Thermo Fisher Scientific Applied Biosystems TaqMan Fast Virus 1-Step Master Mix for qPCR

Thermo Fisher Scientific Applied Biosystems TaqMan Fast Virus 1-Step Master Mix for qPCR

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Product Details:

Instruments Type Real-Time PCR Systems
Storage Instructions Store at -20C
Usage Type Laboratory research and diagnostics
Accuracy High specificity and sensitivity
Features Robust performance, compatible with multiplexing, optimized for fast cycling
Shelf Life 12 months
Measurement Range Wide dynamic range, detects low viral titers
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Thermo Fisher Scientific Applied Biosystems TaqMan Fast Virus 1-Step Master Mix for qPCR Price And Quantity

Format 2X master mix
Application RNA viral detection, gene expression analysis, pathogen quantitation
Compatible Instruments All major real-time PCR instruments
Reaction Time Fast cycling protocols, ~40 minutes
Catalog Number 4444432 (common), varies by size and format
Enzyme Components Contains thermostable reverse transcriptase and hot-start DNA polymerase
Sample Volume Compatibility Compatible with low sample input
RNase Inhibitor Included in formulation
Package Size 1 mL, 5 mL, and custom sizes available

Thermo Fisher Scientific Applied Biosystems TaqMan Fast Virus 1-Step Master Mix for qPCR Product Specifications

Shelf Life 12 months
Technology TaqMan Probe-Based Real-Time qPCR
Measurement Range Wide dynamic range, detects low viral titers
Features Robust performance, compatible with multiplexing, optimized for fast cycling
Portable Yes
Accuracy High specificity and sensitivity
Condition New
Instruments Type Real-Time PCR Systems
Storage Instructions Store at -20C
Usage Type Laboratory research and diagnostics
Function Enables cDNA synthesis and qPCR amplification in a single tube
Operating Type Automated
Wall Mounted No
Real-Time Operation Yes
Use For fast, one-step real-time RT-qPCR detection of RNA viruses
Color Clear
Equipment Type qPCR One-Step Master Mix
Material Proprietary enzyme blend, buffer system, and stabilizers

Format 2X master mix
Application RNA viral detection, gene expression analysis, pathogen quantitation
Compatible Instruments All major real-time PCR instruments
Reaction Time Fast cycling protocols, ~40 minutes
Catalog Number 4444432 (common), varies by size and format
Enzyme Components Contains thermostable reverse transcriptase and hot-start DNA polymerase
Sample Volume Compatibility Compatible with low sample input
RNase Inhibitor Included in formulation
Package Size 1 mL, 5 mL, and custom sizes available

Product Description

Brand	Thermo Fisher Scientific
Product Type	Master Mix
Pack Size	1000 Reactions
CAT No	4444434
Application	Molecular Biology

TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR even in the presence of challenging PCR inhibitors. The 4X formulation provides enhanced detection of both RNA and DNA viruses and is ideal for multiplex gene expression studies, even with low target input. The TaqMan Fast Virus 1-Step Master Mix provides a single-tube format for uniform handling and processing, while delivering qPCR cycling in less than 30 minutes, helpful when working in high throughput workflows.

Accelerated Real-Time RT-qPCR Workflow

Experience high-throughput detection of RNA viruses and gene expression analysis with the TaqMan Fast Virus 1-Step Master Mix. Its advanced enzyme blend enables both reverse transcription and PCR amplification in a single tube, reducing hands-on time and the risk of contamination. Complete your workflow in approximately 40 minutes, streamlining diagnostic and research applications.

Broad Compatibility and High Sensitivity

This master mix is formulated for exceptional performance across all major real-time PCR systems. It works efficiently with low input samples and supports multiplexing, enabling simultaneous detection of multiple targets. The robust proprietary buffer system ensures specificity, sensitivity, and a wide dynamic detection range-including low viral titers.

Reliable Storage and Easy Handling

TaqMan Fast Virus 1-Step Master Mix has a shelf life of 12 months when stored at -20C. The ready-to-use 2X formulation simplifies reaction setup and is available in sizes suited for varied laboratory needs. Its robust formulation and included RNase inhibitor ensure the reliability of your results, run after run.

FAQ's of Thermo Fisher Scientific Applied Biosystems TaqMan Fast Virus 1-Step Master Mix for qPCR:

Q: How does the TaqMan Fast Virus 1-Step Master Mix simplify the qPCR workflow?

A: This master mix enables both cDNA synthesis from RNA and real-time PCR amplification in a single reaction tube. This streamlined, one-step approach reduces sample handling, shortens reaction time to around 40 minutes, and minimizes the risk of contamination.

Q: What types of applications is this master mix suitable for?

A: It is specifically designed for fast detection of RNA viruses, gene expression analysis, and quantitative assessment of pathogen load in research and diagnostic laboratories.

Q: When should I use this product instead of a traditional multi-step RT-qPCR protocol?

A: Use the TaqMan Fast Virus 1-Step Master Mix when you require rapid results, minimal sample processing, and reduced opportunity for sample loss or contamination-particularly in high-throughput or time-sensitive laboratory settings.

Q: Where can I use this master mix in terms of instrumentation?

A: The formulation is compatible with all major real-time PCR instruments, making it suitable for use in a wide range of laboratories and research facilities.

Q: What is the recommended storage condition for this master mix, and how long is its shelf life?

A: Store the product at -20C to maintain performance and stability. Under proper storage, the master mix retains full activity for up to 12 months.

Q: What benefits does the included RNase inhibitor provide?

A: The RNase inhibitor embedded in the formulation protects RNA templates from degradation, enhancing the accuracy and reliability of your RT-qPCR results-especially when handling low input samples.

Q: Is this master mix effective for multiplexing applications?

A: Yes, the robust buffer system and enzyme blend are optimized to support multiplex reactions, allowing simultaneous detection of multiple targets within a single run.

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