Product Description
Brand Qiagen
Product Type PCR and RT-PCR Kit
Pack Size 4 x 100 l, 4 x 250 l
CAT No 201912, 201913
Application Molecular Reagent
dNTP Set, PCR Grade is a complete set of individual, highly pure dNTPs. Each individual 100 mM dNTP is supplied in water and can be diluted and mixed with any other dNTP to the desired concentration. dNTP Mix, PCR Grade contains premixed dATP, dCTP, dGTP, and dTTP in water (pH 7.5), each at a concentration of 10 mM. Highly pure dNTPs are important for successful PCR, as the presence of contaminating impurities in PCR can result in a decrease in amplification sensitivity and product yield.
High Purity dNTPs for Reliable ResultsEach nucleotide in the Qiagen dNTP Set is of molecular biology grade, surpassing 99% purity (HPLC tested). Such quality ensures precise, reproducible results in PCR, RT-PCR, cDNA synthesis, DNA labeling, and sequencing. The aqueous solution formulation guarantees user-friendly preparation and integration in molecular workflows.
Convenient Format and Secure PackagingThis dNTP set arrives in four securely sealed, colorless screw-cap tubes, each containing 100 l. The ready-to-use configuration simplifies experimental setup, prevents contamination, and supports efficient workflow in research or clinical laboratories. The set's portability and ease of storage at -20C enhance user experience.
FAQ's of Qiagen dNTP Set, PCR Grade:
Q: How should the Qiagen dNTP Set, PCR Grade, be stored?
A: For maximum stability and to maintain purity, the dNTP set should be stored at -20C. This ensures the reagents remain free from degradation and suitable for PCR applications throughout the 12-month shelf life.
Q: What applications is this dNTP set suitable for?
A: The Qiagen dNTP Set, PCR Grade, is designed for PCR, quantitative PCR, cDNA synthesis, DNA labeling, sequencing, and related molecular biology applications, where high-purity reagents are essential.
Q: When should I use this dNTP set in my experiments?
A: This set is ideal for all standard and real-time PCR protocols, as well as molecular procedures requiring precise DNA synthesis, such as RT-PCR and sequencing. It should be used at recommended concentrations for optimal results.
Q: Where can this dNTP set be utilized?
A: This reagent set is intended for use in molecular biology and research laboratories. It is widely suitable for academic, clinical, and industrial labs across India and internationally.
Q: What is the process for using the Qiagen dNTP Set in PCR?
A: Simply thaw the dNTPs on ice, briefly vortex, and add the required volume to your PCR master mix. Ensure uniform mixing and keep the reagents cold to preserve activity until use.
Q: Why is high purity important in dNTPs for PCR?
A: High-purity dNTPs, such as those in this Qiagen set (>99% by HPLC), minimize the risk of unwanted side reactions and ensure high-fidelity DNA amplification, supporting reliable reproducibility and accurate molecular results.