Thermo Scientific Maxima SYBR Green/ROX qPCR Master Mix (2X)

Thermo Scientific Maxima SYBR Green/ROX qPCR Master Mix (2X)

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Product Details:

Accuracy High (optimized formulation enables precise quantification)
Shelf Life 12 months
Instruments Type Compatible with qPCR instruments that support ROX passive reference dye
Storage Instructions Store between -20C and -25C; avoid repeated freeze/thaw cycles
Measurement Range Wide dynamic range for DNA targets (typically 10 fg to 100 ng)
Function Enables detection and quantification of nucleic acids in real-time PCR
Features High sensitivity, specific amplification, optimized for ROX normalization, ready-to-use 2X formulation
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Thermo Scientific Maxima SYBR Green/ROX qPCR Master Mix (2X) Price And Quantity

Thermo Scientific Maxima SYBR Green/ROX qPCR Master Mix (2X) Product Specifications

Dimension (L*W*H) Not specified (supplied as liquid reagent in vials/tubes)
Condition New
Use Quantitative PCR, gene expression analysis, DNA quantification
Material Nucleic acid reagents (buffered solution containing SYBR Green, ROX dye, dNTPs, Taq DNA polymerase, MgCl2)
Real-Time Operation Yes
Color Green (due to SYBR Green dye)
Accuracy High (optimized formulation enables precise quantification)
Portable Yes (small vial or tube format for easy handling)
Function Enables detection and quantification of nucleic acids in real-time PCR
Features High sensitivity, specific amplification, optimized for ROX normalization, ready-to-use 2X formulation
Storage Instructions Store between -20C and -25C; avoid repeated freeze/thaw cycles
Measurement Range Wide dynamic range for DNA targets (typically 10 fg to 100 ng)
Wall Mounted No
Operating Type Manual (used in automated and manual qPCR workflows)
Equipment Type qPCR Master Mix
Technology Real-time quantitative PCR (qPCR)
Shelf Life 12 months
Instruments Type Compatible with qPCR instruments that support ROX passive reference dye
Power Source Not required (used in conjunction with qPCR instruments)
Usage Type Laboratory

Stability Stable for up to 12 months under recommended storage conditions
dNTP Concentration Optimized for sensitive and robust amplification
Product Name Maxima SYBR Green/ROX qPCR Master Mix (2X)
Lot Number As per pack label
Expiration Date See packaging
Detection Dye SYBR Green
Pack Size Available in multiple vial sizes (e.g., 1 mL, 5 mL, 10 mL)
Polymerase Type Maxima Hot Start Taq DNA Polymerase
MgCl2 Concentration Pre-optimized
Application Gene expression quantification, DNA template detection
Reference Dye ROX
Format 2X concentrated, ready-to-use master mix

Product Description

Brand	Thermo Fisher Scientific
Product Type	Master Mix
Pack Size	4,000 Reactions
CAT No	K0223
Application	Molecular Biology

Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Ready-To-Use for Reliable Quantification

Maxima SYBR Green/ROX qPCR Master Mix (2X) streamlines qPCR setup by providing a pre-formulated blend of essential components. With its high specificity and optimized composition, this master mix is suitable for a variety of gene expression and DNA quantification experiments in research or diagnostic settings.

Optimized Formulation for Consistency

Every batch comes pre-optimized with dNTPs and MgCl2, ensuring consistent results and reducing variability between runs. The included ROX dye supports normalization, especially with qPCR instruments that require a passive reference, while SYBR Green enables sensitive detection of double-stranded DNA.

Convenient and Stable Packaging

Available in multiple pack sizes to match different laboratory needs, the mix is supplied in liquid form for easy handling and portability. The shelf life is 12 months when stored at -20C to -25C, making it a practical choice for laboratories seeking stable and long-lasting reagents.

FAQ's of Thermo Scientific Maxima SYBR Green/ROX qPCR Master Mix (2X):

Q: How do I use the Maxima SYBR Green/ROX qPCR Master Mix (2X) in my qPCR experiments?

A: Simply combine the 2X master mix with your primers, template DNA, and nuclease-free water according to your protocol. The reagent is ready-to-use and compatible with both manual and automated pipetting, streamlining your gene expression or DNA quantification workflows.

Q: What are the benefits of using this master mix for quantitative PCR?

A: It offers high sensitivity and specificity due to Maxima Hot Start Taq DNA Polymerase, optimized dNTPs, and MgCl2. The addition of SYBR Green provides robust detection, and ROX allows for improved normalization, resulting in precise and reproducible qPCR results.

Q: When should I use the various pack sizes available for this qPCR master mix?

A: Choose the appropriate vial size based on your throughput and frequency of use. Smaller labs or pilot studies may opt for 1 mL vials, whereas high-volume users might prefer larger packs like 5 mL or 10 mL to ensure efficiency and reduce storage demands.

Q: Where should I store the Maxima SYBR Green/ROX qPCR Master Mix (2X)?

A: Store the reagent between -20C and -25C as indicated on the packaging. Strictly avoid repeated freeze/thaw cycles to preserve the activity of the enzyme and stability of all components throughout its 12-month shelf life.

Q: What type of qPCR instruments are compatible with this master mix?

A: This master mix is designed for use with real-time PCR instruments that can accommodate ROX as a passive reference dye. Always check your instrument's requirements to enable optimal performance with this product.

Q: How does the inclusion of ROX dye benefit my qPCR experiments?

A: ROX dye serves as a passive reference, helping to normalize for signal fluctuations and pipetting differences during real-time PCR. This enhances the accuracy and reliability of quantification, particularly in instruments that require such normalization.

Q: What is the functional measurement range for DNA detection with this master mix?

A: Maxima SYBR Green/ROX qPCR Master Mix supports a wide dynamic range, reliably quantifying DNA targets typically within 10 fg to 100 ng. This makes it suitable for diverse experimental applications requiring sensitive and accurate DNA measurement.

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