Product Description
Brand Qiagen
Product Type dPCR Kit
Pack Size 200 x 12 L
CAT No 250205
Application Molecular Reagent
dPCR Copy Number Assays enable specific, accurate, reproducible and easy-to-interpret copy number change analysis for an individual gene or region of interest. Assays for more than 200 targets have been dPCR wet-lab tested. All other in silico designs have been bench-verified and are ready to use in NGS follow-up studies, specific target screening and other related studies.
High-Performance Digital PCR AssaysThe Qiagen dPCR Copy Number Assay is engineered for exceptional accuracy and sensitivity in detecting DNA copy number variations across diverse biological samples. Its pre-mixed, ready-to-use format accelerates workflow and ensures consistent results, even when working with low input DNA quantities. Compatibility with QIAGEN dPCR systems, including QIAcuity, enhances flexibility for laboratories handling varied and high-throughput research projects.
Ready for Research DemandsDesigned to suit laboratories in research and diagnostics, this assay seamlessly quantifies copy number variations in human, animal, plant, and microbial DNA. The kit format supports medium to high throughput demands, while proprietary consumables deliver reliable performance. With a shelf life of 12 months and straightforward storage requirements, laboratories can count on uninterrupted project continuity.
FAQ's of Qiagen dPCR Copy Number Assay (200):
Q: How is the Qiagen dPCR Copy Number Assay used to quantify DNA copy number variations?
A: This assay employs digital PCR (dPCR) technology, partitioning DNA samples to allow precise detection and quantification of copy number variations. Users simply follow the protocol using the ready-to-use mix on compatible QIAGEN dPCR platforms, such as QIAcuity, for single or multiplex analysis.
Q: What types of samples can be analyzed with this assay?
A: The assay is validated for detecting copy number variations in DNA derived from human, animal, plant, and microbial sources, offering broad utility across research disciplines.
Q: When should I choose this assay for my applications?
A: This assay is ideal when your project demands accurate, sensitive quantification of DNA copy number variations, especially when working with limited amounts of input DNA or diverse sample types. It performs exceptionally well in medium to high-throughput laboratory environments.
Q: Where can the Qiagen dPCR Copy Number Assay be implemented?
A: It is designed for use in research and laboratory settings on digital PCR systems compatible with QIAGEN's assays, including the QIAcuity platform. It is not intended for point-of-care or portable usage.
Q: What is the process for running the assay?
A: Preparation involves adding sample DNA to the ready-to-use reagent mix and loading it onto the dPCR instrument according to the provided instructions. The instrument automates partitioning and detection, after which results are analyzed using compatible software.
Q: What are the storage requirements and shelf life for this assay kit?
A: The assay kit should be stored at -20C to maintain reagent integrity. It has a shelf life of 12 months from the date of manufacturing.
Q: What benefits does the Qiagen dPCR Copy Number Assay offer over traditional methods?
A: Compared to conventional PCR techniques, the digital PCR format of this assay provides higher precision, sensitivity, and quantitative accuracy, supporting detection across a wide dynamic range and enabling reliable analysis of copy number variations even with minimal DNA input.