Product Description
Brand Qiagen
Product Type PCR Kit
Pack Size 3 x 1 ml
CAT No 250111
Application Molecular Reagent
The QIAcuity EG PCR Kit contains a 3x concentrated, ready-to-use Master Mix optimized for microfluidic use in the QIAcuity Nanoplates. The kit improves the specificity and efficiency of dye-based digital PCR to provide accurate quantitation analysis. An intercalating dye EvaGreen binds to double-stranded DNA and enhances the quantitative accuracy of gDNA or cDNA measurements on the QIAcuity dPCR instruments.
Advanced Digital PCR PerformanceEngineered for precision, the QIAcuity EG PCR Kit delivers powerful endpoint PCR performance in QIAcuity systems. Its high-sensitivity, EvaGreen dye-based chemistry and proprietary buffer blend minimize PCR inhibition and maximize accuracy, supporting trusted digital quantification for a wide dynamic range.
Streamlined Laboratory EfficiencyBenefit from high throughput sample processing and compatibility with automated workflows. The included barcode simplifies inventory management, making this kit ideal for research labs and high-volume testing environments. Its clear, colorless solution allows easy handling and tracking during experimental runs.
FAQ's of Qiagen QIAcuity EG PCR Kit (1 ml):
Q: How should I store the QIAcuity EG PCR Kit to maintain its shelf life?
A: The kit should be stored at temperatures between -15C and -25C as recommended. It is shipped on dry ice and must remain frozen to ensure all reagents maintain their stability and activity for up to one year.
Q: What equipment is this PCR kit compatible with?
A: This kit is specifically designed for use with Qiagen's QIAcuity digital PCR systems. Its chemistry and format are optimized for end-point EvaGreen-based detection exclusively on these platforms.
Q: When should I use this kit in my workflow?
A: The QIAcuity EG PCR Kit is ideal for digital PCR applications requiring high accuracy and reproducibility, such as quantification of nucleic acids, rare mutation detection, or copy number variation studies in research or laboratory settings.
Q: Where is this kit primarily used?
A: Designed for use in research laboratories, molecular diagnostics facilities, and core labs that deploy QIAcuity digital PCR systems, the kit supports high-throughput workflows and automated platforms.
Q: What is the process of using this kit in a digital PCR workflow?
A: To use, combine sample DNA with the provided mastermix and perform digital PCR according to QIAcuity system protocols. The EvaGreen dye detects amplified products at end-point, enabling robust quantification across a wide dynamic range.
Q: What are the benefits of using the QIAcuity EG PCR Kit?
A: Users benefit from high sensitivity, robust performance, and reduced PCR inhibition. Barcoding enables efficient tracking, and batch-testing ensures excellent reproducibility, making it ideal for consistent, high-throughput applications.