Qiagen QIAfilter Plasmid Midi Kit (100)

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Product Details:

Instruments Type Molecular Biology Consumable
Usage Type Laboratory, Research
Shelf Life 12 months
Function Isolation and purification of plasmid DNA
Accuracy High DNA yield and purity
Storage Instructions Store at room temperature (1525C), avoid moisture and direct sunlight
Measurement Range Purifies up to 100 g of plasmid DNA per preparation
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Qiagen QIAfilter Plasmid Midi Kit (100) Price And Quantity

Qiagen QIAfilter Plasmid Midi Kit (100) Product Specifications

Real-Time Operation No
Material Polypropylene, High-grade plastic and filter membrane
Features Integrated QIAfilter cartridge, rapid filtration, high purity yield, easy-to-use protocol
Storage Instructions Store at room temperature (1525C), avoid moisture and direct sunlight
Measurement Range Purifies up to 100 g of plasmid DNA per preparation
Technology Alkaline lysis with anion-exchange chromatography filtration
Use For rapid purification of plasmid DNA from bacterial cultures
Weight Approx. 3.2 kg per kit
Equipment Type Plasmid Purification Kit
Accuracy High DNA yield and purity
Portable Yes
Dimension (L*W*H) Kit box approx. 34 x 29 x 15 cm
Function Isolation and purification of plasmid DNA
Usage Type Laboratory, Research
Shelf Life 12 months
Noise Level Silent Operation
Wall Mounted No
Instruments Type Molecular Biology Consumable
Color White/Blue
Operating Type Manual
Power Source Not required (manual kit)
Condition New

Product Description

Brand Qiagen

Product Type QIAfilter

Pack Size 100T

CAT No 12245

Application Molecular Reagent

QIAfilter Plasmid Kits provide anion-exchange-based plasmid purification with bacterial lysate clearing by filtration. Purified DNA is equivalent to that obtained by 2 x CsCl gradient centrifugation and is suitable for transfection-grade applications.

Efficient Plasmid DNA Purification

The QIAfilter Plasmid Midi Kit offers a streamlined protocol using a combination of alkaline lysis and anion-exchange filtration. It is designed for isolating high-yield plasmid DNA from E. coli with minimal contaminants. Researchers benefit from rapid processing and consistently reliable results, making it ideal for demanding applications such as cloning, transfection, and sequencing.

User-Friendly Workflow and Components

Featuring an integrated QIAfilter cartridge and midi spin columns, this kit simplifies and accelerates sample processing. All essential reagents, including RNase A and multiple buffer types, are included for straightforward assembly. The clear protocol and minimal equipment requirements make this kit suitable for both novice and experienced laboratory personnel.

FAQs of Qiagen QIAfilter Plasmid Midi Kit (100):

Q: How does the QIAfilter Plasmid Midi Kit purify plasmid DNA from bacterial cultures?

A: The kit employs alkaline lysis to break open E. coli cells, followed by anion-exchange chromatography using QIAfilter cartridges and midi spin columns for rapid and efficient DNA purification.

Q: What sample types and plasmid yields can I expect using this kit?

A: This kit is optimized for bacterial cell cultures of Escherichia coli and typically yields up to 100 g of high-copy plasmid DNA per preparation.

Q: When should I use the QIAfilter Plasmid Midi Kit over other purification kits?

A: Choose this kit for midi-scale plasmid preparations when you need rapid processing (6090 minutes per prep) and high-purity DNA suited for sensitive applications such as transfection, cloning, or sequencing.

Q: Where should the kit be stored, and what are the storage precautions?

A: Store the kit at room temperature between 1525C. Avoid exposing it to moisture or direct sunlight to maintain reagent quality and kit performance.

Q: What is the process for using the QIAfilter Plasmid Midi Kit?

A: The process involves resuspending bacterial cells, performing alkaline lysis, neutralizing and clarifying lysates with the QIAfilter cartridge, binding DNA to the anion-exchange column, washing, and eluting DNA in 15 ml final volume.

Q: How does the included QIAfilter cartridge benefit my workflow?

A: The integrated cartridge speeds up lysate clarification by rapid filtration, reducing manual steps and improving overall processing times while maintaining high yield and purity.

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