Qiagen REPLI-g Advanced DNA Single Cell Kit (96)

Qiagen REPLI-g Advanced DNA Single Cell Kit (96)

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Product Details:

Shelf Life See kit label (usually 1224 months upon receipt)
Features High-coverage, high-fidelity DNA amplification from single or few cells; robust MDA-based technology optimized for advanced genomics workflows; contamination control
Usage Type Laboratory
Function Isolate and amplify DNA from single cells
Storage Instructions Store at 15C to 25C (freezer)
Instruments Type DNA Preparation/Amplification Kit
Accuracy High-fidelity whole genome amplification
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Qiagen REPLI-g Advanced DNA Single Cell Kit (96) Price And Quantity

Qiagen REPLI-g Advanced DNA Single Cell Kit (96) Product Specifications

Wall Mounted No
Shelf Life See kit label (usually 1224 months upon receipt)
Features High-coverage, high-fidelity DNA amplification from single or few cells; robust MDA-based technology optimized for advanced genomics workflows; contamination control
Technology Whole Genome Amplification (WGA) by Multiple Displacement Amplification (MDA)
Color White (box and vials as supplied)
Real-Time Operation No
Material Kit components (reagents, buffers, enzymes)
Accuracy High-fidelity whole genome amplification
Equipment Type DNA Amplification Kit
Instruments Type DNA Preparation/Amplification Kit
Portable Yes (kit-based)
Condition New
Use Single cell DNA isolation and whole genome amplification
Operating Type Manual
Usage Type Laboratory
Function Isolate and amplify DNA from single cells
Storage Instructions Store at 15C to 25C (freezer)

Product Description

Brand Qiagen

Product Type PCR Kit

Pack Size 96 Wells

CAT No 150365

Application Molecular Reagent

DNA sequence analysis and genotyping of biological samples using next-generation sequencing (NGS) platforms is often limited by the amount of available sample. The REPLI-g Advanced DNA Single Cell Kit has been specifically optimized for use in sensitive microarray and NGS applications, providing high uniformity, with minimal allelic dropout, when starting with single cells or purified genomic DNA. Buffers and reagents undergo a unique, controlled decontamination procedure to minimize contaminating DNA and ensure highly reliable results. Accurate amplification of genomes with negligible sequence bias and minimal genomic dropouts is achieved with innovative Multiple Displacement Amplification (MDA) technology

Robust Whole Genome Amplification From Single Cells

The Qiagen REPLI-g Advanced DNA Single Cell Kit employs advanced multiple displacement amplification technology to generate highly representative, high-coverage DNA from individual cells. This enables researchers to conduct reliable genomic analyses from minimal starting material, making it ideal for studying rare or limited samples across human, animal, or other cell types.

Optimized for Genomics and Advanced Workflows

Engineered for compatibility with next-generation sequencing (NGS), genotyping, and other genomic assays, this kit maximizes the utility of precious samples. Its spin-column-free, manual workflow significantly reduces contamination risks, ensuring accurate results for critical applications such as single-cell genomics and rare sample analysis.

FAQ's of Qiagen REPLI-g Advanced DNA Single Cell Kit (96):

Q: How does the Qiagen REPLI-g Advanced DNA Single Cell Kit achieve whole genome amplification from a single cell?

A: This kit uses an isothermal, multiple displacement amplification (MDA) process powered by Phi29 DNA polymerase. The method can amplify DNA from inputs as low as a single cell, yielding up to 40 g of high-fidelity DNA for downstream applications.

Q: What are the primary applications of the REPLI-g Advanced DNA Single Cell Kit?

A: The kit is designed for genomics research, including single-cell sequencing, rare sample analysis, and genetic studies that require high-quality, whole genome DNA amplification from minimal input material.

Q: When should I choose this kit over other DNA amplification kits?

A: Select this kit when working with single cells or ultra-low DNA quantities, especially when high-coverage, accurate genome representation and minimal contamination are crucial for downstream analyses like NGS or genotyping.

Q: Where can this kit be used effectively?

A: The kit is intended for laboratory use in settings such as research institutes, genomics facilities, and clinical research labs requiring precise single-cell DNA amplification.

Q: What is the process for using the REPLI-g Advanced DNA Single Cell Kit?

A: After lysing the single cell and neutralizing the reaction, the DNA is amplified isothermally using the kit's pre-formulated buffers and enzymes, with the entire process taking approximately 8 hours without the need for a spin column.

Q: How should the kit be stored, and what is its shelf life?

A: Store the kit at -15C to -25C upon receipt. Refer to the kit label for shelf life, which typically ranges from 12 to 24 months.

Q: What are the benefits of using this kit for single-cell genomics?

A: Researchers benefit from simplified workflows, robust contamination control, reliable high-yield DNA amplification, and compatibility with a wide range of downstream genomic analyses, all while working from extremely limited sample inputs.

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