Qiagen QIAamp ccfDNA/RNA Kit (50)

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Product Details:

Features High-sensitivity processing; efficient recovery of low-concentration nucleic acids
Function Extraction and purification of ccfDNA and ccfRNA
Accuracy High, suitable for downstream applications
Usage Type Laboratory/Research
Storage Instructions Store at room temperature (1525C)
Measurement Range Suitable for sample volumes up to 5 ml plasma or serum
Shelf Life At least 12 months from manufacture date
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Qiagen QIAamp ccfDNA/RNA Kit (50) Price And Quantity

Qiagen QIAamp ccfDNA/RNA Kit (50) Product Specifications

Material Kit reagents, spin columns, buffers
Instruments Type Sample preparation kit
Function Extraction and purification of ccfDNA and ccfRNA
Condition New
Color White/Blue (kit packaging)
Portable Yes
Wall Mounted No
Equipment Type cfDNA/RNA Isolation Kit
Operating Type Manual
Weight Approx. 1 kg (packed kit)
Dimension (L*W*H) N/A (Kit box dimensions vary)
Technology Silica-membrane technology
Use Efficient extraction and purification of circulating cell-free DNA and RNA from plasma or serum samples
Shelf Life At least 12 months from manufacture date
Measurement Range Suitable for sample volumes up to 5 ml plasma or serum
Storage Instructions Store at room temperature (1525C)
Features High-sensitivity processing; efficient recovery of low-concentration nucleic acids
Usage Type Laboratory/Research
Accuracy High, suitable for downstream applications
Real-Time Operation Yes (Compatible with real-time PCR workflows)

Product Description

Brand Qiagen

Product Type PCR Kits

Pack Size 50T

CAT No 55184

Application Molecular Reagent

The QIAamp ccfDNA/RNA Kit enables co-purification of cell-free DNA and RNA, including vesicular and non-vesicular nucleic acids. Starting from 14 ml plasma, the protocol uses midi spin columns, removing the need for a vacuum step. For nucleic acid elution, MinElute spin columns allow for small elution volumes of 14 l. The kit ensures high yields of cell-free DNA and total RNA including miRNA from plasma and serum samples.

Efficient Processing for Liquid Biopsy Applications

This kit enables rapid and consistent isolation of high-quality cell-free DNA and RNA, catering to the needs of advanced clinical and research investigations such as prenatal screening, cancer diagnostics, and infectious disease monitoring. Its high sensitivity ensures excellent recovery even from limited or dilute samples, supporting reliable downstream analysis.

User-Friendly, Complete Solution

Providing all necessary reagents and consumables, including spin columns and buffers, the Qiagen QIAamp ccfDNA/RNA Kit is designed for manual, hands-on operation. A detailed protocol booklet guides users from start to finish, making it suitable for both seasoned researchers and new laboratory personnel in molecular biology or clinical research settings.

FAQ's of Qiagen QIAamp ccfDNA/RNA Kit (50):

Q: How does the QIAamp ccfDNA/RNA Kit isolate circulating nucleic acids from samples?

A: This kit uses silica-membrane technology, which binds cell-free DNA and RNA from plasma or serum. Following lysis and proteolytic treatment, nucleic acids are captured on the membrane, washed, and then eluted in RNase-free water for downstream applications.

Q: What types of samples can be processed with this kit?

A: The kit is optimized for plasma and serum samples. For each prep, up to 5 ml of plasma or serum can be used, ensuring compatibility with most liquid biopsy requirements in research and clinical settings.

Q: When should I use this kit in my research or diagnostic workflow?

A: This kit is ideal when you need to isolate ccfDNA or ccfRNA for sensitive analyses, such as NIPT, oncology liquid biopsies, infectious disease detection, or biomarker discovery. It is especially valuable when sample volumes are limited or nucleic acid concentrations are low.

Q: Where should I store the kit and how long is it usable?

A: Store the kit at room temperature (15-25C). It is stable for at least 12 months from the manufacture date; the specific lot number and expiry date can be found on each kit.

Q: What equipment is required for using this isolation kit?

A: Standard laboratory centrifuges compatible with spin columns are required. No specialized instrumentation is needed, making the kit suitable for most molecular biology and clinical laboratories.

Q: What are the benefits of using this kit compared to traditional methods?

A: This kit offers high sensitivity for low-concentration nucleic acids, a streamlined protocol, and consistent, reproducible results. Its compatibility with real-time PCR enables efficient transition to downstream analyses without additional cleanup steps.

Q: How long does the entire isolation process take and how many samples can be processed simultaneously?

A: Processing 24 preps typically takes about 90 minutes. The kit provides sufficient reagents for 50 preparations, allowing for flexible batch processing as needed in your workflow.

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