QIAGEN Plasmid Plus 96 Miniprep Kit (4)

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Product Details:

Measurement Range For bacterial cultures up to 5 ml
Usage Type Laboratory/Research
Features High-purity plasmid DNA, 96-well format, rapid and convenient
Accuracy High recovery and purity
Function Purification of plasmid DNA from bacterial cultures
Shelf Life As stated by manufacturer, typically 1 year
Storage Instructions Store at room temperature, buffers at appropriate conditions as per protocol
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QIAGEN Plasmid Plus 96 Miniprep Kit (4) Price And Quantity

QIAGEN Plasmid Plus 96 Miniprep Kit (4) Product Specifications

Function Purification of plasmid DNA from bacterial cultures
Shelf Life As stated by manufacturer, typically 1 year
Accuracy High recovery and purity
Storage Instructions Store at room temperature, buffers at appropriate conditions as per protocol
Technology Silica-membrane based purification
Instruments Type 96-well Plate Format Kit
Use For rapid high-throughput purification of plasmid DNA
Power Source Not required (manual centrifugation or vacuum)
Operating Type Semi-automated/manual
Real-Time Operation No
Measurement Range For bacterial cultures up to 5 ml
Usage Type Laboratory/Research
Wall Mounted No
Equipment Type Plasmid DNA Purification Kit
Portable Yes
Features High-purity plasmid DNA, 96-well format, rapid and convenient
Color White/clear plates, assorted reagent colors (as per kit contents)
Condition New
Material High-quality plastic (96-well plates), reagents

Product Description

Brand Qiagen

Product Type PCR Kit

Pack Size 4 x 96 Wells

CAT No 16181

Application Molecular Reagent

For 4 x 96 plasmid minipreps: TurboFilter 96 Plates, Plasmid Plus 96 Plates, Buffers, Reagents, S-Blocks, and Elution Microtubes; requires use of QIAvac 96 and Elution Microtube Adapter (available from QIAGEN Technical Service), or a centrifugation system suitable for 96-well.

High-Throughput Convenience

Easily purify plasmid DNA from 96 bacterial samples in a single run with the 96-well plate design. This format accelerates lab workflows, making it ideal for research environments requiring efficiency and reliability. All steps are compatible with common laboratory centrifuges or vacuum manifolds, ensuring seamless operation.

Superior DNA Recovery and Purity

The silica-membrane-based extraction ensures that plasmid DNA is of the highest purity, free from contaminants and ready for critical molecular applications such as sequencing, cloning, or transfection. Expected yields reach up to 20 g per sample, optimizing the utility of each bacterial culture.

User-Friendly, Flexible Process

No electrical power is necessary-the kit operates using manual or semi-automated equipment. Clear instructions and fully supplied reagents streamline setup and daily use, accommodating a range of bacterial input volumes and offering reliable results for a multitude of laboratory research projects.

FAQ's of QIAGEN Plasmid Plus 96 Miniprep Kit (4):

Q: How does the QIAGEN Plasmid Plus 96 Miniprep Kit streamline plasmid DNA purification in the lab?

A: The kit allows for the simultaneous purification of plasmid DNA from up to 96 E. coli cultures using a silica-membrane 96-well plate. This high-throughput format saves time and increases productivity in workflows requiring large sample numbers.

Q: What type of samples can I process with this kit, and what yield should I expect?

A: You can use the kit with bacterial cultures, typically E. coli, in up to 5 ml volumes. The kit typically yields up to 20 g of highly pure plasmid DNA per preparation, supporting various downstream molecular biology applications.

Q: When should the purified DNA be used, and for which applications is it suitable?

A: The plasmid DNA should be used promptly after purification, or stored as recommended, for molecular cloning, sequencing, and transfection experiments. The DNA is of sufficient quality for these sensitive applications.

Q: Where can I use this kit, and what equipment is required for operation?

A: The kit can be used in any laboratory equipped with a centrifuge or vacuum manifold compatible with 96-well plates. It does not require an electrical power source, making it portable and flexible for research use.

Q: What is the general process involved in using the QIAGEN Plasmid Plus 96 Miniprep Kit?

A: The process involves lysing bacterial cultures, binding plasmid DNA to the silica membrane in the 96-well plate, washing contaminants away, and eluting purified DNA in 100-200 l elution buffer. Complete protocols and all required reagents are included.

Q: How should the kit and its components be stored for optimal performance?

A: Most kit components can be stored at room temperature, but certain buffers may require specific conditions as stated in the protocol. Proper storage ensures the kit retains a typical shelf life of about 1 year.

Q: What are the main benefits of using this kit over traditional miniprep methods?

A: This kit offers rapid, convenient purification in a high-throughput format, minimizing manual handling while maximizing reproducibility and DNA recovery. The result is consistently high-purity plasmid DNA suitable for demanding molecular biology applications.

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