Qiagen QuantiNova LNA PCR Assay (750)

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Product Details:

Function Quantitative PCR (qPCR) detection
Shelf Life 12 months (when stored properly)
Storage Instructions Store at -20C
Features High specificity and sensitivity for target detection, LNA-modified primers and probes
Usage Type Research Use Only (RUO)
Accuracy High accuracy, dependent on protocol adherence
Instruments Type Compatible with standard real-time PCR cyclers
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Qiagen QuantiNova LNA PCR Assay (750) Price And Quantity

Qiagen QuantiNova LNA PCR Assay (750) Product Specifications

Storage Instructions Store at -20C
Features High specificity and sensitivity for target detection, LNA-modified primers and probes
Power Source Not applicable (consumable; used with PCR instruments)
Use Quantitative detection of nucleic acids in research applications
Function Quantitative PCR (qPCR) detection
Condition New
Color Kit components: typically clear or colorless
Shelf Life 12 months (when stored properly)
Usage Type Research Use Only (RUO)
Accuracy High accuracy, dependent on protocol adherence
Operating Type Automated PCR based
Measurement Range Wide dynamic range suitable for low and high copy number targets
Instruments Type Compatible with standard real-time PCR cyclers
Real-Time Operation Yes
Wall Mounted No
Technology Locked Nucleic Acid (LNA) enhanced real-time PCR
Equipment Type PCR Assay Kit

Product Description

Brand Qiagen

Product Type PCR Kit

Pack Size 750T

CAT No 249992

Application Molecular Reagent

QuantiNova LNA PCR Assays are setting new standards in SYBR Green-based qPCR gene expression analysis. Choose from the broadest selection of predesigned assays for accurate and sensitive detection of any human, mouse or rat mRNA or lncRNA from general transcript detection to differentiation of specific transcript isoforms.LNA enhancementallows us to design shorter primers, which are more flexible to position on the target. As a result, we can optimize our designs for specific transcript detection or target differentiation.

Unmatched Detection Sensitivity and Specificity

With its LNA-modified primers and probes, the QuantiNova LNA PCR Assay delivers high specificity and sensitivity, enabling detection and quantification of even low-abundance targets. This makes the kit highly reliable for challenging samples where accuracy is paramount.

Ready-to-Use Convenience

Supplied in lyophilized or liquid format, the kit components are optimized for ease of use and compatibility with standard real-time PCR instruments. The master mix and controls streamline the experimental setup, minimizing variability and saving valuable laboratory time.

FAQ's of Qiagen QuantiNova LNA PCR Assay (750):

Q: How should the QuantiNova LNA PCR Assay (750) be stored to maintain optimal performance?

A: Store the kit at -20C, ensuring it is protected from light and moisture. Adhering to these storage conditions will help preserve reagent integrity and the overall effectiveness of the assay for up to 12 months.

Q: What sample types are compatible with the QuantiNova LNA PCR Assay (750)?

A: The kit supports a wide range of sample types, including cDNA, RNA, or DNA obtained from various biological specimens, ensuring versatility for numerous research applications.

Q: When is it appropriate to use this kit in my research workflow?

A: This assay is ideal when quantitative detection of nucleic acids is required, such as during gene expression profiling, SNP genotyping, or pathogen identification in research environments.

Q: Where can I use the QuantiNova LNA PCR Assay (750)?

A: The assay is designed for use in molecular biology and biomedical research laboratories equipped with standard real-time PCR cyclers. It is intended for research use only (RUO) and not for diagnostic or clinical procedures.

Q: What benefits do LNA-modified primers and probes provide in this assay?

A: LNA modifications enhance the melting temperature and stability of the oligonucleotides, resulting in improved specificity, sensitivity, and overall accuracy in target detection during qPCR experiments.

Q: How many assays can be performed with one kit and what applications does it support?

A: Each kit supplies enough reagents for 750 reactions, supporting diverse applications such as quantitative gene expression analysis, SNP detection, and pathogen identification from various nucleic acid sources.

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