes it especially suitable for busy labs handling multiple samples regularly.

Q: Where should I store the kit and its buffers for optimal shelf life?

A: Store the sealed kit at room temperature (15-25C). Certain buffer components may need refrigeration after opening, so check the label and the enclosed instructions or SDS for specific recommendations.

Q: What are the main benefits of using this kit for RNA purification?

A: The kit offers automated, high-yield, and high-purity RNA extraction, reducing the risk of sample degradation. Its ready-to-use components and barcoded traceability boost efficiency and data reliability for sensitive molecular biology applications.

Q: Can I use this kit manually, or is automation with QIAcube required?

A: While the kit is optimized for use with the QIAcube and QIAcube Connect instruments for automation, it can also be operated manually if necessary, following the detailed protocol provided.

Q: Is the kit suitable for downstream applications like gene expression and NGS?

A: Yes, the extracted RNA is of high integrity and purity, making it suitable for various sensitive downstream applications such as gene expression analysis, RT-PCR, real-time RT-PCR, microarray analysis, and next-generation sequencing (NGS).

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