Thermo Fisher Scientific Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit

Thermo Fisher Scientific Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit

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Product Details:

Features High signal strength, low background, reliable base calling, robust chemistry
Function DNA sequencing
Shelf Life 12 months (when stored as recommended)
Instruments Type Capillary electrophoresis genetic analyzers (compatible with Applied Biosystems platforms)
Measurement Range Read lengths up to 800-900 bases
Storage Instructions Store at -15C to -25C
Accuracy Up to 99% (with high-quality template DNA)
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Thermo Fisher Scientific Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit Price And Quantity

Reaction Volume Recommended: 10 l
Components BigDye Terminator Ready Reaction Mix v3.1, Sequencing Buffer, Control DNA, Primers
Lot Number/Expiry Date Provided on kit label
Application DNA sequencing, mutation analysis, genotyping, de novo sequencing, resequencing
Number of Reactions Varying pack sizes, e.g., 100 to 1,000 reactions
Safety Information For Research Use Only. Not for use in diagnostic procedures.
Compatible Instruments Applied Biosystems 3130, 3130xl, 3500, 3500xL, 3730, 3730xl Genetic Analyzers
Storage Temperature -20C
Kit Format Ready-to-use, contains all required components for sequencing reactions

Thermo Fisher Scientific Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit Product Specifications

Technology Dye terminator Sanger sequencing
Color Kit with color-coded reagents
Usage Type Laboratory, research, clinical research
Use DNA sequencing (Sanger); for genetic analysis and research
Material Reagents, enzymes, buffer solutions
Condition New
Weight Varies by kit size, typically less than 1 kg
Portable Yes
Equipment Type Sequencing Kit
Operating Type Manual, compatible with automated sequencers
Dimension (L*W*H) Not Applicable (kit form, consumables)
Real-Time Operation No
Accuracy Up to 99% (with high-quality template DNA)
Shelf Life 12 months (when stored as recommended)
Storage Instructions Store at -15C to -25C
Wall Mounted No
Function DNA sequencing
Measurement Range Read lengths up to 800-900 bases
Instruments Type Capillary electrophoresis genetic analyzers (compatible with Applied Biosystems platforms)
Features High signal strength, low background, reliable base calling, robust chemistry

Reaction Volume Recommended: 10 l
Components BigDye Terminator Ready Reaction Mix v3.1, Sequencing Buffer, Control DNA, Primers
Lot Number/Expiry Date Provided on kit label
Application DNA sequencing, mutation analysis, genotyping, de novo sequencing, resequencing
Number of Reactions Varying pack sizes, e.g., 100 to 1,000 reactions
Safety Information For Research Use Only. Not for use in diagnostic procedures.
Compatible Instruments Applied Biosystems 3130, 3130xl, 3500, 3500xL, 3730, 3730xl Genetic Analyzers
Storage Temperature -20C
Kit Format Ready-to-use, contains all required components for sequencing reactions

Product Description

Brand	Thermo Fisher Scientific
Product Type	Sequencing Kit
Pack Size	25,000 Reactions
CAT No	4337458
Application	Molecular Biology

The BigDye Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

Exceptional Sequencing Performance

The BigDye Terminator v3.1 Cycle Sequencing Kit leverages advanced dye terminator Sanger technology, delivering high signal intensity and accuracy up to 99% when used with quality template DNA. The optimized reagent mix ensures robust and consistent results for both standard and challenging templates, supporting read lengths of up to 900 bases.

Ready-to-Use Format Simplifies Workflow

With all components supplied in color-coded, ready-to-use form, this kit streamlines the sequencing setup process. Researchers benefit from reduced hands-on time and minimal reagent preparation, increasing laboratory efficiency and data turnaround for diverse sequencing projects.

Wide Instrument Compatibility

The kit is fully compatible with major Applied Biosystems capillary electrophoresis analyzers, including 3130/3130xl, 3500/3500xL, and 3730/3730xl series. Its versatile format supports a range of applications, from basic research to clinical research use, making it a reliable choice for laboratories across India.

FAQ's of Thermo Fisher Scientific Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit:

Q: How do I store the BigDye Terminator v3.1 Cycle Sequencing Kit for optimal stability?

A: The kit should be stored at -15C to -25C (typically at -20C). Following these guidelines helps maintain reagent integrity and ensures optimal performance throughout its 12-month shelf life.

Q: What applications are supported by this sequencing kit?

A: This kit supports a variety of DNA sequencing applications, including mutation analysis, genotyping, de novo sequencing, and re-sequencing, making it suitable for genetic analysis and a wide range of research purposes.

Q: Which instruments are compatible with the BigDye Terminator v3.1 Kit?

A: The kit is compatible with Applied Biosystems capillary electrophoresis genetic analyzers, specifically the 3130, 3130xl, 3500, 3500xL, 3730, and 3730xl platforms.

Q: When should I use the control DNA and primers provided in the kit?

A: The control DNA and primers are included to validate the sequencing process and reagent performance. Use them during initial runs or when troubleshooting for consistent, reliable results.

Q: How many reactions can I perform with each kit?

A: Pack sizes vary, allowing users to choose from kits supporting 100 up to 1,000 reactions, depending on laboratory throughput needs.

Q: What is the recommended reaction volume for optimal sequencing results?

A: A 10 l reaction volume is recommended for most sequencing applications to ensure high-quality results while achieving maximum reagent efficiency.

Q: What benefits does the BigDye Terminator v3.1 Kit offer for DNA sequencing?

A: Users benefit from high signal strength, low background, robust base calling, and up to 900 base read lengths, ensuring precise and reliable sequencing data for research and analysis.

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