Product Description
| Brand |
Thermo Fisher Scientific |
| Product Type |
PCR Kit |
| Pack Size |
5000 Reactions |
| CAT No |
12532024 |
| Application |
Molecular Biology |
Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Designed for high-specificity, high-fidelity DNA amplification, Platinum PCR SuperMix reduces reaction set-up time and only requires the addition of template and primer.
High-Fidelity PCR PerformanceThis SuperMix is engineered for demanding applications that require highly accurate DNA amplification. Its unique blend of Platinum Taq DNA Polymerase High Fidelity and proprietary proofreading enzymes significantly reduces error rates, making it ideal for projects like cloning, site-directed mutagenesis, or sequencing where fidelity is paramount.
Simplified Workflow and Ready-to-Use ConvenienceBy offering a complete, pre-mixed 2X solution, the Platinum PCR SuperMix eliminates the need to prepare separate reaction components. This approach not only streamlines experimental setup but also limits pipetting error and ensures highly consistent, reproducible results across your PCR assays.
Storage, Stability, and ShippingSupplied as a colorless solution, the SuperMix is shipped on dry ice to preserve enzyme activity and must be stored at -20C. When stored properly, the mixture maintains effectiveness for up to 12 months. This stability supports both routine laboratory work and larger project planning without concern for product degradation.
FAQ's of Thermo Fisher Scientific Invitrogen Platinum PCR SuperMix High Fidelity:
Q: How should Platinum PCR SuperMix High Fidelity be prepared and used in PCR reactions?
A: It comes as a 2X ready-to-use solution; combine equal volumes of the SuperMix and your primers/template DNA to achieve a final reaction mix with recommended concentrations. No additional buffer or components are needed, which greatly reduces preparation time and pipetting variation.
Q: What applications are best suited for this PCR SuperMix?
A: This reagent is specialized for applications demanding high-fidelity DNA amplification, such as cloning, mutagenesis, and sequencing, where accuracy and reduced mutation rates are critical for downstream work.
Q: When should I use this SuperMix instead of standard Taq DNA polymerase?
A: Choose Platinum PCR SuperMix High Fidelity when experiments require precise DNA replication with minimal errors. Its engineered enzyme blend yields approximately sixfold greater fidelity than conventional Taq, making it preferable for applications sensitive to mutations.
Q: Where should the product be stored to maintain its shelf life?
A: The SuperMix must be stored at -20C to preserve enzyme activity and ensure its 12-month shelf life. Avoid repeated freeze-thaw cycles to maintain optimal performance.
Q: What are the benefits of using this PCR SuperMix over assembling individual PCR components?
A: As a fully formulated mix, it minimizes pipetting steps, reducing the risk of human error and variability. It delivers consistent, reproducible results, saves time, and improves workflow efficiency-ideal for laboratories handling many PCR samples.
Q: How does the inhibitor resistance of this SuperMix compare to other PCR mixes?
A: This product offers moderate resistance to common PCR inhibitors, allowing reliable performance with standard templates but may require additional optimization if working with highly impure samples.
Q: Can the SuperMix be used for long-range PCR, and what is its maximum amplification range?
A: Yes, the SuperMix is capable of amplifying targets up to 15 kb, making it suitable for many long-range PCR applications while ensuring high fidelity.